湖羊<i>FITM2</i>基因多态性及其与生长性状的关联分析

doi:10.7668/hbnxb.20195349

华北农学报 ›› 2025, Vol. 40 ›› Issue (3): 173-181. doi: 10.7668/hbnxb.20195349

所属专题： 畜牧；

• 畜牧·水产·兽医 • 上一篇下一篇

湖羊FITM2基因多态性及其与生长性状的关联分析

孔得文¹, 王维民², 田慧彬², 张德印², 赵利明², 杨晓斌¹, 马宗武¹, 李成海³, 张健¹, 蒲萌茹², 曹佩亮¹, 李林庭¹, 李泓舰¹, 贾国星¹, 高飞³, 武万恩³, 王立忠³, 冯炼君², 肖子越², 张琪², 闫成琪², 高磊², 张小雪¹^,

¹ 甘肃农业大学动物科学技术学院,甘肃兰州 730070
² 兰州大学草地农业科技学院,甘肃兰州 730000
³ 金川集团股份有限公司居佳生态农业有限公司,甘肃金昌 737103

收稿日期:2024-08-13 出版日期:2025-07-04
通讯作者:
张小雪(1984—),女,湖北武汉人,副教授,博士,硕士生导师,主要从事动物遗传育种研究。
作者简介:
孔得文(2002—),男,甘肃临夏人,在读硕士,主要从事动物遗传育种研究。
基金资助:
国家自然科学基金项目(32260818); 国家自然科学基金项目(32372850); 国家重点研发计划青年科学家项目(2022YFD1302000); 甘肃省科技重大专项(22ZD6NC069); 武威市科技计划项目(WW23A03ZDQ001); 金昌居家节粮低脂湖羊的选育研究项目(JKQGJJ23Z202301)

Analysis of FITM2 Gene Polymorphism and Its Association with Growth Traits in Hu Sheep

KONG Dewen¹, WANG Weimin², TIAN Huibin², ZHANG Deyin², ZHAO Liming², YANG Xiaobin¹, MA Zongwu¹, LI Chenghai³, ZHANG Jian¹, PU Mengru², CAO Peiliang¹, LI Linting¹, LI Hongjian¹, JIA Guoxing¹, GAO Fei³, WU Wanen³, WANG Lizhong³, FENG Lianjun², XIAO Ziyue², ZHANG Qi², YAN Chengqi², GAO Lei², ZHANG Xiaoxue¹^,

¹ College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China
² College of Pastoral Agricultural Science and Technology,Lanzhou University,Lanzhou 730000,China
³ Jinchuan Group Co.,Ltd.Jujia Ecological Agriculture Co.,Ltd.,Jinchang 737103,China

Received:2024-08-13 Published:2025-07-04

摘要/Abstract

摘要：

脂肪储存诱导跨膜蛋白2 (FITM2) 在调节脂质储存和骨骼肌能量代谢中发挥着重要作用。为了扫描绵羊FITM2基因多态性,探究其与湖羊生长性状的关系,选取1 128只健康无病、表型记录准确的湖羊为试验群体,以绵羊FITM2基因为研究对象,首先运用qPCR技术对FITM2基因在不同组织中的表达差异进行分析,其次通过PCR扩增、Sanger测序和AQP基因分型技术对绵羊FITM2基因多态性进行检测,并与生长性状进行关联分析。研究结果表明,FITM2基因在湖羊不同组织中均有表达,其中在尾脂中的表达水平最高,且显著高于其他组织。FITM2基因多态性检测结果显示,在FITM2基因第1内含子中存在g.72704027 C>T突变位点。关联分析结果表明,CC基因型个体的100,120日龄体质量、140,160日龄体高、80,120,140,160日龄体长、100日龄胸围均显著高于TT基因型个体。综上,绵羊FITM2基因g.72704027 C>T突变位点可作为与湖羊生长性状相关的候选分子标记,为湖羊的遗传育种工作提供理论基础。

关键词: 湖羊, SNP, FITM2, 关联分析, 组织表达谱

Abstract:

