油用向日葵脱落酸代谢及差异基因表达对水分亏缺的响应

doi:10.7668/hbnxb.20192019

摘要/Abstract

摘要： 为了解油用向日葵应对干旱胁迫的脱落酸代谢差异基因挖掘及分子调控机理，对正常供水（CK）和干旱胁迫（T）处理下的油用向日葵进行了高通量转录组测序及生理特性测定。结果表明，脱落酸（ABA）含量在干旱胁迫8 d最高，为23.55 ng/mL，在受到干旱胁迫4，8 d时的脱落酸含量均高于0 d。对差异表达基因进行筛选，在干旱胁迫8，16 d，通过与CK比较，处理组较CK显著上调表达的差异基因数分别为940，1 743个，显著下调表达的基因数分别为1 752，3 037个。由KEGG、GO功能注释可知，在干旱胁迫16 d较8 d的基因种类更丰富，其中参与生物过程的最多，参与细胞组分的次之，参与分子功能的最少，KEGG注释到的主要代谢通路有植物MAPK信号传导途径、植物激素信号转导、甘油磷脂代谢等。对干旱胁迫8 d条件下与激素相关的基因进行分析，共找到差异表达基因39个，并有7个基因与脱落酸代谢相关；参与脱落酸代谢的转录因子为ABF2、SAPK2、PP2C、AHG1、PYL2、SAPK3均呈上调表达，并表现出受到干旱胁迫的表达量高于CK。本研究挖掘到油用向日葵干旱胁迫下的ABA代谢相关上调差异基因7个，脱落酸代谢过程（PYR/PYL→PP2C→SnRK2→ABF）中涉及的转录因子均表现为上调，说明植物在遭受干旱胁迫时，会通过脱落酸等激素的积累以抵御干旱胁迫。

关键词: 油用向日葵, 干旱胁迫, 转录组测序, 激素代谢, 脱落酸

Abstract: In order to understand the differential gene mining and molecular regulation mechanism of abscisic acid metabolism of oil sunflower in response to drought stress, high-throughput transcriptome sequencing and physiological characteristics were measured on oil sunflower under normal water supply (CK) and drought stress (T) treatments. The results showed that the content of abscisic acid (ABA) was the highest at 8 d of drought stress, at 23.55 ng/mL, and higher than 0 d at 4,8 d of drought stress. The differentially expressed genes were screened and compared with CK on 8 and 16 days of drought stress, the number of differentially expressed genes in the treatment group was 940, 1 743, and the number of genes that were significantly down-regulated was 1 752, 3 037, respectively. According to the functional annotations of KEGG and GO, there were more gene types under drought stress at 16 d than at 8 d. Among them, the most involved biological processes, the second was involved in cell components, and the least involved in molecular functions. The main metabolic pathways annotated by KEGG were plant MAPK signal transduction pathway, plant hormone signal transduction, glycerophospholipid metabolism and so on. Analyzed the hormone-related genes under drought stress for 8 days. A total of 39 differentially expressed genes were found, and 7 genes were related to abscisic acid metabolism. The transcription factors involved in abscisic acid metabolism were ABF2, SAPK2, PP2C, AHG1, PYL2 and SAPK3 were up-regulated, and the expression level of drought stress was higher than that of CK. The conclusion of this study was that 7 different genes related to the up-regulation of ABA metabolism in oil sunflower under drought stress were discovered. The transcription factors involved in the metabolism of abscisic acid (PYR/PYL→PP2C→SnRK2→ABF) were all up-regulated, indicating that the plant when suffering from drought stress, it would resist drought stress by accumulating hormones such as abscisic acid.

Key words: Oil sunflower, Drought stress, Transcriptome sequencing, Hormone metabolism, Abscisic acid

中图分类号:

Q78
S565.03

赵雅杰, 赵轩微, 田振东, 胡树平, 包海柱. 油用向日葵脱落酸代谢及差异基因表达对水分亏缺的响应[J]. 华北农学报, 2021, 36(6): 54-62. doi: 10.7668/hbnxb.20192019.

ZHAO Yajie, ZHAO Xuanwei, TIAN Zhendong, HU Shuping, BAO Haizhu. Abscisic Acid Metabolism and Differential Gene Expression Response to Water Deficit in the Oil Sunflower[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(6): 54-62. doi: 10.7668/hbnxb.20192019.

http://www.hbnxb.net/CN/Y2021/V36/I6/54

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