华北农学报 ›› 2024, Vol. 39 ›› Issue (1): 150-155. doi: 10.7668/hbnxb.20194425

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• 资源环境·植物保护 • 上一篇    下一篇

安农1687抗条锈病候选基因TraesCS2A01G070700鉴定与分析

李凯丽1,2, 耿明状1, 王胜1, 郝维浩1, 卢杰1, 陈璨1, 司红起1   

  1. 1 安徽农业大学 农学院,安徽 合肥 230036
    2 安徽省农业科学院 水稻研究所,安徽 合肥 230036
  • 收稿日期:2023-08-27 出版日期:2024-03-03
  • 通讯作者:
    司红起(1972—),男,山东菏泽人,教授,博士,主要从事小麦品质改良与分子育种研究。
  • 作者简介:

    李凯丽(1994—),女,安徽安庆人,助理研究员,硕士,主要从事小麦品质改良与分子育种研究。

  • 基金资助:
    国家小麦产业技术体系专项(CARS-03); 安徽高校协同创新项目(GXXT-2019-033); 江苏现代作物协同创新中心项目(JCIC-MCP)

Identification and Analysis on the Resistance to Stripe Rust Candidate Gene of TraesCS2A01G070700 from Wheat Cultivar Annong 1687

LI Kaili1,2, GENG Mingzhuang1, WANG Sheng1, HAO Weihao1, LU Jie1, CHEN Can1, SI Hongqi1   

  1. 1 School of Agronomy,Anhui Agricultural University,Hefei 230036,China
    2 Rice Research Institute, Anhui Academy of Agricultural Sciences,Hefei 230036,China
  • Received:2023-08-27 Published:2024-03-03

摘要:

为了探索安农1687对于条锈病抗性的遗传机理,加快安农1687小麦品种的推广,为小麦新品种的遗传改良提供参考,减少小麦条锈病对于我国小麦生产安全的危害。以安农1687(安农1687是由安农1106和西农822杂交选育的,对生理小种CYR32具有抗性的半冬性小麦品种)及其姊妹系和亲本为材料,利用小麦55K SNP芯片对安农1687及其双亲和姊妹系进行全基因组扫描,同时利用生理小种CYR32对安农1687及其姊妹系进行接种和鉴定,并在成株期统计其抗条锈病等级,综合接种及田间表型对小麦品系抗条锈病进行评价。鉴定结果显示,安农1687和5个姊妹系(系24、系26、系28、系30、系140)为中抗条锈病,2个姊妹系(系89和系105)为中感条锈病。利用安农1687及其姊妹系间的基因组差异信息,发现差异SNP位点富集在2A染色体短臂的31~37 Mb区间上,说明2A染色体可能存在某个或某些基因导致了这样的结果。为了排除其他抗条锈病基因的干扰,对亲本及2A染色体上携带的已知抗条锈病基因进行验证,结果表明,安农1687的2A染色体短臂的31~37 Mb区间可能存在一个新抗条锈病基因。通过试验和生物信息学分析发现,该区段内的TraesCS2A01G070700基因在抗感品系的NBS结构域上存在差异,感病品系中NBS结构域有3个错义突变,推测TraesCS2A01G070700为安农1687抗条锈病候选基因。

关键词: 小麦, 条锈病, 姊妹系, SNP

Abstract:

To investigate the genetic mechanism behind Annong 1687's ability to resist stripe rust,expedite the dissemination of Annong 1687 wheat variety,offer guidance for enhancing the genetics of novel wheat varieties,and mitigate the damage inflicted by wheat stripe rust on Chinese wheat production.This study employed Annong 1687(Annong 1687 is a semi-winter wheat type with resistance to the less significant physiological species CYR32.It was developed through the crossing of Annong 1106 and Xinong 822)with its sister lines,and parents.The wheat 55K SNP microarray was used to scan the entire genome of Annong 1687 as well as parents and sister lines.Concurrently,Annong 1687 and sister lines were inoculated and characterized employing the physiological race CYR32.The resistance to stripe rust grades was noted at the adult stage of the plant,and the resulting combined inoculation and field phenotypes were evaluated for stripe rust resistance of the wheat lines.The study revealed that Annong 1687 and five sister lines (lines 24,26,28,30 and 140) exhibited moderate resistance to stripe rust,while two sister lines (lines 89 and 105) displayed moderate susceptibility to this disease.Based on genomic differential data comparing Annong 1687 to its sister strains,there was evidence of enriched differential SNP loci in the 31—37 Mb segment of chromosome 2A's short arm,indicating possible presence of a gene or genes responsible for the result.To eliminate any potential interference from other stripe rust resistance genes,we verified the known stripe rust resistance genes present on the parental and chromosome 2A.Our findings indicated the possibility of a new stripe rust resistance gene in the 31—37 Mb interval of the short arm of chromosome 2A in Annong 1687.Experimental and bioinformatic analyses demonstrated that the TraesCS2A01G070700 gene exhibited disparity in the NBS structural domain of resistant and susceptible lines within this interval.Additionally,susceptible lines possessed three missense mutations within the NBS structural domain.It was hypothesised that TraesCS2A01G070700 represented a candidate gene for resistance to stripe rust in Annong 1687.

Key words: Wheat, Stripe rust, Sister lines, Single nucleotide polymorphism microarray

引用本文

李凯丽, 耿明状, 王胜, 郝维浩, 卢杰, 陈璨, 司红起. 安农1687抗条锈病候选基因TraesCS2A01G070700鉴定与分析[J]. 华北农学报, 2024, 39(1): 150-155. doi: 10.7668/hbnxb.20194425.

LI Kaili, GENG Mingzhuang, WANG Sheng, HAO Weihao, LU Jie, CHEN Can, SI Hongqi. Identification and Analysis on the Resistance to Stripe Rust Candidate Gene of TraesCS2A01G070700 from Wheat Cultivar Annong 1687[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(1): 150-155. doi: 10.7668/hbnxb.20194425.

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