外源褪黑素对UV-B辐射下马铃薯光合、荧光特性的影响

doi:10.7668/hbnxb.20192053

摘要/Abstract

摘要： 增强UV-B辐射会对植物造成损伤，褪黑素可以增强植物抗逆性。通过不同浓度褪黑素处理不同UV-B辐射下的马铃薯幼苗，测定其株高、光合参数（Pn、Gs、Ci、Tr）、荧光参数（Fv/Fm、ETR、qP、NPQ）、1，5-二磷酸核酮糖羧化酶（Rubisco）和磷酸烯醇式丙酮酸羧化酶（PEPC），分析褪黑素对UV-B辐射下马铃薯光合、荧光特性的影响，为揭示褪黑素对UV-B辐射下马铃薯植株的防御机制提供科学依据。以马铃薯品种合作88为试验材料，采用自然光照（CK）和3个增强的UV-B辐射（2.5，5.0，7.5 KJ/（m²·d）），在4种光照条件下分别设置0，25，50，100，150，200 μmol/L的褪黑素浓度处理马铃薯幼苗。马铃薯受到增强UV-B辐射后株高、1，5-二磷酸核酮糖羧化酶、磷酸烯醇式丙酮酸羧化酶、光合参数、荧光参数（除NPQ）都降低，施加褪黑素后有所升高。褪黑素能提高马铃薯对UV-B辐射的抗逆性，使其植株增高，光合能力增强，但过高的褪黑素浓度也会对植物造成一定的损伤。

关键词: 马铃薯, 褪黑素, UV-B辐射, 光合和荧光特性, Rubisco, PEPC

Abstract: Enhanced UV-B radiation can cause damage to plants, and melatonin can enhance plant resistance. At present, there is no research report on the effect of melatonin on potatoes under UV-B radiation, so this experiment measured plant height, photosynthetic parameters(Pn, Gs, Ci, and Tr), fluorescence parameters(Fv/Fm, ETR, qP, and NPQ), 1, 5-bisphosphate ribulose carboxylase(Rubisco) and phosphoenolpyruvate carboxylase(PEPC), to analyze the effect of melatonin on the photosynthetic and fluorescence characteristics of potatoes under UV-B radiation, provide scientific basis for revealing the defense mechanism of melatonin against potato plants under UV-B radiation. The potato variety Hezuo 88 planted in greenhouse was used as experimental material. The concentrations of melatonin were set at 0, 25, 50, 100, 150, 200 μmol/L respectively under natural light(CK) and three enhanced UV-B radiation((2.5, 5.0, 7.5 KJ/(m²·d)).The plant height, Rubisco, PEPC, photosynthetic parameters and fluorescence parameters (except NPQ) of potato decreased after exposure to enhanced UV-B radiation, but increased after exposure to melatonin.Melatonin can improve the resistance of potato to UV-B radiation, increase the plant height and enhance its photosynthetic capacity. However, the trend of these indicators rising first and then decreasing also indicates that excessive melatonin concentration will also cause certain damage to plants.

Key words: Potato, Melatonin, UV-B radiation, Photosynthetic and fluorescent properties, Rubisco PEPC

中图分类号:

S532.01

万丽嫱, 李光达, 和秋兰, 王正维, 张航, 海梅荣. 外源褪黑素对UV-B辐射下马铃薯光合、荧光特性的影响[J]. 华北农学报, 2021, 36(4): 116-123. doi: 10.7668/hbnxb.20192053.

WAN Liqiang, LI Guangda, HE Qiulan, WANG Zhengwei, ZHANG Hang, HAI Meirong. Effects of Exogenous Melatonin on Photosynthesis and Fluorescence Characteristics of Potato under UV-B Radiation[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(4): 116-123. doi: 10.7668/hbnxb.20192053.

http://www.hbnxb.net/CN/Y2021/V36/I4/116

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