江苏大豆上分离的豇豆轻斑驳病毒基因组序列克隆及特征分析

doi:10.7668/hbnxb.20193080

华北农学报 ›› 2022, Vol. 37 ›› Issue (3): 200-205. doi: 10.7668/hbnxb.20193080

所属专题： 油料作物；植物保护；生物技术；

• 资源环境·植物保护 • 上一篇下一篇

江苏大豆上分离的豇豆轻斑驳病毒基因组序列克隆及特征分析

吉颖¹, 孙枫², 吴淑华², 李硕², 涂丽琴², 高丹娜², 崔晓艳³, 陈新³, 季英华²^,, 郭青云¹^,

1.青海大学农林科学院,青海省农业有害生物综合治理重点实验室,农业部西宁作物有害生物科学观测实验站,青海西宁 810016
2.江苏省农业科学院植物保护研究所,江苏南京 210014
3.江苏省农业科学院经济作物研究所,江苏南京 210014

收稿日期:2022-03-28 出版日期:2022-06-30
通讯作者: 季英华, 郭青云
作者简介:
吉颖(1997-),男,江西赣州人,在读硕士,主要从事园艺蔬菜作物病毒研究。
基金资助:
国家自然科学基金项目(32072506); 国家现代农业产业技术体系建设专项(CARS-24-C-01); 国家现代农业产业技术体系建设专项(1011); 青海省农业有害生物综合治理重点实验室(2021-ZJ-Y08)

Genomic Sequence Cloning and the Characteristics Analysis of Cowpea mild mottle virus Infecting Soybean from Jiangsu Province

JI Ying¹, SUN Feng², WU Shuhua², LI Shuo², TU Liqin², GAO Danna², CUI Xiaoyan³, CHEN Xin³, JI Yinghua²^,, GUO Qingyun¹^,

1. Academy of Agriculture and Forestry Sciences,Qinghai University,Qinghai Provincial Key Laboratory of Agricultural Integrated Pest Management,Scientific Observing and Experimental Station of Crop Pests in Xining,Ministry of Agriculture and Rural Affairs,Xining 810016,China
2. Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China
3. Institute of Crop Economics Research, Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China

Received:2022-03-28 Published:2022-06-30
Contact: JI Yinghua, GUO Qingyun

摘要/Abstract

摘要：

为明确豇豆轻斑驳病毒江苏分离物的基因组结构特征,阐明其分类地位及进化特点,选择采自江苏的CpMMV大豆分离物作为研究对象,针对病毒基因组序列设计了4对特异性引物,以病样总RNA反转录后获得的cDNA为模板,通过分段法对其基因组序列片段进行了PCR扩增,扩增产物克隆至T载体经验证后进行序列测定,获得的序列片段经拼接组装得到病毒全基因组序列。序列分析结果显示,CpMMV江苏分离物基因组核苷酸序列全长8 194 bp,编码6个蛋白,5'端和3'端各含有一个非编码区(UTR),长度分别为72,117 nt;在编码的6个蛋白中,CP与其他分离物之间的同源性较高(96.5%~100.0%),相对保守;而RdRp(81.1%~98.2%)、TGB1(81.0%~97.0%)、TGB3(80.9%~95.6%)同源性相对较低,在不同分离物中表现较好的多样性;基于全基因组序列的系统进化分析结果显示,江苏分离物与已公开的其他CpMMV分离物同源性较高,共同聚类到一个大分支上,其中与中国安徽分离物同源性最高(98.2%),与海南分离物次之(96.0%),而与美国、巴西、印度、墨西哥、肯尼亚等国外分离物同源性相对较低(<82.7%)。这些研究结果表明,CpMMV江苏分离物与其他CpMMV分离物具有类似的基因组结构特征,在系统进化上,江苏分离物与国内分离物近缘而与国外分离物远缘。

关键词: 大豆, 豇豆轻斑驳病毒, 全基因组序列, 克隆, 特征分析

Abstract:

