华北农学报 ›› 2015, Vol. 30 ›› Issue (1): 97-102. doi: 10.7668/hbnxb.2015.01.016

所属专题: 生物技术

• 论文 • 上一篇    下一篇

津田芜菁泛素结合酶BrUBC11基因的克隆及表达分析

蒋忠荣, 申飞, 刘志, 郭瑞, 李桥, 闫海芳   

  1. 东北林业大学 生命科学学院, 黑龙江 哈尔滨 150040
  • 收稿日期:2014-11-27 出版日期:2015-02-28
  • 通讯作者: 闫海芳(1976-),女,山西临汾人,副教授,博士,主要从事植物发育生物学研究。
  • 作者简介:蒋忠荣(1994-),男,四川南充人,主要从事植物发育生物学研究。
  • 基金资助:
    中央高校基本科研业务费专项(DL12CA10);东北林业大学大学生创新创业训练项目(201310225115);国家基础科学人才培养基金—科研训练及科研能力提高项目(J1210053)

Cloning and Expression Analysis of Ubiquitin-conjugating Enzyme 11(BrUBC11)in Brassica rapa L.subsp. Rapa Tsuda

JIANG Zhong-rong, SHEN Fei, LIU Zhi, GUO Rui, LI Qiao, YAN Hai-fang   

  1. College of Life Sciences, Northeast Forestry University, Harbin 150040, China
  • Received:2014-11-27 Published:2015-02-28

摘要: 为阐释津田芜菁 BrUBC11 基因的表达特性,克隆了津田芜菁 UBC11 基因的全长cDNA序列,命名为 BrUBC11, GenBank登录号为KM396887。其cDNA全长685 bp,ORF区全长447 bp,编码148个氨基酸的开放阅读框;亚细胞定位结果显示,BrUBC11-GFP定位于细胞核内,表明BrUBC11蛋白可能在细胞核中发挥其功能。荧光定量PCR检测 BrUBC11 在芜菁不同组织中的表达结果表明,该基因在花瓣中表达量最高,花蕾中次之,具有组织特异性。而且 BrUBC11 在芜菁白色根皮中的表达受长波紫外线(UV-A)诱导。研究结果显示,在芜菁中, UBC11 属于多功能基因,具有潜在的参与花发育和UV-A信号转导相关途径的功能。

关键词: 津田芜菁, UBC11, 基因克隆, 表达分析

Abstract: In order to elucidate the expression feature of UBC11 in Tsuda, cDNA of UBC11 gene was isolated from this plant.This gene was named BrUBC11 (GenBank accession No.KM396887). BrUBC1I was 685 bp in full length cDNA and 447 bp in full length open reading frame (ORF) encoding 148 amino acids.BrUBC11-GFP was localized to nucleus, indicating that BrUBC11 may play an important role in the nucleus.Quantitative-PCR analysis showed that the BrUBC11 was the highest expressed in the petal and less in bud.The expression of BrUBC11 in Tsuda exhibited tissue specificity.The expression of the BrUBC11 was induced by UV-A light in the swollen hypocotyls.These results indicated of that BrUBC11 had the potential to play a role in response to floral development and UV-A signal transduction pathways, was a multifunction gene.

Key words: Brassica rapa, UBC11, Gene cloning, Expressing analysis

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引用本文

蒋忠荣, 申飞, 刘志, 郭瑞, 李桥, 闫海芳. 津田芜菁泛素结合酶BrUBC11基因的克隆及表达分析[J]. 华北农学报, 2015, 30(1): 97-102. doi: 10.7668/hbnxb.2015.01.016.

JIANG Zhong-rong, SHEN Fei, LIU Zhi, GUO Rui, LI Qiao, YAN Hai-fang. Cloning and Expression Analysis of Ubiquitin-conjugating Enzyme 11(BrUBC11)in Brassica rapa L.subsp. Rapa Tsuda[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(1): 97-102. doi: 10.7668/hbnxb.2015.01.016.

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