[1] Eulgem T,Somssich I E.Networks of WRKY transcription factors in defense signaling[J]. Current Opinion in Plant Biology,2007,10(4):366-371.doi:10.1016/j.pbi.2007.04.020. [2] Ross C A,Liu Y,Shen Q J.The WRKY gene family in rice(Oryza sativa)[J]. Journal of Integrative Plant Biology,2007,49(6):827-842.doi:10.1111/j.1744-7909.2007.00504.x. [3] Bencke-Malato M,Cabreira C,Wiebke-Strohm B,Bücker-Neto L,Mancini E,Osorio M B,Homrich M S,Turchetto-Zolet A C,De Carvalho M C C G,Stolf R,Weber R L M,Westergaard G,Castagnaro A P,Abdelnoor R V,Marcelino-Guimar a es F C,Margis-Pinheiro M,Bodanese-Zanettini M H.Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection[J]. BMC Plant Biology,2014,14:236.doi:10.1186/s12870-014-0236-0. [4] Wei K F,Chen J,Chen Y F,Wu L J,Xie D X.Molecular phylogenetic and expression analysis of the complete WRKY transcription factor family in maize[J]. DNA Research,2012,19(2):153-164.doi:10.1093/dnares/dsr048. [5] Ning P,Liu C C,Kang J Q,Lü J Y.Genome-wide analysis of WRKY transcription factors in wheat(Triticum aestivum L.) and differential expression under water deficit condition[J]. Peer J,2017,5:e3232.doi:10.7717/peerj.3232. [6] Chen N,Su M W,Chi X Y,Zhang Z M,Pan L J,Chen M N,Wang T,Wang M,Yang Z,Yu S L.Transcriptome analysis reveals salt-stress-regulated biological processes and key pathways in roots of peanut (Arachis hypogaea L.)[J]. Genes & Genomics,2016,38(6):493-507.doi:10.1007/s13258-016-0395-5. [7] Lagacé M,Matton D P.Characterization of a WRKY transcription factor expressed in late Torpedo-stage embryos of Solanum chacoense[J]. Planta,2004,219(1):185-189.doi:10.1007/s00425-004-1253-2. [8] Zentella R,Zhang Z L,Park M,Thomas S G,Endo A,Murase K,Fleet C M,Jikumaru Y,Nambara E,Kamiya Y,Sun T P.Global analysis of DELLA direct targets in early gibberellin signaling in Arabidopsis[J]. The Plant Cell,2007,19(10):3037-3057.doi:10.1105/tpc.107.054999. [9] Johnson C S,Kolevski B,Smyth D R.TRANSPARENT TESTA GLABRA2,a trichome and seed coat development gene of Arabidopsis,encodes a WRKY transcription factor[J]. The Plant Cell,2002,14(6):1359-1375.doi:10.1105/tpc.001404. [10] Robatzek S,Somssich I E.Targets of AtWRKY6 regulation during plant senescence and pathogen defense[J]. Genes & Development,2002,16(9):1139-1149.doi:10.1101/gad.222702. [11] Lai Z B,Li Y,Wang F,Cheng Y,Fan B F,Yu J Q,Chen Z X. Arabidopsis Sigma factor binding proteins are activators of the WRKY33 transcription factor in plant defense[J]. The Plant Cell,2011,23(10):3824-3841.doi:10.1105/tpc.111.090571. [12] Rushton D L,Tripathi P,Rabara R C,Lin J,Ringler P,Boken A K,Langum T J,Smidt L,Boomsma D D,Emme N J,Chen X F,Finer J J,Shen Q J,Rushton P J.WRKY transcription factors:Key components in abscisic acid signalling[J]. Plant Biotechnology