To characterize the genomic structure of Cowpea mild mottle virus (CpMMV)Jiangsu isolate and clarify its taxonomic status and evolutionary characteristics,4 pairs of specific primers were designed and the genomic fragments were amplified using cDNA obtained from reverse transcription of total RNA of a soybean sample collected from Jiangsu as a temple.The amplified products were cloned and sequenced,and the obtained fragments were assembled to get the full genomic sequence.Sequence analysis revealed the genome of Jiangsu isolate were 8 194 bp in length,containing 6 ORFs and 2 untranslated regions(UTRs):5' UTR(72 nt)and 3' UTR(117 nt).Among the six proteins encoded by CpMMV,CP shared highest amino acid sequence identities with other isolates(96.5%-100.0%),while RdRp(81.1%-98.2%),TGB1(81.0%-97.0%)and TGB3(80.9%-95.6%)shared relatively low sequence identities,indicating CP might be a conservative gene and RdRp,TGB1,TGB3 might show good diversity in different isolates.The phylogenetic analysis based on the whole genomic sequence revealed that Jiangsu isolate was clustered with other CpMMV isolates in a branch and it shared highest nucleotide sequence identities with 2 Chinese isolates(98.2% with Anhui isolate,96.0% with Hainan isolate),compared with other foreign isolates such as the United States,Brazil,India,Mexico,Kenya and so on(<82.7%).These results clarified the genomic structure of Jiangsu isolate,which shared similar character with other CpMMV isolates and phylogenetic analysis revealed Jiangsu isolates clustered with other isolates from China with higher sequences identities,compared with foreign isolates.

Key words: Soybean, Cowpea mild mottle virus, Genomic sequence, Clone, Characteristics analysis

吉颖, 孙枫, 吴淑华, 李硕, 涂丽琴, 高丹娜, 崔晓艳, 陈新, 季英华, 郭青云. 江苏大豆上分离的豇豆轻斑驳病毒基因组序列克隆及特征分析[J]. 华北农学报, 2022, 37(3): 200-205. doi: 10.7668/hbnxb.20193080.

JI Ying, SUN Feng, WU Shuhua, LI Shuo, TU Liqin, GAO Danna, CUI Xiaoyan, CHEN Xin, JI Yinghua, GUO Qingyun. Genomic Sequence Cloning and the Characteristics Analysis of Cowpea mild mottle virus Infecting Soybean from Jiangsu Province[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(3): 200-205. doi: 10.7668/hbnxb.20193080.

