摘要： 为了选育抗多种病害的水稻新品种,保证水稻的稳产高产,利用分子标记辅助选择技术,对378份通过常规育种技术与花药培养相结合培育的聚合 Xa23、 Stvb-i、 Pi-1 抗病基因的花培后代进行试验研究。结果表明:分子标记辅助选择可快速实现 Xa23、 Pi-1 与 Stvb-i 抗3种病害基因的聚合,经PCR检测与抗病鉴定,培育出10份抗性遗传稳定、表达高效的粳稻新种质资源。在利用分子标记辅助选择基因型的同时,仍然需要与传统的人工抗病鉴定的表现型结果相结合,才能保证选择的准确率高。

关键词: 水稻, 分子标记辅助选择, 花药培养, 基因聚合, 抗病

Abstract: In order to breed new disease-resistant varieties and ensure stable and high-yields of rice, the study on 378 anther culture descendants produced by conventional and anther culture technology was carried out using molecular marker-assisted method.The results showed that three resistance genes of Xa23, Pi-1 and Stvb-i could be polymerized quickly by molecular marker-assisted method.Though the PCR detection and identification of disease resistance, 10 new seeds with stabilized hereditary and high level expression were obtained.The use of molecular marker assisted selection of genotypes at the same time, still needed to phenotype and artificial identification of resistance of the traditional combination, so as to ensure the high accuracy of selected.

Key words: Rice, Marker-assisted selection, Anther culture, Gene pyramiding, Resistance

中图分类号: 

• S511