Fat storage inducing transmembrane protein 2 (FITM2) plays an important role in regulating lipid storage and skeletal muscle energy metabolism.To scan the polymorphisms of the FITM2 gene in sheep and investigate its relationship with growth traits in Hu sheep,the experiment selected 1 128 healthy and disease-free,genetically well-documented Hu sheep as the experimental group,and used the FITM2 gene in sheep as the research object.First,the expression differences of the FITM2 gene in different tissues were analyzed using qPCR technology.Then,the genetic polymorphisms of the FITM2 gene in sheep were detected using PCR amplification,Sanger sequencing,and AQP (allele-specific quantitative PCR-based genotyping assay).Finally,the association between the genetic polymorphisms and growth traits was analyzed.The research results showed that the FITM2 gene was expressed in different tissues of Hu sheep,with the highest expression level in the tail fat and significantly higher than that in other tissues.The gene polymorphism detection results showed that there was a C>T mutation at position 72704027 in the first intron of the FITM2 gene.The results of correlation analysis showed that the 100,120 d body weight,140,160 d body height,80,120,140,160 d body length,and 100 d chest circumference of CC genotype individuals were significantly higher than those of TT genotype individuals.In summary,the FITM2 gene g.72704027 C>T mutation site in sheep can be used as a candidate molecular marker related to growth traits in Hu sheep,providing a theoretical basis for the genetic breeding work of Hu sheep.

Key words: Hu sheep, SNP, FITM2, Association analysis, Tissue expression profile

中图分类号:

813.3

孔得文, 王维民, 田慧彬, 张德印, 赵利明, 杨晓斌, 马宗武, 李成海, 张健, 蒲萌茹, 曹佩亮, 李林庭, 李泓舰, 贾国星, 高飞, 武万恩, 王立忠, 冯炼君, 肖子越, 张琪, 闫成琪, 高磊, 张小雪. 湖羊FITM2基因多态性及其与生长性状的关联分析[J]. 华北农学报, 2025, 40(3): 173-181. doi: 10.7668/hbnxb.20195349.

KONG Dewen, WANG Weimin, TIAN Huibin, ZHANG Deyin, ZHAO Liming, YANG Xiaobin, MA Zongwu, LI Chenghai, ZHANG Jian, PU Mengru, CAO Peiliang, LI Linting, LI Hongjian, JIA Guoxing, GAO Fei, WU Wanen, WANG Lizhong, FENG Lianjun, XIAO Ziyue, ZHANG Qi, YAN Chengqi, GAO Lei, ZHANG Xiaoxue. Analysis of FITM2 Gene Polymorphism and Its Association with Growth Traits in Hu Sheep[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(3): 173-181. doi: 10.7668/hbnxb.20195349.