http://www.hbnxb.net/CN/Y2022/V37/I3/200

图/表 7

参考文献 24

[1]	Naidu R A, Gowda S, Satyanarayana T, Boyko V, Reddy A S, Dawson W O, Reddy D V. Evidence that whitefly-transmitted Cowpea mild mottle virus belongs to the genus Carlavirus[J]. Archives of Virology, 1998, 143(4):769-780.doi: 10.1007/s007050050328. doi: 10.1007/s007050050328 pmid: 9638146
[2]	Menzel W, Winter S, Vetten H J. Complete nucleotide sequence of the type isolate of Cowpea mild mottle virus from Ghana[J]. Archives of Virology, 2010, 155(12):2069-2073.doi: 10.1007/s00705-010-0821-y. doi: 10.1007/s00705-010-0821-y pmid: 20890716
[3]	Muniyappa V. Transmission of Cowpea mild mottle virus by Bemisia tabaciin a nonpersistent manner[J]. Plant Disease, 1983, 67(4):391.doi: 10.1094/pd-67-391. doi: 10.1094/pd-67-391 URL
[4]	Zanardo L G, Carvalho C M. Cowpea mild mottle virus (Carlavirus,Betaflexiviridae):A review[J]. Tropical Plant Pathology, 2017, 42(6):417-430.doi: 10.1007/s40858-017-0168-y. doi: 10.1007/s40858-017-0168-y URL
[5]	Brunt A A, Kenten R H. Cowpea mild mottle,a newly recognized virus infecting cowpeas(Vigna unguiculata)in Ghana[J]. The Annals of Applied Biology, 1973, 74(1):67-74.doi: 10.1111/j.1744-7348.1973.tb07723.x. doi: 10.1111/j.1744-7348.1973.tb07723.x. URL
[6]	Thouvenel J C. Isolation of Cowpea mild mottle virus from diseased soybeans in the Ivory coast[J]. Plant Disease, 1982, 66(1):336.doi: 10.1094/pd-66-336. doi: 10.1094/pd-66-336 URL
[7]	Orawu M, Obuo J, Omadi R. Distribution and detection of cowpea viruses infecting cowpea in Uganda[J]. American Journal of Plant Sciences, 2015, 6(5):574-581.doi: 10.4236/ajps.2015.65062. doi: 10.4236/ajps.2015.65062 URL
[8]	Odhiambo Patrick O, Were H, Ndonga M, Benard M. First report of Cowpea polero virus 1 (CPPV1)infecting cowpea in Kenya[J]. International Journal of Genetics and Genomics, 2019, 7(4):119-123.doi: 10.11648/j.ijgg.20190704.16. doi: 10.11648/j.ijgg.20190704.16 URL
[9]	Zinsou V A, Afouda L A C, Zoumarou-Wallis N, Dossou L, Gomez J, Soumaïla F, Afloukou F, Kotchofa R. Importance of Cowpea mild mottle virus on soybean(Glycine max)in Benin and effect of planting date on Soybean (G.max)virus level in Northern Benin[J]. Crop Protection, 2015, 72:139-143.doi: 10.1016/j.cropro.2015.03.006. doi: 10.1016/j.cropro.2015.03.006 URL
[10]	Brito M, Fernandez-Rodríguez T, Garrido M J, Mejías A, Romano M, Marys E. First report of Cowpea mild mottle Carlavirus on yardlong bean(Vigna unguiculata subsp.sesquipedalis)in Venezuela[J]. Viruses, 2012, 4(12):3804-3811.doi: 10.3390/v4123804. doi: 10.3390/v4123804 URL
[11]	Pardina P E R, Arneodo J D, Truol G A, Herrera P S, Laguna I G. First record of Cowpea mild mottle virus in bean crops in Argentina[J]. Australasian Plant Pathology, 2004, 33(1):129.doi: 10.1071/ap03076. doi: 10.1071/ap03076 URL
[12]	Zanardo L G, Silva F N, Lima A T M, Milanesi D F, Castilho-Urquiza G P, Almeida A M R, Zerbini F M, Carvalho C M. Molecular variability of Cowpea mild mottle virus infecting soybean in Brazil[J]. Archives of Virology, 2014, 159(4):727-737.doi: 10.1007/s00705-013-1879-0. doi: 10.1007/s00705-013-1879-0 pmid: 24142270
[13]	Rosario K, Capobianco H, Ng T F F, Breitbart M, Polston J E. RNA viral metagenome of whiteflies leads to the discovery and characterization of a whitefly-transmitted Carlavirus in North America[J]. PLoS One, 2014, 9(1):e86748.doi: 10.1371/journal.pone.0086748. doi: 10.1371/journal.pone.0086748 URL