Journal,2012,10(1):2-11.doi:10.1111/j.1467-7652.2011.00634.x. [13] Rinerson C I,Rabara R C,Tripathi P,Shen Q J,Rushton P J.The evolution of WRKY transcription factors[J]. BMC Plant Biology,2015,15:66.doi:10.1186/s12870-015-0456-y. [14] Kloth K J,Wiegers G L,Busscher-Lange J,van Haarst J C,Kruijer W,Bouwmeester H J,Dicke M,Jongsma M A.AtWRKY22 promotes susceptibility to aphids and modulates salicylic acid and jasmonic acid signalling[J]. Journal of Experimental Botany,2016,67(11):3383-3396.doi:10.1093/jxb/erw159. [15] Pandey S P,Somssich I E.The role of WRKY transcription factors in plant immunity[J]. Plant Physiology,2009,150(4):1648-1655.doi:10.1104/pp.109.138990. [16] Birkenbihl R P,Diezel C,Somssich I E. Arabidopsis WRKY33 is a key transcriptional regulator of hormonal and metabolic responses toward Botrytis cinerea infection[J]. Plant Physiology,2012,159(1):266-285.doi:10.1104/pp.111.192641. [17] Rushton P J,Torres J T,Parniske M,Wernert P,Hahlbrock K,Somssich I E.Interaction of elicitor-induced DNA-binding proteins with elicitor response elements in the promoters of parsley PR1 genes[J]. EMBO J,1996,15(20):5690-5700.doi:10.1002/j.1460-2075.1996.tb00953.x. [18] Chen L G,Song Y,Li S J,Zhang L P,Zou C S,Yu D Q.The role of WRKY transcription factors in plant abiotic stresses[J]. Biochimica et Biophysica Acta,2012,1819(2):120-128.doi:10.1016/j.bbagrm.2011.09.002. [19] 刘晓林,杨学勇,余宏军,蒋卫杰,赵文超,王燕,张晓孟.适用于黄瓜叶片、根系和果实总蛋白的双向电泳体系的建立[J].基因组学与应用生物学,2016,35(3):721-727.doi:10.13417/j.gab.035.000721. Liu X L,Yang X Y,Yu H J,Jiang W J,Zhao W C,Wang Y,Zhang X M.Establishment of two-dimensional electrophoresis system suitable for cucumber leave,root and fruit proteins[J]. Genomics and Applied Biology,2016,35(3):721-727. [20] Jiang Y Q,Deyholos M K.Functional characterization of Arabidopsis NaCl-inducible WRKY25 and WRKY33 transcription factors in abiotic stresses[J]. Plant Molecular Biology,2009,69(1/2):91-105.doi:10.1007/s11103-008-9408-3. [21] Luo X,Bai X,Sun X L,Zhu D,Liu B H,Ji W,Cai H,Cao L,Wu J,Hu M R,Liu X,Tang L L,Zhu Y M.Expression of wild soybean WRKY20 in Arabidopsis enhances drought tolerance and regulates ABA signalling[J]. Journal of Experimental Botany,2013,64(8):2155-2169.doi:10.1093/jxb/ert073. [22] Qin Y X,Tian Y C,Han L,Yang X C.Constitutive expression of a salinity-induced wheat WRKY transcription factor enhances salinity and ionic stress tolerance in transgenic Arabidopsis thaliana[J]. Biochemical and Biophysical Research Communications,2013,441(2):476-481. [23] Zhou Q Y,Tian A G,Zou H F,Xie Z M,Lei G,Huang J,Wang C M,Wang H W,Zhang J S,Chen S Y.Soybean WRKY-type transcription factor genes, GmWRKY13,GmWRKY21, and GmWRKY54, confer differential tolerance to abiotic stresses in transgenic Arabidopsis plants[J]. Plant Biotechnology Journal,2008,6(5):486-503.doi:10.1111/j.1467-7652.2008.00336.x. [24] Bolton J L.Alfalfa botany,cultivation and utilization[M].London:Leonard Hill,1962. [25] 于浩然,贾玉山,贾鹏飞,连直,卢强,李俊峰,王志军,任志花.