http://www.hbnxb.net/CN/Y2025/V40/I3/173

图/表 9

参考文献 40

[1]	惠富平, 邵文挥, 孙雁冰. 湖羊繁育史及其当代保种考述[J]. 畜牧与兽医, 2022, 54(12):141-145.
	Hui F P, Shao W H, Sun Y B. Breeding history of Hu sheep and its contemporary breed conservation[J]. Animal Husbandry & Veterinary Medicine, 2022, 54(12):141-145.
[2]	孙义铭, 王文杰, 魏慧情, 陈瑶, 张孝安, 赵梦宇, 张子军, 任春环, 王冠军, 黄桠锋. 饲养模式对绵羊生长性能、屠宰性能以及瘤胃发酵的影响研究进展[J]. 中国畜牧杂志, 2024, 60(9):84-90.doi:10.19556/j.0258-7033.20231012-09.
	Sun Y M, Wang W J, Wei H Q, Chen Y, Zhang X A, Zhao M Y, Zhang Z J, Ren C H, Wang G J, Huang Y F. Research progress on the effects of feeding modes on growth performance,slaughter performance,and rumen fermentation of sheep[J]. Chinese Journal of Animal Science, 2024, 60(9):84-90.
[3]	张立昌. 中国牛羊肉进口贸易及消费现状研究[D]. 乌鲁木齐: 新疆农业大学, 2023. doi:10.27431/d.cnki.gxnyu.2023.000844.
	Zhang L C. Research on the current situation of import trade and consumption of beef and mutton in China[D]. Urumqi: Xinjiang Agricultural University, 2023.
[4]	Sari C I, Eikelis N, Head G A, Schlaich M, Meikle P, Lambert G, Lambert E. Android fat deposition and its association with cardiovascular risk factors in overweight young males[J]. Frontiers in Physiology, 2019, 10:1162.doi:10.3389/fphys.2019.01162. pmid: 31620011
[5]	李军, 金海. 2023年我国肉羊产业发展概况、未来发展趋势及建议[J]. 中国畜牧杂志, 2024, 60(3):322-328.doi:10.19556/j.0258-7033.20240206-05.
	Li J, Jin H. General situation,future development trend and suggestions of mutton sheep industry in China in 2023[J]. Chinese Journal of Animal Science, 2024, 60(3):322-328.
[6]	Zhang X Y, Liu C Y, Kong Y Y, Li F D, Yue X P. Effects of intramuscular fat on meat quality and its regulation mechanism in Tan sheep[J]. Frontiers in Nutrition, 2022, 9:908355.doi:10.3389/fnut.2022.908355.
[7]	Schumacher M, DelCurto-Wyffels H, Thomson J, Boles J. Fat deposition and fat effects on meat quality-a review[J]. Animals, 2022, 12(12):1550.doi:10.3390/ani12121550.
[8]	Zeng X W, Wang W M, Zhang D Y, Li X L, Zhang Y K, Zhao Y, Zhao L M, Wang J H, Xu D, Cheng J B, Li W X, Zhou B B, Lin C C, Yang X B, Zhai R, Ma Z W, Liu J, Cui P P, Weng X X, Wu W W, Zhang X X, Zheng W X. Polymorphism and expression level of the FADS3 gene and associated with the growth traits in Hu sheep[J]. Animal Biotechnology, 2023, 34(9):4793-4802.doi:10.1080/10495398.2023.2196313.
[9]	Tuersuntuoheti M, Zhang J H, Zhou W, Zhang C L, Liu C J, Chang Q Q, Liu S D. Exploring the growth trait molecular markers in two sheep breeds based on genome-wide association analysis[J]. PLoS One, 2023, 18(3):e0283383.doi:10.1371/journal.pone.0283383.
[10]	Zhang D Y, Zhang X X, Li F D, La Y F, Li G Z, Zhang Y K, Li X L, Zhao Y, Song Q Z, Wang W M. The association of polymorphisms in the ovine PPARGC1B and ZEB2 genes with body weight in Hu sheep[J]. Animal Biotechnology, 2022, 33(1):90-97.doi:10.1080/10495398.2020.1775626.
[11]	Kadereit B, Kumar P, Wang W J, Miranda D, Snapp E L, Severina N, Torregroza I, Evans T, Silver D L. Evolutionarily conserved gene family important for fat storage[J]. Proceedings of the National Academy of Sciences of the United States of America, 2008, 105(1):94-99.doi:10.1073/pnas.0708579105. pmid: 18160536
[12]	秦朋云. FITM2基因对绵羊脂肪代谢调控功能和机制的研究[D]. 太谷: 山西农业大学, 2022.doi:10.27285/d.cnki.gsxnu.2022.000284.
	Qin P Y. Study on the regulatory function and mechanism of FITM2 gene on fat metabolism in sheep[D]. Taigu: Shanxi Agricultural University, 2022.
[13]	Wang G P, Chen A Q, Wu Y, Wang D L, Chang C F, Yu G Y. Fat storage-inducing transmembrane proteins:beyond mediating lipid droplet formation[J]. Cellular & Molecular Biology Letters, 2022, 27(1):98.doi:10.1186/s11658-022-00391-z.