[14]	Celli M G, Perotto M C, Merino M C, Nome C F D, Flores C R, Conci V C. First report of Cowpea mild mottle virus in chia(Salvia hispanica)[J]. Crop Protection, 2016, 89:1-5.doi: 10.1016/j.cropro.2016.05.014. doi: 10.1016/j.cropro.2016.05.014 URL
[15]	Chiquito-Almanza E, Caballero-Pérez J, Guevara-Olvera L, Acosta-García G, Pérez-Pérez M C I, Acosta-Gallegos J A, Anaya-López J L. First report of Cowpea mild mottle virus infecting cultivated and wild Phaseolus in the Central-Western Region of Mexico[J]. Plant Disease, 2018, 102(5):1047.doi: 10.1094/pdis-07-17-0990-pdn. doi: 10.1094/PDIS-07-17-0990-PDN
[16]	Iwaki M. Whitefly transmission and some properties of Cowpea mild mottle virus on soybean in Thailand[J]. Plant Disease, 1982, 66(1):365.doi: 10.1094/pd-66-365. doi: 10.1094/pd-66-365 URL
[17]	Baranwal V K, Jain P, Saritha R K, Jain R K, Gautam N K. Detection and partial characterization of Cowpea mild mottle virus in mungbean and urdbean by deep sequencing and RT-PCR[J]. Crop Protection, 2015, 75:77-79.doi: 10.1016/j.cropro.2015.05.015. doi: 10.1016/j.cropro.2015.05.015 URL
[18]	Tavassoli M, Shahraeen N, Ghorbani S. Detection and some properties of Cowpea mild mottle virus isolated from soybean in Iran[J]. Pakistan Journal of Biological Sciences, 2008, 11(23):2624-2628.doi: 10.3923/pjbs.2008.2624.2628. doi: 10.3923/pjbs.2008.2624.2628 URL
[19]	Chang C A, Chien L Y, Tsai C F, Lin Y Y, Cheng Y H. First report of Cowpea mild mottle virus in Cowpea and French Bean in Taiwan[J]. Plant Disease, 2013, 97(7):1001.doi: 10.1094/PDIS-10-12-0935-PDN. doi: 10.1094/PDIS-10-12-0935-PDN pmid: 30722532
[20]	杨晓, 张宇, 陈莎, 李桑桑, 龚一诺, 张卓, 鲁清华, 胡荣, 刘勇, 张德咏. 豇豆轻斑驳病毒海南分离物全基因组序列测定及分子特征[J]. 中国蔬菜, 2019(6):35-38.
	Yang X, Zhang Y, Chen S, Li S S, Gong Y N, Zhang Z, Lu Q H, Hu R, Liu Y, Zhang D Y. Genomic sequences measure and molecular characteristics of Cowpea mild mottle virus of Hainan isolate[J]. China Vegetables, 2019(6):35-38.
[21]	Wei Z Y, Wu G W, Ye Z X, Jiang C, Mao C Y, Zhang H H, Miao R P, Yan F, Li J M, Chen J P, Sun Z T. First report of Cowpea mild mottle virus infecting soybean in China[J]. Plant Disease, 2020, 104(9):2534.doi: 10.1094/pdis-01-20-0063-pdn. doi: 10.1094/pdis-01-20-0063-pdn
[22]	吴淑华, 赵文浩, 李廷芳, 程兆榜, 潘宝贵, 郭青云, 王述彬, 季英华, 周益军. 南京辣椒上一种斑驳类型病毒病的分子鉴定[J]. 江苏农业学报, 2015, 31(6):1284-1290.doi: 10.3969/j.issn.1000-4440.2015.06.014. doi: 10.3969/j.issn.1000-4440.2015.06.014
	Wu S H, Zhao W H, Li T F, Cheng Z B, Pan B G, Guo Q Y, Wang S B, Ji Y H, Zhou Y J. Molecular identification of a virus causing mottle symptoms in pepper leaves in Nanjing[J]. Jiangsu Journal of Agricultural Sciences, 2015, 31(6):1284-1290.
[23]	Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5:molecular evolutionary genetics analysis using maximum likelihood,evolutionary distance,and maximum parsimony methods[J]. Molecular Biology and Evolution, 2011, 28(10):2731-2739.doi: 10.1093/molbev/msr121. doi: 10.1093/molbev/msr121 URL
[24]	Almeida Á M R, Piuga F F, Marin S R R, Kitajima E W, Gaspar J O, de Oliveira T G, de Moraes T G. Detection and partial characterization of a Carlavirus causing stem necrosis of soybean in Brazil[J]. Fitopatologia Brasileira, 2005, 30(2):191-194.doi: 10.1590/s0100-41582005000200016. doi: 10.1590/s0100-41582005000200016 URL

