摘要： 发掘优异耐盐种质是进行豇豆品种耐盐性遗传改良的基础。以蛭石为培养基质，在子叶展开期，以分别添加50，100，150，200，250 mmol/L NaCl的1/2 Hoagland营养液对12个豇豆品种进行盐胁迫处理，研究不同盐浓度胁迫对豇豆耐盐等级（STR）、叶绿素含量（SPAD）、株高（PH）、根长（RL）、地上部鲜质量（AFW）、地上部干质量（ADW）、根鲜质量（RFW）和根干质量（RDW）等8个指标的影响，鉴定适宜盐浓度；依据隶属函数值（F_i），综合评价12个品种的耐盐性，并确定耐盐鉴定方法；根据此方法，评价84份豇豆种质资源的耐盐性。结果表明：随着盐浓度增加，12个品种STR值呈上升趋势，150 mmol/L NaCl胁迫下变异系数最大，确定150 mmol/L为豇豆苗期耐盐性鉴定适宜浓度；筛选出耐盐品种苏紫41和绿领四号（F_i>0.60），盐敏感品种苏豇1419（F_i<0.30）；建立了一种以蛭石为基质，150 mmol/L NaCl处理4周左右进行豇豆苗期耐盐性鉴定的方法；84份豇豆种质资源耐盐性F_i变幅为0.06~0.88，遴选出苏紫豇1号和紫筋肉豆角等8份耐盐种质资源。本研究筛选到10份耐盐种质资源（F_i>0.60），为豇豆耐盐遗传改良提供参考。

关键词: 豇豆, 耐盐性, 隶属函数, 种质资源

Abstract: Screening salt-tolerant yardlong bean is very important for developing salt resistant cultivars. This work was performed on 12 varieties of yardlong bean that have been cultivated across large areas in Jiangsu Province. Vermiculite was used as a cultivation matrix. During the cotyledon expansion stage, 50, 100, 150, 200, 250 mmol/L of NaCl solution was added to 1/2 Hoagland nutrient solution for treatment and the 1/2 Hoagland nutrient solution served as a control. After 4 weeks of treatment, the effects of eight indicators including salt tolerance rating(STR), chlorophyll content(SPAD), plant height(PH), root length(RL), aerial biomass fresh weight(AFW), aerial biomass dry weight(ADW), root fresh weight(RFW), and root dry weight(RDW)of yardlong beans under different concentration of salt stresses during the seedling stage were studied. The salt tolerance of 12 varieties was evaluated by membership function value(F_i), and the evaluation method of tolerance to salt in yardlong bean was determined. Based on the above method, we also assessed salt-tolerance germplasm resources in 84 representative yardlong bean accessions at the seedling stage. It was found that the STR value of 12 varieties increased with the increase of salt concentration, and the coefficient of variation was the highest under 150 mmol/L NaCl stress. Thus, we choose it as the optimum concentration for evaluating the salt tolerance of 12 yardlong bean varieties by membership function value(F_i). Suzi 41 and Lüling 4 were selected as salt-tolerant varieties(F_i>0.60), and Sujiang 1419 as salt-sensitive variety(F_i<0.30). The evaluation method of tolerance to salt was established in yardlong bean seedling stage, using the seedlings grown in vermiculite with 150 mmol/L NaCl treatment for about four weeks. There was a large variation of 84 yardlon bean germplasm accessions in F_i, 0.06-0.88, and Suzijing 1 and Zijinroudoujiao etc. as the 8 salt-tolerance accessions were selected. In this study, 10 salt-tolerant germplasm accessions screened will provide gene resources for the breeding of salt-tolerant yardlong bean.

Key words: Yardlong bean, Salt tolerance, Membership function, Germplasm

中图分类号: 

• S643.01