[14]	Miranda D A, Koves T R, Gross D A, Chadt A, Al-Hasani H, Cline G W, Schwartz G J, Muoio D M, Silver D L. Re-patterning of skeletal muscle energy metabolism by fat storage-inducing transmembrane protein 2[J]. Journal of Biological Chemistry, 2011, 286(49):42188-42199.doi:10.1074/jbc.M111.297127. pmid: 22002063
[15]	Bond L M, Ibrahim A, Lai Z W, Walzem R L, Bronson R T, Ilkayeva O R, Walther T C, Farese R V. Fitm2 is required for ER homeostasis and normal function of murine liver[J]. Journal of Biological Chemistry, 2023, 299(3):103022.doi:10.1016/j.jbc.2023.103022.
[16]	Moreira G C M, Boschiero C, Cesar A S M, Reecy J M, Godoy T F, Trevisoli P A, Cantão M E, Ledur M C, Ibelli A M G, Peixoto J O, Moura A S A M T, Garrick D, Coutinho L L. A genome-wide association study reveals novel genomic regions and positional candidate genes for fat deposition in broiler chickens[J]. BMC Genomics, 2018, 19(1):374.doi:10.1186/s12864-018-4779-6. pmid: 29783939
[17]	Gupta R K, Attie A D. Introduction to the thematic review series:adipose biology[J]. Journal of Lipid Research, 2019, 60(10):1646-1647.doi:10.1194/jlr.IN119000337. pmid: 31413067
[18]	Cheng J B, Wang W M, Zhang D Y, Zhang Y K, Li X L, Zhao Y, Xu D, Zhao L M, Li W X, Wang J H, Zhou B B, Lin C C, Yang X B, Zhang X X. Identification of polymorphic loci in OSMR and GHR genes and analysis of their association with growth traits in sheep[J]. Animal Biotechnology, 2023, 34(7):2546-2553.doi:10.1080/10495398.2022.2105227.
[19]	孙国权, 高树新, 吴慧光, 李俊雅, 丽春, 王景山, 王玉泉, 王国富. 解偶联蛋白1、2和3基因在中国西门塔尔牛组织器官中的表达水平及其与胴体品质关系分析[J]. 华北农学报, 2014, 29(4):116-120.doi:10.7668/hbnxb.2014.04.019.
	Sun G Q, Gao S X, Wu H G, Li J Y, Li C, Wang J S, Wang Y Q, Wang G F. Analyze the ucp1,ucp2,ucp3 genes expression level in tissues/organs and the relationship with the carcass traits in Chinese Simmental cattle[J]. Acta Agriculturae Boreali-Sinica, 2014, 29(4):116-120.
[20]	Zhao H Y, Wu X F, Cai H F, Pan C Y, Lei C Z, Chen H, Lan X Y. Genetic variants and effects on milk traits of the caprine paired-like homeodomain transcription factor 2(PITX2)gene in dairy goats[J]. Gene, 2013, 532(2):203-210.doi:10.1016/j.gene.2013.09.062.
[21]	孙胜祥, 张建明, 江小帆. 不同生长阶段湖羊体重与体尺指标相关性研究[J]. 山东畜牧兽医, 2024, 45(4):4-6.
	Sun S X, Zhang J M, Jiang X F. Study on the correlation between body weight and body size of Hu sheep at different growth stages[J]. Shandong Journal of Animal Science and Veterinary Medicine, 2024, 45(4):4-6.
[22]	Abousoliman I, Reyer H, Oster M, Murani E, Mohamed I, Wimmers K. Genome-wide analysis for early growth-related traits of the locally adapted Egyptian barki sheep[J]. Genes, 2021, 12(8):1243.doi:10.3390/genes12081243.
[23]	Murawska-Ciałowicz E. Adipose tissue-morphological and biochemical characteristic of different depots[J]. Postepy Higieny i Medycyny Doswiadczalnej (Online), 2017, 71(1):466-484.doi:10.5604/01.3001.0010.3829.
[24]	Gross D A, Snapp E L, Silver D L. Structural insights into triglyceride storage mediated by fat storage-inducing transmembrane (FIT) protein 2[J]. PLoS One, 2010, 5(5):e10796.doi:10.1371/journal.pone.0010796.
[25]	Basse A L, Dixen K, Yadav R, Tygesen M P, Qvortrup K, Kristiansen K, Quistorff B, Gupta R, Wang J, Hansen J B. Global gene expression profiling of brown to white adipose tissue transformation in sheep reveals novel transcriptional components linked to adipose remodeling[J]. BMC Genomics, 2015, 16(1):215.doi:10.1186/s12864-015-1405-8.
[26]	Cannon B, Nedergaard J. Brown adipose tissue:function and physiological significance[J]. Physiological Reviews, 2004, 84(1):277-359.doi:10.1152/physrev.00015.2003.
[27]	张德荣. 脂联素及其受体介导绵羊肌内、内脏和皮下脂肪细胞生脂基因表达与分子调控机理研究[D]. 兰州: 甘肃农业大学, 2019.doi:10.27025/d.cnki.ggsnu.2019.000009.
