摘要： 为了筛选出优异的扁蓿豆育种新材料，本研究采用AFLP和SSR分子标记技术对来自于中国7个省市自治区的15份扁蓿豆种质资源进行遗传多样性的比较分析，结果表明：18对SSR引物扩增出109个多态位点，8个AFLP引物组合扩增出640条带，其中472条多态带。AFLP标记的平均Nei's遗传多样性指数、Shannon多样性指数和遗传分化系数均高于SSR标记。15份扁蓿豆种质的遗传距离和遗传相似系数与地理类群很接近。AFLP和SSR数据的聚类分析显示：15份扁蓿豆种质分为4大类，但是聚类结果与地理类群不完全相符，主成分结果与聚类结果相似，Mantel检测表明：AFLP和SSR数据有较高的显著相关性，AFLP和SSR标记能够有效地对扁蓿豆进行遗传多样性分析，其结果为扁蓿豆育种和资源保护具有指导意义。

关键词: 扁蓿豆, 遗传多样性, AFLP, SSR

Abstract: In order to select new breeding materials of Ruthenia Medic with outstanding.In this study,we used AFLP and SSR markers to investigate the genetic diversity in 15 wild Ruthenia Medic accessions from Seven provinces and autonomous regions of China.A total of 109 alleles were detected using 18 pairs of SSR primers,and 640 bands containing 472 polymorphic bands were revealed using 8 pairs of AFLP primers.The mean Nei's gene diversity,Shannon's information index and genetic differentiation coefficient obtained by AFLP were higher than those obtained by SSR.The genetic distances and similarities among the 15 wild accessions demonstrated their close phylogenetic relationship.Dendrograms with arithmetic average based on both AFLP and SSR data clustering showed that the 15 accessions could be grouped into four main clusters.However,the order of clustering did not completely agree with the geographical distribution.A similar clustering pattern was obtained using principal coordinate analysis.The Mantel test identified a highly significant correlation of the AFLP and SSR data.Thus,combined AFLP and SSR more efficiently estimated and validated the genetic diversity of the Ruthenia Medic accessions.These results can aid the formulation of breeding and germplasm conservation strategies for Ruthenia Medic.

Key words: M. ruthenica, Genetic diversity, AFLP, SSR

中图分类号: 

• S52.03

• Q78