亚麻主要产量相关性状的SSR关联分析

doi:10.7668/hbnxb.20192676

摘要/Abstract

摘要：

为了深入了解亚麻种质产量相关性状的遗传变异,以229份亚麻种质为材料,利用广义线性模型(GLM)和混合线性模型(MLM)的关联分析,以挖掘亚麻产量相关性状显著关联的SSR标记。结果表明,呼和浩特地区种植亚麻种质株高((58.40±5.67)cm)、单株果数((17.50±3.50)个)和单株粒质量((0.55±0.15)g)的平均值最大;在集宁地区分枝数((5.00±1.25)个)、果粒数((6.52±2.67)粒)和千粒质量((5.58±1.48)g)平均值最大。7个产量相关性状的表型变异系数为11.18%~18.29%。广义遗传力从大到小依次为千粒质量>果粒数>单株粒质量>株高>单株果数>分枝数>工艺长度。群体结构K=4时,229份亚麻种质分为4个群体。30对SSR引物共扩增出365条带,与亚麻7个产量相关性状进行GLM关联分析共检测到36个SSR标记,表型变异的解释率为1.15%(Lu146)~7.75%(Lu747);MLM关联分析共检测到23个SSR标记,表型变异的解释率为2.26%(Lu203a)~7.16%(Lu291)。在GLM和MLM下,单株粒质量、单株果数和分枝数同时检测到了Lu203a和Lua125a标记,分别分布在第12,2号染色体上。亚麻SSR标记能够很好地关联到产量相关性状,具有一定的解释率。

关键词: 亚麻, 产量, 关联分析, SSR

Abstract:

In order to deeply understand the genetic variation of yield related traits of flax germplasm,229 flax germplasm resources were used as materials,SSR markers that were significantly associated with yield related traits in flax were explored by association analysis of generalized linear model(GLM)and mixed linear model(MLM). The results showed that the indexes of plant height((58.40±5.67)cm),fruit number per plant(17.50±3.50)and grain weight per plant((0.55±0.15)g)of flax germplasm planted in Hohhot were the largest under four environments. In Jining area,the indexes of branch number(5.00±1.25),fruit grain number(6.52±2.67)and 1000-seed weight((5.58±1.48)g)were the largest. The phenotypic variation coefficients of the seven yield related traits were 11.18%—18.29%. The order of generalized heritability was 1000-seed weight > seeds per capsule>seeds weight per plant>plant height>capsule number per plant>branch number>stem length. When the population structure K=4,229 flax germplasm were divided into 4 groups. A total of 365 bands were amplified by 30 pairs of SSR primers,and 36 SSR markers were detected by GLM correlation analysis with 7 yield related traits of flax. The interpretation rate of phenotypic variation was 1.15%(Lu146)—7.75%(Lub747);MLM association analysis detected 23 SSR markers,and the interpretation rate of phenotypic variation was 2.26%(Lu203a)—7.16%(Lu291). Under GLM and MLM,Lu203a and Lua125a markers were detected on chromosome 12 and 2,respectively. It was concluded that SSR markers in flax could be well correlated with yield-related traits and had a certain explanatory rate.

Key words: Flax, Yield, Correlation analysis, SSR

伊六喜, 邬阳, 曹彦, 贾海滨, 斯钦巴特尔, 高凤云, 周宇, 贾霄云. 亚麻主要产量相关性状的SSR关联分析[J]. 华北农学报, 2022, 37(5): 52-61. doi: 10.7668/hbnxb.20192676.

YI Liuxi, WU Yang, CAO Yan, JIA Haibin, SIQIN Bateer, GAO Fengyun, ZHOU Yu, JIA Xiaoyun. SSR Correlation Analysis of Main Yield Related Traits in Flax[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 52-61. doi: 10.7668/hbnxb.20192676.

http://www.hbnxb.net/CN/Y2022/V37/I5/52

图/表 12

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种植区 Location	海拔/m Altitude	东经 East longitude	北纬 Northern latitude	年降雨量/mm Rainfall	日照时数/h Sunshine	播种时期/ (月-日) Sowing	收获时期/ (月-日) Harvest
呼和浩特 Hohhot	1 056	111°48'	40°49'	410	3 000	04-28	08-20
太仆寺旗 Taibus Banner	1 600	115°10'	41°35'	350	2 937	05-25	09-17
集宁 Jining	1 417	113°10'	40°01″	384	3 130	05-15	09-08
伊犁 Ili	670	81°12'	43°47'	417	2 890	03-20	07-12

种植区 Location	海拔/m Altitude	东经 East longitude	北纬 Northern latitude	年降雨量/mm Rainfall	日照时数/h Sunshine	播种时期/ (月-日) Sowing	收获时期/ (月-日) Harvest
呼和浩特 Hohhot	1 056	111°48'	40°49'	410	3 000	04-28	08-20
太仆寺旗 Taibus Banner	1 600	115°10'	41°35'	350	2 937	05-25	09-17
集宁 Jining	1 417	113°10'	40°01″	384	3 130	05-15	09-08
伊犁 Ili	670	81°12'	43°47'	417	2 890	03-20	07-12