	Zhang D R. Adiponectin and adiponectin receptor mediated adipogenic gene expression and molecular regulation mechanism in sheep muscle,visceral and subcutaneous adipocytes[D]. Lanzhou: Gansu Agricultural University, 2019.
[28]	Lu W H, Feng W J, Lai J Y, Yuan D L, Xiao W F, Li Y S. Role of adipokines in sarcopenia[J]. Chinese Medical Journal, 2023, 136(15):1794-1804.doi:10.1097/cm9.0000000000002255.
[29]	Fiaschi T, Cirelli D, Comito G, Gelmini S, Ramponi G, Serio M, Chiarugi P. Globular adiponectin induces differentiation and fusion of skeletal muscle cells[J]. Cell Research, 2009, 19(5):584-597.doi:10.1038/cr.2009.39. pmid: 19350052
[30]	Gamberi T, Modesti A, Magherini F, D'Souza D M, Hawke T, Fiaschi T. Activation of autophagy by globular adiponectin is required for muscle differentiation[J]. Biochimica et Biophysica Acta, 2016, 1863(4):694-702.doi:10.1016/j.bbamcr.2016.01.016. pmid: 26826036
[31]	Ge Y C, Zhou M, Chen C, Wu X J, Wang X B. Role of AMPK mediated pathways in autophagy and aging[J]. Biochimie, 2022, 195:100-113.doi:10.1016/j.biochi.2021.11.008.
[32]	Kang C, Ji L L. Role of PGC-1α signaling in skeletal muscle health and disease[J]. Annals of the New York Academy of Sciences, 2012, 1271(1):110-117.doi:10.1111/j.1749-6632.2012.06738.x.
[33]	Miranda D A, Kim J H, Nguyen L N, Cheng W, Tan B C, Goh V J, Tan J S Y, Yaligar J, Kn B P, Velan S S, Wang H Y, Silver D L. Fat storage-inducing transmembrane protein 2 is required for normal fat storage in adipose tissue[J]. Journal of Biological Chemistry, 2014, 289(14):9560-9572.doi:10.1074/jbc.M114.547687. pmid: 24519944
[34]	Saponaro C, Gaggini M, Carli F, Gastaldelli A. The subtle balance between lipolysis and lipogenesis:a critical point in metabolic homeostasis[J]. Nutrients, 2015, 7(11):9453-9474.doi:10.3390/nu7115475. pmid: 26580649
[35]	Nishihama N, Nagayama T, Makino S, Koishi R. Mice lacking fat storage-inducing transmembrane protein 2 show improved profiles upon pressure overload-induced heart failure[J]. Heliyon, 2019, 5(3):e01292.doi:10.1016/j.heliyon.2019.e01292.
[36]	陈晓敏, 李沂霖, 何炜诺, 卢焕聪, 廖嘉龙, 黄正, 柳广斌. 分子标记技术在山羊育种中的研究进展[J]. 中国草食动物科学, 2023, 43(6):45-51.doi:10.3969/j.issn.2095-3887.2023.06.009.
	Chen X M, Li Y L, He W N, Lu H C, Liao J L, Huang Z, Liu G B. Research progress of molecular marker technology in goat breeding[J]. China Herbivore Science, 2023, 43(6):45-51.
[37]	李旭静. 绵羊产肉性能相关基因的筛选与KASP基因分型方法的建立[D]. 保定: 河北农业大学, 2020. doi:10.27109/d.cnki.ghbnu.2020.000453.
	Li X J. Screening of genes related to sheep meat production performance and establishment of KASP gene typing method[D]. Baoding: Hebei Agricultural University, 2020.
[38]	Liu F, Calhoun B, Alam M S, Sun M M, Wang X C, Zhang C, Haldar K, Lu X. Case report:a synonymous VHL mutation (c.414A>G,p.Pro138Pro) causes pathogenic familial hemangioblastoma through dysregulated splicing[J]. BMC Medical Genetics, 2020, 21(1):42.doi:10.1186/s12881-020-0976-7.
[39]	Cheng J B, Zhang X X, Li F D, Yuan L F, Zhang D Y, Zhang Y K, Song Q Z, Li X L, Zhao Y, Xu D, Zhao L M, Li W X, Wang J H, Zhou B B, Lin C C, Yang X B, Wang W M. Detecting single nucleotide polymorphisms in MEF2B and UCP3 and elucidating their association with sheep growth traits[J]. DNA and Cell Biology, 2021, 40(12):1554-1562.doi:10.1089/dna.2021.0782.
[40]	Walsh I M, Bowman M A, Soto Santarriaga I F, Rodriguez A, Clark P L. Synonymous codon substitutions perturb cotranslational protein folding in vivo and impair cell fitness[J]. Proceedings of the National Academy of Sciences of the United States of America, 2020, 117(7):3528-3534.doi:10.1073/pnas.1907126117. pmid: 32015130

