6个马铃薯新品系的细胞学及SSR分析

doi:10.7668/hbnxb.20191721

摘要/Abstract

摘要： 为明确6个马铃薯新品系NNS-P 11、NNS-H 6、NNS-H 4、NNS-C 10、NNS-L 9和NNS-Y 40的分子细胞遗传学差异，对下一步新品种育成鉴定和登记利用提供依据。以马铃薯材料MIN-021作对照，利用常规压片法和SSR分子标记技术，对其花粉育性、PMCMⅠ染色体配对构型及SSR进行了比较分析。结果表明：6个马铃薯新品系间花粉育性有一定差异，新品系NNS-Y 40的花粉育性较低（39.95%），新品系NNS-P 11、NNS-H 4、NNS-C 10的花粉育性较高（81.12%，77.94%，77.76%），新品系NNS-H 6和NNS-L 9花粉育性居中（67.27%，69.71%）。6个新品系染色体配对构型依次为1.08 Ⅰ+14.89 Ⅱ+1.18 Ⅲ+3.40 Ⅳ、2.35 Ⅰ+11.96 Ⅱ+3.59 Ⅲ+2.74 Ⅳ、2.10 Ⅰ+14.21 Ⅱ+1.35 Ⅲ +3.02 Ⅳ、3.31 Ⅰ+13.98 Ⅱ+ 2.99 Ⅲ+1.94 Ⅳ、3.45 Ⅰ+12.94 Ⅱ+2.81 Ⅲ+2.56 Ⅳ和8.23 Ⅰ+6.92 Ⅱ+6.07 Ⅲ+1.93 Ⅳ。试验筛选出SSR适宜引物10对，对各新品系及对照材料基因组DNA扩增，获得82个SSR多态性条带，多态性比率占78.10%。各材料的遗传距离（GD）变幅为0.244 4～0.687 5，以GD值0.40为基准，划分为3类：第一类为新品系NNS-P 11、NNS-L 9、NNS-Y 40和对照MIN-021，第二类为新品系NNS-H 6和NNS-H 4，新品系NNS-C 10单独为一类。试验建立了能明确鉴别7个供试材料的SSR指纹图。

关键词: 马铃薯, 花粉育性, 染色体配对构型, SSR分析

Abstract: In order to determine the difference of molecular and cellular genetics of six new potato strains (NNS-P 11, NNS-H 6,NNS-H 4, NNS-C 10, NNS-L 9 and NNS-Y 40), with MIN-021 as control material, conventional compression and SSR molecular labeling were used to compare and analyze the traits of the six new strains, include pollen fertility, PMCMⅠ chromosome pairing behavior, SSR fingerprint characteristics. This would provide the basis for further breeding, identification and registration of new potato varieties. The results showed that:There were some differences in pollen fertility among six new potato strains. The new strain NNS-Y 40 had the lowest pollen fertility (39.95%), the new strains NNS-P 11, NNS-H 4, NNS-C 10 had the highest pollen fertility (81.12%, 77.94%, 77.76%), the new strains NNS-H 6 and NNS-L 9 pollen fertility in the middle of the lowest and highest (67.27%, 69.71%). The chromosome pairing configuration of six new strains NNS-P 11, NNS-H 6, NNS-H 4, NNS-C 10, NNS-L 9 and NNS-Y 40 were 1.08Ⅰ+14.89Ⅱ+1.18Ⅲ+3.4 0Ⅳ, 2.35Ⅰ+11.96Ⅱ+3.59Ⅲ+2.74Ⅳ, 2.10Ⅰ+14.21Ⅱ+1.35Ⅲ+3.02Ⅳ, 3.31Ⅰ+13.98Ⅱ+2.99Ⅲ+1.94Ⅳ, 3.45Ⅰ+12.94Ⅱ+2.81Ⅲ+2.56Ⅳ and 8.23Ⅰ+6.92Ⅱ+6.07Ⅲ+1.93Ⅳ. The genomic DNA of six potato strains and control sample was amplified with 10 pairs SSR selected primers and 82 polymorphic bands were obtained, with the polymorphic ratio was 78.10%. The genetic distance (GD) of new potato strains ranged from 0.244 4-0.687 5. Base on 0.40 genetic distance, seven test materials were divided into three categories:the first category was new strains NNS-P 11, NNS-L 9, NNS-Y 40 and MIN-021, the second category was new strain NNS-H 6 and NNS-H 4, and the third category was new strain NNS-C 10. In addition, a SSR fingerprint was established to distinguish 7 test materials.

Key words: Potato, Pollen fertility, Chromosome configuration, SSR analysis

中图分类号:

S532.03

李靓, 于卓, 于肖夏, 杨东升, 卢倩倩, 李景伟, 李佳奇, 刘宇飞. 6个马铃薯新品系的细胞学及SSR分析[J]. 华北农学报, 2021, 36(2): 74-80. doi: 10.7668/hbnxb.20191721.

LI Liang, YU Zhuo, YU Xiaoxia, YANG Dongsheng, LU Qianqian, LI Jingwei, LI Jiaqi, LIU Yufei. Analysis of Cytology and SSR for 6 New Potato Strains[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(2): 74-80. doi: 10.7668/hbnxb.20191721.

http://www.hbnxb.net/CN/Y2021/V36/I2/74

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