利用叶绿体基因组SSR标记揭示薏苡属种质资源的遗传多样性

doi:10.7668/hbnxb.20190745

摘要/Abstract

摘要： 从分子水平研究国内外薏苡属种质资源的遗传多样性差异，为薏苡属种质资源的保护、发掘和利用提供依据。从38对cpSSR引物中筛选出33对多态性引物，对93份薏苡属资源的遗传多样性、群体结构和亲缘关系进行了比较分析。33对cpSSR共扩增出153个等位基因，每个位点等位基因数目3～8个，平均4.64个，Nei's多样性指数（H）和Shannon's信息指数（I）平均为0.368 8和0.720 2，种质资源的遗传多样性丰富。野生和栽培类群之间遗传分化不明显，北方早熟生态型、长江中下游中熟生态型和南方晚熟生态型类群之间的遗传距离相对较近、一致度高，但是与国外类型的遗传差异大。小珠薏苡种和窄果薏苡种独立分支，表现出明显的分化差异；台湾薏苡变种、念珠薏苡变种、薏苡变种和薏米变种4个类群之间的遗传差异较小；种质遗传距离与地理距离不存在绝对相关性，而不同类群之间可能存在基因渐渗现象。

关键词: cpSSR标记, 遗传多样性, 种质资源, 薏苡属

Abstract: For providing a solid base for germplasm protection, discovering and genetic development in Coix L. that collected from China and abroad,whereas genetic diversity and differentiation in Coix L. were assessed. Thirty-three of 38 polymorphic cpSSR primers were amplified in 93 germplasm resources and then their genetic diversity, relationship and structure among different populations were compared. It was reveled that 153 alleles had been amplified from 33 primers, it was observed 3 to 8 alleles with an average of 4.64(Na). The means of Nei's gene diversity index(H) and Shannon's information index(I) were 0.368 8 and 0.720 2, respectively, showing rich diversity in tested materials. It was also revealed that wild and cultivar populations had no obvious genetic divergence, and three ecotype populations of EEMC, EMMCYRR and ELMSC also clustered together that showing closer genetic distance and higher genetic identity, while foreign type(FT) population were more different to other three groups. C. puellarum Balansa and C. stenocarpa Balansa populations divided alone respectively indicating striking genetic divergence; and whereas there were less genetic distance among other four varieties of C. chinensis var. formosana(Ohwi) L. Liu, C. lacryma-jobi var. maxima Makino, C. lacryma-jobi var. lacryma-jobi and C. chinensis var. chinesis Tod.. No absolutely relation between genetic distance and geographic location were observed by clustering analysis, suggesting introgression among different populations.

Key words: cpSSR marker, Genetic diversity, Germplasm, Coix L.

中图分类号:

李祥栋, 石明, 陆秀娟, 潘虹, 魏心元, 陆平. 利用叶绿体基因组SSR标记揭示薏苡属种质资源的遗传多样性[J]. 华北农学报, 2019, 34(S1): 6-14. doi: 10.7668/hbnxb.20190745.

LI Xiangdong, SHI Ming, LU Xiujuan, PAN Hong, WEI Xinyuan, LU Ping. Genetic Diversity of Germplasm Resources in Job's Tears Revealed by Chloroplast Genome SSR Markers[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(S1): 6-14. doi: 10.7668/hbnxb.20190745.

http://www.hbnxb.net/CN/Y2019/V34/IS1/6

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