引物名称 Primer name	引物序列(5'—3') Primer sequence(5'—3')	产物大小/bp Product size	Tm/℃	用途 Usage
FITM2-F	CCAGCCCAGCAGACCGAAC	694	58.30	PCR
FITM2-R	ACGCTTTCACTTTCTTGAGGT
R-FITM2-F	TGGCACGGCTTCGACATCTCA	105	60.95	qPCR
R-FITM2-R	GTTCCGGTCCGTCTTCACCT
UXT-F	GCAAGTGGATTTGGGCTGTAAC	180	58.51	内参基因
UXT-R	ATGGAGTCCTTGGTGAGGCTGT
FITM2-Fam	GAAGGTGACCAAGTTCATGCTGTGAAGAGC			AQP
	AGGGATGTGGC
FITM2-Hex	GAAGGTCGGAGTCAACGGATTGGTGAAGAG
	CAGGGATGTGGT
FITM2-Rev	GGCATGACACGGGACTCTCC

引物名称 Primer name	引物序列(5'—3') Primer sequence(5'—3')	产物大小/bp Product size	Tm/℃	用途 Usage
FITM2-F	CCAGCCCAGCAGACCGAAC	694	58.30	PCR
FITM2-R	ACGCTTTCACTTTCTTGAGGT
R-FITM2-F	TGGCACGGCTTCGACATCTCA	105	60.95	qPCR
R-FITM2-R	GTTCCGGTCCGTCTTCACCT
UXT-F	GCAAGTGGATTTGGGCTGTAAC	180	58.51	内参基因
UXT-R	ATGGAGTCCTTGGTGAGGCTGT
FITM2-Fam	GAAGGTGACCAAGTTCATGCTGTGAAGAGC			AQP
	AGGGATGTGGC
FITM2-Hex	GAAGGTCGGAGTCAACGGATTGGTGAAGAG
	CAGGGATGTGGT
FITM2-Rev	GGCATGACACGGGACTCTCC

位点 Locus	基因型 Genotype	样本数 Sample No.	基因型频率 Genotype frequency	等位基因 Allele	等位基因频率 Allele frequency	He	Ho	Ne	PIC
FITM2	CC	640	0.57	C	0.74	0.38	0.62	1.61	0.31
g.72704027	CT	391	0.34
C>T	TT	97	0.09	T	0.26