引物 Primer	序列(5'-3') Sequence(5'-3')	退火温度/℃ Tm	片段/bp Fragment
CpMM-1F	GAAAAACAGAGCAACAATATCTAGC	53.60	2 355
CpMM-1R	CTTCATGCTTTCCACATCTTCC	52.92
CpMM-2F	GATATTGCCTGACAGGTCTGAT	52.70	2 568
CpMM-2R	TGATTCTCAATAGTGGCTGCAG	54.80
CpMM-3F	TCTCCAAGCCTCACGTTGAG	54.74	2 141
CpMM-3R	TATTGCAGACATAACAGACTCTTG	53.33
CpMM-4F	TCAACACAGRTTCCCTTTTGG	51.05	1 701
CpMM-4R	AAAACCAGGAAAATAACWTTAAAACC	50.22

引物 Primer	序列(5'-3') Sequence(5'-3')	退火温度/℃ Tm	片段/bp Fragment
CpMM-1F	GAAAAACAGAGCAACAATATCTAGC	53.60	2 355
CpMM-1R	CTTCATGCTTTCCACATCTTCC	52.92
CpMM-2F	GATATTGCCTGACAGGTCTGAT	52.70	2 568
CpMM-2R	TGATTCTCAATAGTGGCTGCAG	54.80
CpMM-3F	TCTCCAAGCCTCACGTTGAG	54.74	2 141
CpMM-3R	TATTGCAGACATAACAGACTCTTG	53.33
CpMM-4F	TCAACACAGRTTCCCTTTTGG	51.05	1 701
CpMM-4R	AAAACCAGGAAAATAACWTTAAAACC	50.22

登录号 Accession No.	依赖RNA的RNA 聚合酶 RdRp	三重基因块1 TGB1	三重基因块2 TGB2	三重基因块3 TGB3	外壳蛋白 CP	核酸结合蛋白 NBP
MN908944	98.2	97.0	99.1	95.6	100.0	98.1
KY420906	96.4	93.1	97.2	95.6	100.0	93.2
KC774019	86.2	86.6	87.8	82.4	98.3	92.2
KC774020	86.2	86.1	91.5	82.4	98.6	93.2
MT473963	85.9	87.0	92.5	85.3	98.6	91.3
KC884244	85.4	86.6	90.6	80.9	97.9	92.2
KC884245	86.0	86.1	91.5	83.8	98.3	92.2
KC884246	85.2	86.1	92.5	83.8	97.6	91.3
KC884247	81.3	86.1	92.5	83.8	97.6	91.3
KC884249	83.5	86.6	92.5	82.4	98.3	93.2
MK069989	86.1	85.7	91.5	82.4	98.6	94.2
MK984605	86.3	86.1	90.6	83.8	96.5	87.4
KC884248	85.2	81.4	84.0	82.4	98.3	87.4
MK202583	85.9	81.0	85.8	82.4	99.3	91.3
MH345698	81.1	83.5	86.8	83.8	98.6	89.3

