华北农学报 ›› 2025, Vol. 40 ›› Issue (3): 173-181. doi: 10.7668/hbnxb.20195349

所属专题: 畜牧

• 畜牧·水产·兽医 • 上一篇    下一篇

湖羊FITM2基因多态性及其与生长性状的关联分析

孔得文1, 王维民2, 田慧彬2, 张德印2, 赵利明2, 杨晓斌1, 马宗武1, 李成海3, 张健1, 蒲萌茹2, 曹佩亮1, 李林庭1, 李泓舰1, 贾国星1, 高飞3, 武万恩3, 王立忠3, 冯炼君2, 肖子越2, 张琪2, 闫成琪2, 高磊2, 张小雪1,   

  1. 1 甘肃农业大学 动物科学技术学院,甘肃 兰州 730070
    2 兰州大学 草地农业科技学院,甘肃 兰州 730000
    3 金川集团股份有限公司居佳生态农业有限公司,甘肃 金昌 737103
  • 收稿日期:2024-08-13 出版日期:2025-07-04
  • 通讯作者:
    张小雪(1984—),女,湖北武汉人,副教授,博士,硕士生导师,主要从事动物遗传育种研究。
  • 作者简介:

    孔得文(2002—),男,甘肃临夏人,在读硕士,主要从事动物遗传育种研究。

  • 基金资助:
    国家自然科学基金项目(32260818); 国家自然科学基金项目(32372850); 国家重点研发计划青年科学家项目(2022YFD1302000); 甘肃省科技重大专项(22ZD6NC069); 武威市科技计划项目(WW23A03ZDQ001); 金昌居家节粮低脂湖羊的选育研究项目(JKQGJJ23Z202301)

Analysis of FITM2 Gene Polymorphism and Its Association with Growth Traits in Hu Sheep

KONG Dewen1, WANG Weimin2, TIAN Huibin2, ZHANG Deyin2, ZHAO Liming2, YANG Xiaobin1, MA Zongwu1, LI Chenghai3, ZHANG Jian1, PU Mengru2, CAO Peiliang1, LI Linting1, LI Hongjian1, JIA Guoxing1, GAO Fei3, WU Wanen3, WANG Lizhong3, FENG Lianjun2, XIAO Ziyue2, ZHANG Qi2, YAN Chengqi2, GAO Lei2, ZHANG Xiaoxue1,   

  1. 1 College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China
    2 College of Pastoral Agricultural Science and Technology,Lanzhou University,Lanzhou 730000,China
    3 Jinchuan Group Co.,Ltd.Jujia Ecological Agriculture Co.,Ltd.,Jinchang 737103,China
  • Received:2024-08-13 Published:2025-07-04

摘要:

脂肪储存诱导跨膜蛋白2 (FITM2) 在调节脂质储存和骨骼肌能量代谢中发挥着重要作用。为了扫描绵羊FITM2基因多态性,探究其与湖羊生长性状的关系,选取1 128只健康无病、表型记录准确的湖羊为试验群体,以绵羊FITM2基因为研究对象,首先运用qPCR技术对FITM2基因在不同组织中的表达差异进行分析,其次通过PCR扩增、Sanger测序和AQP基因分型技术对绵羊FITM2基因多态性进行检测,并与生长性状进行关联分析。研究结果表明,FITM2基因在湖羊不同组织中均有表达,其中在尾脂中的表达水平最高,且显著高于其他组织。FITM2基因多态性检测结果显示,在FITM2基因第1内含子中存在g.72704027 C>T突变位点。关联分析结果表明,CC基因型个体的100,120日龄体质量、140,160日龄体高、80,120,140,160日龄体长、100日龄胸围均显著高于TT基因型个体。综上,绵羊FITM2基因g.72704027 C>T突变位点可作为与湖羊生长性状相关的候选分子标记,为湖羊的遗传育种工作提供理论基础。

关键词: 湖羊, SNP, FITM2, 关联分析, 组织表达谱

Abstract:

Fat storage inducing transmembrane protein 2 (FITM2) plays an important role in regulating lipid storage and skeletal muscle energy metabolism.To scan the polymorphisms of the FITM2 gene in sheep and investigate its relationship with growth traits in Hu sheep,the experiment selected 1 128 healthy and disease-free,genetically well-documented Hu sheep as the experimental group,and used the FITM2 gene in sheep as the research object.First,the expression differences of the FITM2 gene in different tissues were analyzed using qPCR technology.Then,the genetic polymorphisms of the FITM2 gene in sheep were detected using PCR amplification,Sanger sequencing,and AQP (allele-specific quantitative PCR-based genotyping assay).Finally,the association between the genetic polymorphisms and growth traits was analyzed.The research results showed that the FITM2 gene was expressed in different tissues of Hu sheep,with the highest expression level in the tail fat and significantly higher than that in other tissues.The gene polymorphism detection results showed that there was a C>T mutation at position 72704027 in the first intron of the FITM2 gene.The results of correlation analysis showed that the 100,120 d body weight,140,160 d body height,80,120,140,160 d body length,and 100 d chest circumference of CC genotype individuals were significantly higher than those of TT genotype individuals.In summary,the FITM2 gene g.72704027 C>T mutation site in sheep can be used as a candidate molecular marker related to growth traits in Hu sheep,providing a theoretical basis for the genetic breeding work of Hu sheep.

Key words: Hu sheep, SNP, FITM2, Association analysis, Tissue expression profile

中图分类号: 

引用本文

孔得文, 王维民, 田慧彬, 张德印, 赵利明, 杨晓斌, 马宗武, 李成海, 张健, 蒲萌茹, 曹佩亮, 李林庭, 李泓舰, 贾国星, 高飞, 武万恩, 王立忠, 冯炼君, 肖子越, 张琪, 闫成琪, 高磊, 张小雪. 湖羊FITM2基因多态性及其与生长性状的关联分析[J]. 华北农学报, 2025, 40(3): 173-181. doi: 10.7668/hbnxb.20195349.

KONG Dewen, WANG Weimin, TIAN Huibin, ZHANG Deyin, ZHAO Liming, YANG Xiaobin, MA Zongwu, LI Chenghai, ZHANG Jian, PU Mengru, CAO Peiliang, LI Linting, LI Hongjian, JIA Guoxing, GAO Fei, WU Wanen, WANG Lizhong, FENG Lianjun, XIAO Ziyue, ZHANG Qi, YAN Chengqi, GAO Lei, ZHANG Xiaoxue. Analysis of FITM2 Gene Polymorphism and Its Association with Growth Traits in Hu Sheep[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(3): 173-181. doi: 10.7668/hbnxb.20195349.