位点 Locus	基因型 Genotype	样本数 Sample No.	基因型频率 Genotype frequency	等位基因 Allele	等位基因频率 Allele frequency	He	Ho	Ne	PIC
FITM2	CC	640	0.57	C	0.74	0.38	0.62	1.61	0.31
g.72704027	CT	391	0.34
C>T	TT	97	0.09	T	0.26

性状 Traits	日龄 Day	基因型 Genotype
性状 Traits	日龄 Day	CC	CT	TT
个体数 No.		635	389	97
体质量/kg	80	18.652±4.407a	18.422±4.297a	18.030±4.321a
Body weight	100	23.251±5.651a	23.017±5.523ab	22.347±5.434b
	120	28.515±6.656a	28.425±6.447ab	27.370±6.658b
	140	34.064±7.385a	34.084±7.037a	32.606±7.425b
	160	39.596±8.132a	39.438±7.664a	38.368±7.866a
	180	44.302±8.437a	44.167±7.978a	43.070±7.985a
体高/cm	80	53.532±2.994a	53.370±3.153a	53.521±3.223a
Body height	100	56.203±3.285a	56.147±3.319a	56.041±3.284a
	120	58.973±3.363a	58.869±3.184a	58.546±3.189a
	140	61.753±3.298a	61.618±3.303a	60.840±3.110b
	160	63.876±3.278a	63.855±3.162ab	63.191±3.289b
	180	65.372±3.280a	65.406±3.256a	65.103±3.112a
体长/cm	80	57.009±4.096a	56.405±4.095b	56.619±4.233ab
Body length	100	60.015±4.362a	59.680±4.129a	59.747±4.849a
	120	63.609±4.302a	63.433±4.167a	62.608±4.609b
	140	67.380±4.265a	67.221±4.312a	66.052±4.498b
	160	70.375±4.283a	70.201±4.250ab	69.376±4.077b
	180	72.429±4.298a	72.563±4.299a	71.809±4.320a
胸围/cm	80	59.509±4.853a	59.412±4.889a	59.072±5.473a
Chest circumference	100	63.739±4.904a	63.661±4.866ab	62.830±4.665b
	120	67.609±5.037ab	67.917±4.856a	66.820±5.278b
	140	72.577±5.334a	72.902±5.162a	72.113±5.488a
	160	77.357±5.635a	77.584±5.278a	76.856±5.893a
	180	81.689±5.965a	81.528±5.595a	80.943±5.700a
管围/cm	80	6.467±0.592a	6.460±0.581a	6.451±0.576a
Cannon circumference	100	6.858±0.593a	6.839±0.595a	6.759±0.505a
	120	7.334±0.657a	7.335±0.634a	7.253±0.716a
	140	7.634±0.704a	7.640±0.661a	7.530±0.718a
	160	7.950±0.685a	7.933±0.636a	7.869±0.674a
	180	8.193±0.629a	8.185±0.620a	8.113±0.640a

选择文件类型/文献管理软件名称

选择包含的内容

湖羊FITM2基因多态性及其与生长性状的关联分析

Analysis of FITM2 Gene Polymorphism and Its Association with Growth Traits in Hu Sheep

