摘要：

通过鉴定红麻全基因组中WRKY基因家族所有成员,并分析其在盐胁迫下的表达特征,从而为研究WRKY基因家族成员在红麻响应盐胁迫过程中的生物学功能奠定坚实基础。利用生物信息学的方法对红麻全基因组中的WRKY基因家族成员进行鉴定,并对WRKY基因家族各成员的理化性质、系统发育和保守功能域进行分析,同时利用实时荧光定量PCR技术分析盐胁迫下WRKY基因家族各成员的表达特征。结果表明,共鉴定出33个WRKY基因家族成员,且该基因家族成员不均匀地分布在12条染色体上,每个基因家族成员的理化性质如氨基酸数目、分子质量、理论等电点都存在一定的差异,且每个基因家族成员的氨基酸保守序列WRKYGQK没有发生变异。红麻和拟南芥的WRKY基因家族系统进化树分析表明,33个WRKY家族成员分成了3个类群,GroupⅠ、GroupⅡ、Group Ⅲ,其中Group Ⅲ包含了5个亚组。实时荧光定量PCR技术分析结果表明,盐胁迫诱导表达的WRKY家族成员有26个,其中23个具有正向调控作用,3个具有负向调控作用。共鉴定出红麻WRKY家族成员33个,其中26个WRKY家族成员参与了红麻盐胁迫响应过程。

关键词: 红麻, WRKY, 生物信息学, 盐胁迫

Abstract:

To provide a solid foundation for studying the biological function of WRKY family members in kenaf in response to salt stress,all members of WRKY family were identified and their expression patterns were analyzed.Physical and chemical properties,phylogeny and conserved functional domains of WRKY gene family members were analyzed by bioinformatics method.The expression characteristics of WRKY gene family members under salt stress were analyzed by RT-PCR.The results showed that a total of 33 WRKY family members were identified,which were unevenly distributed on 12 chromosomes.There were certain differences in the physical and chemical properties of each member,such as amino acid number,molecular weight and theoretical isoelectric point.The conserved sequence WRKYGQK of each member did not change.Phylogenetic tree analysis showed that 33 WRKY family members were divided into 3 groups,GroupⅠ,GroupⅡ,Group Ⅲ,of which Group Ⅲ contained 5 subgroups.The real-time fluorescence quantitative RT-PCR results showed that there were 26 WRKY family members induced by salt stress,of which 23 had positive regulation and 3 had negative regulation.A total of 33 WRKY family members of kenaf were identified,of which 26 WRKY family members were involved in the salt stress response of kenaf.

Key words: Kenaf, WRKY, Bioinformatics, Salt stress