登录号 Accession No.	依赖RNA的RNA 聚合酶 RdRp	三重基因块1 TGB1	三重基因块2 TGB2	三重基因块3 TGB3	外壳蛋白 CP	核酸结合蛋白 NBP
MN908944	98.2	97.0	99.1	95.6	100.0	98.1
KY420906	96.4	93.1	97.2	95.6	100.0	93.2
KC774019	86.2	86.6	87.8	82.4	98.3	92.2
KC774020	86.2	86.1	91.5	82.4	98.6	93.2
MT473963	85.9	87.0	92.5	85.3	98.6	91.3
KC884244	85.4	86.6	90.6	80.9	97.9	92.2
KC884245	86.0	86.1	91.5	83.8	98.3	92.2
KC884246	85.2	86.1	92.5	83.8	97.6	91.3
KC884247	81.3	86.1	92.5	83.8	97.6	91.3
KC884249	83.5	86.6	92.5	82.4	98.3	93.2
MK069989	86.1	85.7	91.5	82.4	98.6	94.2
MK984605	86.3	86.1	90.6	83.8	96.5	87.4
KC884248	85.2	81.4	84.0	82.4	98.3	87.4
MK202583	85.9	81.0	85.8	82.4	99.3	91.3
MH345698	81.1	83.5	86.8	83.8	98.6	89.3

登录号 Accession No.	长度/nt Length	病毒名称 Virus	寄主 Host	分离物 Isolate	国家/地区 Country/Region	同源性/% Identities
MN908944	8 256	豇豆轻斑驳病毒	大豆	Anhui_SZ_DN1383	中国/安徽	98.2
KY420906	8 193	豇豆轻斑驳病毒	豇豆	Hainan 1	中国/海南	96.0
KC774019	8 207	豇豆轻斑驳病毒	烟粉虱	Florida_Whiteflies_2007	美国	82.7
KC774020	8 209	豇豆轻斑驳病毒	菜豆	Florida_Bean_2011	美国	82.7
MT473963	8 195	豇豆轻斑驳病毒	大豆	Casa Branca_BR	巴西	82.6
KC884244	8 196	豇豆轻斑驳病毒	大豆	CPMMV:BR:MG:09:2	巴西	82.5
KC884245	8 196	豇豆轻斑驳病毒	大豆	CPMMV:BR:MG:09:3	巴西	82.6
KC884246	8 180	豇豆轻斑驳病毒	大豆	CPMMV:BR:MT:02:1	巴西	82.6
KC884247	8 191	豇豆轻斑驳病毒	大豆	CPMMV:BR:BA:02	巴西	81.7
KC884249	8 194	豇豆轻斑驳病毒	大豆	CPMMV:BR:GO:10:5	巴西	82.2
MK069989	8 191	豇豆轻斑驳病毒	菜豆	CN2	墨西哥	82.5
MK984605	8 196	豇豆轻斑驳病毒	番木瓜	KE-Kit_01	肯尼亚	82.6
KC884248	8 198	豇豆轻斑驳病毒	大豆	CPMMV:BR:GO:01:1	巴西	80.3
MK202583	8 194	豇豆轻斑驳病毒	菜豆	CPMMV:BR:GO:14	巴西	80.9
MH345698	8 189	豇豆轻斑驳病毒	黑吉豆	CpMMV-Urd-Kanpur	印度	76.6
HQ184471	8 127	豇豆轻斑驳病毒	豇豆	Ghana	加纳	64.7
NC_011525	7 890	马铃薯潜隐病毒	马铃薯		加拿大	54.0
NC_001361	8 533	马铃薯M病毒	番茄	Russian wild	俄罗斯	51.5
NC_043081	5 218	黄瓜脉明病毒	西瓜	PV-0985	坦桑尼亚	60.8
NC_007289	8 478	马铃薯S病毒	马铃薯	Leona	德国	49.9
NC_006550	9 104	甘薯褪绿斑点病毒	甘薯	Hoima 4	乌干达	49.3

选择文件类型/文献管理软件名称

选择包含的内容

江苏大豆上分离的豇豆轻斑驳病毒基因组序列克隆及特征分析

Genomic Sequence Cloning and the Characteristics Analysis of Cowpea mild mottle virus Infecting Soybean from Jiangsu Province