不同盐碱度对紫花苜蓿产量及品质的影响[J].中国草地学报,2019,41(4):143-149.doi:10.16742/j.zgcdxb.20180240. Yu H R,Jia Y S,Jia P F,Lian Z,Lu Q,Li J F,Wang Z J,Ren Z H.Comprehensive evaluation of growth,yield and quality of alfalfa in different saline-alkali soil[J]. Chinese Journal of Grassland,2019,41(4):143-149. [26] Wang X S,Ren H L,Wei Z W,Wang Y W,Ren W B.Effects of neutral salt and alkali on ion distributions in the roots,shoots,and leaves of two alfalfa cultivars with differing degrees of salt tolerance[J]. Journal of Integrative Agriculture,2017,16(8):1800-1807.doi:10.1016/S2095-3119(16) 61522-8. [27] 董蔚,邬培祥,杨宁,刘锡江,宋玉光.紫花苜蓿盐胁迫响应WRKY转录因子的克隆及表达特征分析[J].植物生理学报,2018,54(9):1481-1489.doi:10.13592/j.cnki.ppj.2018.0306. Dong W,Wu P X,Yang N,Liu X J,Song Y G.Cloning and expression analysis of WRKY transcription factor involved in salinity stress in alfalfa[J]. Plant Physiology Journal,2018,54(9):1481-1489. [28] 程彦伟,李亮,沈嵘,齐耀程,刘晓宇,王宁,张炜.水稻LRR型类受体蛋白激酶胞外区的原核表达及多克隆抗体制备[J].生物化学与生物物理进展,2008,35(9):1077-1083.doi:10.3321/j.issn:1000-3282.2008.09.016. Cheng Y W,Li L,Shen R,Qi Y C,Liu X Y,Wang N,Zhang W.Prokaryotic expression and polyclonal antibody preparation of the extracellular domain about rice LRR receptor-like protein kinase[J]. Progress in Biochemistry and Biophysics,2008,35(9):1077-1083. [29] 王增,代茹,张江巍,陈尚武,张文,马会勤.拟南芥WUS蛋白的原核表达、亲和纯化和多克隆抗体制备[J].生物工程学报,2009,25(9):1409-1416.doi:10.3321/j.issn:1000-3061.2009.09.019. Wang Z,Dai R,Zhang J W,Chen S W,Zhang W,Ma H Q.Induced expression of Arabidopsis thaliana WUSCHEL in Escherichia coli,affinity protein purification and polyclonal antibody preparation[J]. Chinese Journal of Biotechnology,2009,25(9):1409-1416. [30] Burnette W N.Western Blotting:Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A[J]. Analytical Biochemistry,1981,112(2):195-203.doi:10.1016/0003-2697(81) 90281-5. [31] Bronstein I,Voyta J C,Murphy O J,Bresnick L,Kricka L J.Improved chemiluminescent western blotting procedure[J]. Biotechniques,1992,12(5):748-753. [32] 冯光燕,王学敏,付媛媛,方志红,高洪文,张新全.紫花苜蓿MsWRKY33转录因子的分离及遗传转化研究[J].草业学报,2015,24(11):48-57.doi:10.11686/cyxb2015131. Feng G Y,Wang X M,Fu Y Y,Fang Z H,Gao H W,Zhang X Q.Isolation of MsWRKY33 transcription factor and its genetic transformation in Medicago sativa[J]. Acta Prataculturae Sinica,2015,24(11):48-57. [33] Chen F,Hu Y,Vannozzi A,Wu K C,Cai H Y,Qin Y,Mullis A,Lin Z G,Zhang L S.The WRKY transcription factor family in model plants and crops[J]. Critical Reviews in Plant Sciences,2017,36(5/6):311-335.doi:10.1080/07352689.2018.1441103. [34] Zhu J K.Salt and drought stress signal transduction in plants[J]. Annual Review of Plant Biology,2002,53:247-273.doi:10.1146/annurev.arplant.53.091401.143329. |