引物名称 Primer name	序列(5'—3') Sequence (5'—3')	GC含量/% GC content	引物名称 Primer name	序列(5'—3') Sequence (5'—3')	GC含量/% GC content
Lub4	TGGAAGTCAACGAGATCGAA	50.00	Lu400	GAATGGCTCCTCGAAAGATG	46.40
	ACAGCAGCCTCCGTGTTTAT			ATTAGACGGGGAGCTTGAGG
Lub13	CGAGGATGACAATGATGACG	46.63	Lu422	GTTAATCGCCCCTGAACTGA	55.87
	CAGCAGCAGCATCAGGTAAA			TTGCAGTTACAACAGCAGCA
Lua37	CACAGCACAGACACAGACCA	36.80	Lu462	AATGAGCACAACAACAGCAAG	39.53
	GGCGGCTTTAAGAAGTGAAA			AGCAGCTCTGGACTTGAGGA
Lua69	CTAAACCACACCCCCATCAC	50.97	Lu465	CAAGACTTGTAGGGCGGAAC	56.25
	AAAGTGGGGAAATTGGGCTA			CGTCGGCCTATGAGAAGAAC
Lua83B	CCCTCATTTTTCTCCTTCCA	50.26	Lu511	CATTGACCTCCCATTTCACC	44.76
	CAGGCGTTACAGTTTCCCATA			TCAAGGAAGGCTCGTTGTTC
Lua125a	GCCTTTGGAGGGCTTAACTT	43.72	Lu554	GGCCAAGGATATAGCACGAA	45.27
	ACAATCCCAACATTCCCAAA			TTGGACCTTAAGCCCAGATG
Lu146	AACCTGAACCAGACGAGCAT	42.32	Lu598	TAGAGGCCAGCTAGCAGCA	45.54
	AGGTGGATCCAGCAAGCTAA			AAAAGCTTCCCTTTGGTGGT
Lu176	TCCATCCTCTGCATTTGTGA	42.53	Lu661	AAGACAACAACCTGGGGAAA	52.36
	AAGACGAGTGCCCATTCCTA			GATTCAGCAGCCGAGAGTG
Lu203a	CCTTTTCACGCAGAGCTACC	48.98	Lu747	CGGCTGAGGATTACTTGTCG	56.00
	GCTTCCGTAATCCTCTTCCA			TAAACTCCACTTCCCCCAAC
Lu263	GCCGAAAGTTGAAGCATAGG	39.93	Lu765B	CCTCATTCCGCTCAGCAA	47.34
	TGTTGCTTGTTGGCAAACTG			CGAAAATGGGGAAGATGATG
Lu266	ACGACACCGGATTTATCTGC	50.48	Lu771	ATACTCCTCCGACGCTGATG	58.96
	ACGTGTCCTCCACATGCTCT			AACCTCGAAACGAATGATGC
Lu273	CGATGATCACTGGACGGATA	44.78	Lu785	CGAGGCATCATATTTTCTCTTG	33.33
	CATAGCTTCAAAGGCAGCAC			ATCAGCAATCAATCGCATCA
Lu291	GGAAATTCCAAGTTCCCAGT	37.18	Lu787	AAGACCACCACAAGGGACAG	59.69
	AGTTTCGCTATTCCGTCTGC			TGAACCATAGCGATCATCACA
Lu316	TCCTCGGAAGAAGAAGACGA	50.00	Lu840	ATTCCTTTTTGAGGGCGAGT	48.82
	GAGAGGAATCATGGCGGATA			ACAGCTGGAACTGGAGAGGA
Lu330	TCTTGTACATTGCGGCACTC	42.53	Lu849	CGACACAGCATTCAATGACC	50.00
	GCACCAGATGAGGAAGAGGA			CAGACCTTGGAGCTTTGGAG