RichHTML

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 9

参考文献 40

相关文章 15

编辑推荐

Metrics

本文评价

[1]	马荆鄂, 陈玉洁, 雷文静, 许桥, 许继国, 许晶, 饶友生. 基于组学数据筛选康乐黄鸡8周龄体质量相关基因[J]. 华北农学报, 2025, 40(1): 224-231.
[2]	黄志强, 王维民, 张德印, 赵源, 张煜坤, 徐丹, 杨晓斌, 马宗武, 何丽娟, 蔡有鑫, 刘晓强, 张小雪. *湖羊ANO5基因多态性及其与脂肪沉积性状的关联分析*[J]. 华北农学报, 2025, 40(1): 207-214.
[3]	庞志远, 程宇坤, 郭小玲, 任毅, 耿洪伟. 水旱条件下小麦分蘖相关性状全基因组关联分析[J]. 华北农学报, 2024, 39(6): 64-75.
[4]	蔡有鑫, 王维民, 田慧彬, 张德印, 赵源, 张煜坤, 徐丹, 杨晓斌, 马宗武, 黄志强, 刘晓强, 何丽娟, 韩坤潮, 吴伟伟, 高飞, 王立忠, 张小雪. *湖羊CEL基因多态性及其与眼肌面积性状的关联分析*[J]. 华北农学报, 2024, 39(5): 231-238.
[5]	薛倩, 李国辉, 周成浩, 蒋一秀, 邢伟杰, 韩威. *汶上芦花鸡CDKN2A基因序列变异、生物信息学及组织表达分析*[J]. 华北农学报, 2024, 39(5): 219-230.
[6]	黄缓缓, 安洪周, 李魁英, 王延兵, 谷亦, 乔亚科, 高增玉. 玉米花丝花青苷显色性状的QTL定位[J]. 华北农学报, 2024, 39(4): 31-36.
[7]	赵杰, 牟丽明, 胡梦芸, 孙丽静, 李倩影, 王培楠, 李辉, 刘小民, 张颖君. 基于全基因组关联分析挖掘小麦耐草甘膦相关QTL[J]. 华北农学报, 2024, 39(3): 1-7.
[8]	张强龙, 吴森, 多杰才让, 唐燕花, 马杰. *欧拉羊角性状相关RXFP2基因SNPs检测及分析*[J]. 华北农学报, 2024, 39(2): 200-208.
[9]	李凯丽, 耿明状, 王胜, 郝维浩, 卢杰, 陈璨, 司红起. *安农1687抗条锈病候选基因TraesCS2A01G070700鉴定与分析*[J]. 华北农学报, 2024, 39(1): 150-155.
[10]	张曦, 刘祎, 钱玉源, 王广恩, 王寒菊, 焦秀芬, 解辉, 崔淑芳, 李俊兰. 陆地棉种质资源遗传多样性分析及初级核心种质构建[J]. 华北农学报, 2023, 38(S1): 26-33.
[11]	窦佳欣, 田甜, 王鹏, 刘媛, 陈涛, 张沛沛, 杨德龙. 小麦茎秆蔗糖积累转运全基因组关联分析[J]. 华北农学报, 2023, 38(5): 39-50.
[12]	李德生, 蒋秋斐, 封元, 王瑜, 冯小芳, 禹保军, 陈亚飞, 张迪, 王川川, 李蕾蕾, 顾亚玲, 张娟. *安格斯牛十字部高性状全基因组关联分析及PSEN1基因对成肌细胞增殖分化的影响*[J]. 华北农学报, 2023, 38(5): 206-217.
[13]	段宇轩, 崔京南, 徐善斌, 王敬国, 刘化龙, 杨洛淼, 贾琰, 辛威, 吴文申, 郑洪亮, 邹德堂. 粳稻粒型相关性状全基因组关联分析及候选基因挖掘[J]. 华北农学报, 2023, 38(5): 19-28.
[14]	韩浩园, 李涛, 李世凯, 宋小雨, 李君, 哈斯, 赵金艳, 魏红芳, 权凯. GJB6和PRKAA1基因多态性及其与槐山羊产羔数的关联分析[J]. 华北农学报, 2023, 38(4): 225-232.
[15]	刘慧, 许文静, 杨硕, 张威, 张红梅, 刘晓庆, 朱月林, 陈华涛. 菜用大豆有机酸的全基因组关联分析[J]. 华北农学报, 2023, 38(4): 74-82.

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

湖羊FITM2基因多态性及其与生长性状的关联分析

Analysis of FITM2 Gene Polymorphism and Its Association with Growth Traits in Hu Sheep

RichHTML

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 9

参考文献 40

相关文章 15

编辑推荐

Metrics

本文评价