摘要： 为研究盐胁迫对甜荞生理特性及FtNHX1基因表达的影响，以12个甜荞新品种（平荞2号、定甜荞3号、赤甜荞1号、TQ11-3、T407-8、通荞2号、云甜荞1号、蒙0825、甜荞1307-179、平选03-122、TQ11-6和赤甜荞2号）为试验材料，盐胁迫下通过测定种子发芽率、幼苗鲜质量、根系活力、叶片质膜透性、MDA含量、SOD活性和根部FtNHX1基因表达量等指标，比较盐胁迫下12个甜荞新品种生理特性及FtNHX1基因表达的差异。结果表明，盐胁迫下定甜荞3号和赤甜荞1号种子发芽率及幼苗鲜质量降低幅度较大，而平荞2号和T407-8种子发芽率与对照无显著差异，且盐胁迫对这2个甜荞品种幼苗生长有明显促进效应；定甜荞3号和赤甜荞1号叶片质膜透性和MDA含量增加幅度较大，而平荞2号和T407-8叶片质膜透性与对照无显著差异，且叶片MDA含量增加幅度较小；定甜荞3号和赤甜荞1号根系活力和叶片SOD活性降低幅度较大，而平荞2号和T407-8根系活力降低幅度较小，且叶片SOD活性显著增加，抗逆性强；定甜荞3号根部FtNHX1表达量显著降低，而平荞2号和T407-8根部FtNHX1基因表达量显著增加，耐盐性强。确定平荞2号和T407-8为甜荞耐盐品种，定甜荞3号和赤甜荞1号为盐敏感品种。

关键词: 甜荞, 盐胁迫, 生理特性, FtNHX1基因表达量, 耐盐性

Abstract: In order to study the effects of salt stress on physiological characteristics and relative expression of FtNHX1 in Fagopyrum esculentum,twelve new Fagopyrum esculentum varieties (Pingqiao 2,Dingtianqiao 3,Chitianqiao 1,TQ11-3,T407-8,Tongqiao 2,Yuntianqiao 1,Meng 0825,Tianqiao 1307-179,Pingxuan 03-122,TQ11-6 and Chitianqiao 2) were used as experimental materials. The indexes of the seeds germination rate,seedlings fresh weight,roots vigor,leaf plasmalemma permeability,MDA content,SOD activity and relative expression of FtNHX1 in roots were determined under salt stress to compare the physiological characteristics and relative expression of FtNHX1 among twelve new Fagopyrum esculentum varieties. The results showed that the seeds germination rate and seedlings fresh weight of Dingtianqiao 3 and Chitianqiao 1 were decreased more than that of Pingqiao 2 and T407-8,while the seeds germination rate of Pingqiao 2 and T407-8 had no significant difference with the control,and had obviously promoting effects of salt stress on seedlings growth of the two Fagopyrum esculentum varieties. The leaf plasmalemma permeability and MDA content of Dingtianqiao 3 and Chitianqiao 1 were increased greatly,while the leaf plasmalemma permeability of Pingqiao 2 and T407-8 had no significant difference with the control,and the leaf MDA content of Pingqiao 2 and T407-8 increased to a lesser extent. The roots vigor and leaf SOD activity of Dingtianqiao 3 and Chitianqiao 1 were decreased greatly,while the roots vigors of Pingqiao 2 and T407-8 were decreased little,and the leaf SOD activities of Pingqiao 2 and T407-8 were increased significantly,indicated that had strong stress resistance. The relative expression of FtNHX1 gene of Dingtianqiao 3 was decreased significantly,while that of Pingqiao 2 and T407-8 were increased significantly,indicated that more salt tolerance. Fagopyrum esculentum varieties of Pingqiao 2 and T407-8 were determined salt tolerant varieties,while that of Dingtianqiao 3 and Chitianqiao 1 were determined salt sensitive varieties.

Key words: Fagopyrum esculentum, Salt stress, Physiological characteristics, Relative expression of FtNHX1 gene, Salt tolerance

中图分类号: 

• Q945.78

• Q786