RichHTML

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 24

相关文章 15

编辑推荐

Metrics

本文评价

[1]	肖士奎, 李芳, 张文婷, 吕淑芳, 史国安, 吴疆, 范丙友. *芍药ACS基因的克隆及其蛋白质原核表达*[J]. 华北农学报, 2022, 37(5): 36-44.
[2]	侯瑞泽, 侯玉翔, 许小勇, 李璿, 李梅兰. 普通白菜CYP同源基因克隆与表达分析[J]. 华北农学报, 2022, 37(5): 45-51.
[3]	金一锋, 高岩松, 王琦, 王萌萌, 赵迪, 熊毅, 陈阳. *草地早熟禾蛋白激酶SnRK2.4* 基因克隆及非生物胁迫响应分析**[J]. 华北农学报, 2022, 37(5): 9-15.
[4]	张冬梅, 冯亚艳, 修志君, 杨春芳, 杜美娥, 李得宙, 张笑宇. *硅酸钠诱导的马铃薯抗黑痣病StWRKY11基因克隆及生物信息学分析*[J]. 华北农学报, 2022, 37(5): 194-203.
[5]	郑玉斯, 王培, 郭颖, 白丽蓉, 喻达辉, 赵森. *合浦珠母贝PfCLEC17A* 基因的克隆及表达分析**[J]. 华北农学报, 2022, 37(5): 230-238.
[6]	贾建平, 李金鸿, 于敬文, 余曦玥, 彭德良, 李惠霞, 黄文坤. 氮肥对控制大豆孢囊线虫病危害的影响[J]. 华北农学报, 2022, 37(4): 198-205.
[7]	张斌. *大豆GmPP2C89基因在盐胁迫中的功能分析*[J]. 华北农学报, 2022, 37(4): 20-27.
[8]	吉祥, 宋志成, 韦晓玲, 杨煜, 隋炯明, 郭宝太. 马铃薯卷叶病毒与S病毒重组双CP多克隆抗体的制备[J]. 华北农学报, 2022, 37(4): 212-219.
[9]	王亚男, 梁岩岩, 严文培, 张银萍, 李强, 吴秀菊. *北细辛AhOMT1基因的克隆及原核表达分析*[J]. 华北农学报, 2022, 37(4): 62-70.
[10]	程春红, 游经番, 蔡兆明, 叶春宏, 陈丽. *茎瘤芥BjuEAR1-1的克隆与表达分析*[J]. 华北农学报, 2022, 37(3): 37-43.
[11]	唐忠丽, 逯晓楠, 赵蕊, 刘庆华, 李梅兰, 雷逢进, 许小勇. *黄皮西葫芦类胡萝卜素合成酶基因的鉴定及PSY1和LCYE2克隆分析*[J]. 华北农学报, 2022, 37(3): 60-67.
[12]	李艳燕, 赵彩桐, 齐阳阳, 刘明, 张书瑞, 刘志华, 李文滨, 姜振峰. 大豆主茎木质素积累规律分析[J]. 华北农学报, 2022, 37(3): 77-85.
[13]	张晋玉, 徐新娟, 晁毛妮, 张晓红, 吴向远, 高际涛, 黄中文. *大豆GmZAT12基因的克隆和表达分析*[J]. 华北农学报, 2022, 37(3): 1-7.
[14]	贾志强, 许云玉, 高雪, 陶宏征, 陈增敏, 刘雅婷, 李永忠. *辣椒CaWRKY30转录因子的克隆、亚细胞定位及番茄斑萎病毒侵染下的表达分析*[J]. 华北农学报, 2022, 37(3): 186-192.
[15]	景凡丽, 张沛沛, 苗永平, 陈涛, 刘媛, 杨德龙. *小麦TaSPP基因的克隆及表达分析*[J]. 华北农学报, 2022, 37(2): 28-34.

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

江苏大豆上分离的豇豆轻斑驳病毒基因组序列克隆及特征分析

Genomic Sequence Cloning and the Characteristics Analysis of Cowpea mild mottle virus Infecting Soybean from Jiangsu Province

RichHTML

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 24

相关文章 15

编辑推荐

Metrics

本文评价