引物名称 Primer name	序列(5'—3') Sequence (5'—3')	GC含量/% GC content	引物名称 Primer name	序列(5'—3') Sequence (5'—3')	GC含量/% GC content
Lub4	TGGAAGTCAACGAGATCGAA	50.00	Lu400	GAATGGCTCCTCGAAAGATG	46.40
	ACAGCAGCCTCCGTGTTTAT			ATTAGACGGGGAGCTTGAGG
Lub13	CGAGGATGACAATGATGACG	46.63	Lu422	GTTAATCGCCCCTGAACTGA	55.87
	CAGCAGCAGCATCAGGTAAA			TTGCAGTTACAACAGCAGCA
Lua37	CACAGCACAGACACAGACCA	36.80	Lu462	AATGAGCACAACAACAGCAAG	39.53
	GGCGGCTTTAAGAAGTGAAA			AGCAGCTCTGGACTTGAGGA
Lua69	CTAAACCACACCCCCATCAC	50.97	Lu465	CAAGACTTGTAGGGCGGAAC	56.25
	AAAGTGGGGAAATTGGGCTA			CGTCGGCCTATGAGAAGAAC
Lua83B	CCCTCATTTTTCTCCTTCCA	50.26	Lu511	CATTGACCTCCCATTTCACC	44.76
	CAGGCGTTACAGTTTCCCATA			TCAAGGAAGGCTCGTTGTTC
Lua125a	GCCTTTGGAGGGCTTAACTT	43.72	Lu554	GGCCAAGGATATAGCACGAA	45.27
	ACAATCCCAACATTCCCAAA			TTGGACCTTAAGCCCAGATG
Lu146	AACCTGAACCAGACGAGCAT	42.32	Lu598	TAGAGGCCAGCTAGCAGCA	45.54
	AGGTGGATCCAGCAAGCTAA			AAAAGCTTCCCTTTGGTGGT
Lu176	TCCATCCTCTGCATTTGTGA	42.53	Lu661	AAGACAACAACCTGGGGAAA	52.36
	AAGACGAGTGCCCATTCCTA			GATTCAGCAGCCGAGAGTG
Lu203a	CCTTTTCACGCAGAGCTACC	48.98	Lu747	CGGCTGAGGATTACTTGTCG	56.00
	GCTTCCGTAATCCTCTTCCA			TAAACTCCACTTCCCCCAAC
Lu263	GCCGAAAGTTGAAGCATAGG	39.93	Lu765B	CCTCATTCCGCTCAGCAA	47.34
	TGTTGCTTGTTGGCAAACTG			CGAAAATGGGGAAGATGATG
Lu266	ACGACACCGGATTTATCTGC	50.48	Lu771	ATACTCCTCCGACGCTGATG	58.96
	ACGTGTCCTCCACATGCTCT			AACCTCGAAACGAATGATGC
Lu273	CGATGATCACTGGACGGATA	44.78	Lu785	CGAGGCATCATATTTTCTCTTG	33.33
	CATAGCTTCAAAGGCAGCAC			ATCAGCAATCAATCGCATCA
Lu291	GGAAATTCCAAGTTCCCAGT	37.18	Lu787	AAGACCACCACAAGGGACAG	59.69
	AGTTTCGCTATTCCGTCTGC			TGAACCATAGCGATCATCACA
Lu316	TCCTCGGAAGAAGAAGACGA	50.00	Lu840	ATTCCTTTTTGAGGGCGAGT	48.82
	GAGAGGAATCATGGCGGATA			ACAGCTGGAACTGGAGAGGA
Lu330	TCTTGTACATTGCGGCACTC	42.53	Lu849	CGACACAGCATTCAATGACC	50.00
	GCACCAGATGAGGAAGAGGA			CAGACCTTGGAGCTTTGGAG

性状 Trait	环境 Environment	最小值 Minimum	最大值 Maximum	平均值 Mean value	标准差 s	变异系数/% CV	广义遗传力/% h²
株高/cm	HS	37.20	90.50	58.40a	5.67	11.56	59.24
Plant height(PH)	JN	36.80	83.70	54.80bc	6.12	12.43
	XM	37.60	87.50	56.60b	5.68	10.32
	XJ	38.10	91.50	57.40ab	7.41	13.54
工艺长度/cm	HS	12.80	37.50	21.80a	5.65	14.67	48.85
Stem length(SL)	JN	11.50	32.40	20.10b	6.43	15.67
	XM	10.40	31.50	19.50bc	7.12	14.68
	XJ	9.50	32.70	22.30a	6.90	13.60
分枝数	HS	3.50	6.50	4.50ab	0.85	9.76	51.62
Branch number(BN)	JN	2.50	7.50	5.00a	1.25	12.45
	XM	2.50	7.00	4.25b	1.05	11.85
	XJ	3.00	6.75	4.75ab	0.97	10.68
单株果数	HS	13.50	35.45	17.50a	3.50	23.63	54.58
Capsule number per plant	JN	13.85	33.56	15.03bc	4.45	17.12
(CPP)	XM	12.55	34.23	16.15b	5.95	15.65
	XJ	10.25	35.65	16.55ab	6.25	16.76
果粒数	HS	3.90	8.25	6.15a	1.59	15.65	61.65
Seeds per capsule	JN	4.20	7.67	6.52a	2.67	13.31
(SPC)	XM	4.45	8.47	5.97bc	1.45	11.64
	XJ	4.25	7.50	6.25ab	2.56	10.85
千粒质量/g	HS	3.85	7.24	5.45bc	1.34	12.45	63.25
1000-seed weight	JN	3.25	7.65	5.58a	1.48	12.15
(TSW)	XM	3.08	7.24	5.17bc	1.79	13.67
	XJ	2.88	7.85	5.50ab	2.14	14.50
单株粒质量/g	HS	0.25	1.43	0.55a	0.15	19.70	59.58
Seeds weight per plant	JN	0.18	1.28	0.54ab	0.24	15.45
(SWPP)	XM	0.20	1.17	0.49bc	0.22	13.64
	XJ	0.21	1.31	0.50ab	0.18	14.85

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