异源表达苎麻金属硫蛋白<i>BnMLP2</i>基因增强拟南芥对干旱胁迫的耐受性

doi:10.7668/hbnxb.20195314

摘要/Abstract

摘要：

为探究BnMLP2基因在苎麻抗旱过程中的作用,通过分析苎麻转录组数据,以中苎1号为材料得到了编码苎麻金属硫蛋白的BnMLP2基因。从苎麻转录组数据中筛选并克隆BnMLP2基因,进行生物信息学分析,包括序列比对、结构域预测及亚细胞定位预测。利用荧光定量PCR技术,测定BnMLP2基因在苎麻不同组织中的表达情况,并探究其在干旱胁迫下的表达变化。将BnMLP2基因导入拟南芥中,构建转基因植株。在干旱胁迫条件下,比较转基因拟南芥与野生型拟南芥的表型、生理生化指标差异,以及抗逆相关基因的表达情况。结果表明,苎麻BnMLP2基因开放阅读框全长共243 bp,编码80个氨基酸。BnMLP2与苹果MT或MLP的氨基酸序列亲缘关系最近,同属于金属硫蛋白家族成员;带有PFAM01439结构域,属于Ⅱ类金属硫蛋白;预测定位于细胞质。BnMLP2基因在苎麻各组织中均表达,并且BnMLP2的表达受干旱显著诱导,尤其在茎中的表达量最高。在干旱胁迫下,转BnMLP2拟南芥相较于野生型表现出更强的抗旱能力,具体表现为根长和鲜质量显著增加,表现出更强的抗氧化酶和γ-GCL活性,积累了更多的脯氨酸(Pro)、还原型谷胱甘肽(GSH)、氧化型谷胱甘肽(GSSG)、植物螯合态(PCs)来调节细胞内的离子稳态,转基因株系丙二醛(MDA)、过氧化氢(H₂O₂)的含量显著低于野生型,约为野生型的55%,80%。此外,转基因拟南芥在干旱条件下钠离子和钾离子的含量最高为野生型的4.4,1.4倍。BnMLP2的过表达能诱导3个抗逆相关基因AtMT1a、AtNCED3、AtWRKY40的表达量提高,最高分别是野生型的1.5,1.9,2.8倍。上述结果表明,BnMLP2基因能够提高拟南芥对干旱胁迫的耐受能力。

关键词: 苎麻, 金属硫蛋白, 干旱胁迫, 耐受性, 异源表达

Abstract:

To investigate the role of the BnMLP2 gene in drought tolerance in ramie,the BnMLP2 gene encoding a metallothionein-like protein in ramie was obtained by analyzing ramie transcriptome data using Zhongzhu-1 as the material.The BnMLP2 gene was screened and cloned from the ramie transcriptome data,and bioinformatics analyses were conducted,including sequence alignment,domain prediction,and subcellular localization prediction.Fluorescent Quantitative PCR was used to determine the expression profile of the BnMLP2 gene in different tissues of ramie and to explore its expression changes under drought stress.The BnMLP2 gene was introduced into Arabidopsis thaliana to construct transgenic plants.Under drought stress conditions,phenotypic,physiological,and biochemical differences between transgenic and wild-type Arabidopsis were compared,along with the expression of stress-related genes.The results showed that the open reading frame of the BnMLP2 gene in ramie was 243 bp in length,encoding 80 amino acids.BnMLP2 had the closest amino acid sequence homology to metallothionein(MT)or metallothionein-like protein (MLP) from Malus domestica,both belonging to the metallothionein family; it contained the PFAM01439 domain and belonged to class Ⅱ metallothionein,with a predicted subcellular localization in the cytoplasm.The BnMLP2 gene was expressed in all tissues of ramie,and its expression was significantly induced by drought,especially in stems.Under drought stress,transgenic Arabidopsis overexpressing BnMLP2 exhibited stronger drought tolerance compared to wild-type plants,as evidenced by significantly increased root length and fresh weight,enhanced antioxidant enzyme and γ-glutamylcysteine ligase (γ-GCL) activities,and accumulation of more proline (Pro),glutathione(GSH),glutathione disulfide (GSSG),and phytochelatins (PCs) to regulate intracellular ion homeostasis.The contents of malondialdehyde (MDA)and hydrogen peroxide (H₂O₂)in transgenic lines were significantly lower than in wild-type plants,at 55% and 80% of wild-type levels,respectively.In addition, the content of sodium and potassium ions in transgenic Arabidopsis under drought conditions was 4.4 times and 1.4 times higher than that of the wild type, respectively. Overexpression of BnMLP2 induced increased expression of three stress-related genes,AtMT1a,AtNCED3,and AtWRKY40,with maximum expression levels of 1.5,1.9,and 2.8 times those in wild-type plants,respectively.These results suggest that the BnMLP2 gene enhances the tolerance of Arabidopsis to drought stress.

Key words: Boehmeria nivea, Metallothionein, Drought stress, Tolerance, Heterologous expression

中图分类号:

王立琦, 张蕾, 李茜, 管圣, 卢建宁, 许明志, 崔国贤, 佘玮. 异源表达苎麻金属硫蛋白BnMLP2基因增强拟南芥对干旱胁迫的耐受性[J]. 华北农学报, 2025, 40(3): 19-26. doi: 10.7668/hbnxb.20195314.

WANG Liqi, ZHANG Lei, LI Xi, GUAN Sheng, LU Jianning, XU Mingzhi, CUI Guoxian, SHE Wei. Heterologous Expression of Ramie Metallothionein BnMLP2 Gene Enhances Arabidopsis Tolerance to Drought Stress[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(3): 19-26. doi: 10.7668/hbnxb.20195314.

http://www.hbnxb.net/CN/Y2025/V40/I3/19

图/表 8

表1 本研究中所用到的引物

Tab.1 Primers used in this study

引物名称 Primer name	引物序列(5'—3') Primer sequence(5'—3')	引物用途 Usage or purpose
HTP-F(855)	GGAGGGCGAAGAATCTCGTG	转基因拟南芥筛选
HTP-R(855)	ACTTCTACACAGCCATCGGT
AtMT1a-F	TCCTGCAAATGTGGTGACTCT	拟南芥qPCR
AtMT1a-R	ACCCACAGCTGCAGTTTGAT
AtMT2a-F	AATTTGGGAGCTTCCTCTATCATT
AtMT2a-R	CAGGGTACATTTTGCAACCTCC
AtMT3a-F	AATTTGGGAGCTTCCTCTATCATT
AtMT3a-R	CAGGGTACATTTTGCAACCTCC
AtNCED3-F	TGTCCTGTCTGAAATCCGCC
AtNCED3-R	TTACGGCCGAGCATGTTTCT
AtWRKY40-F	TGGCTTAAACCGCCACATCT
AtWRKY40-R	ATTCTTGACGTTGGGCTCGT
ACTIN8-F	TCAGCACTTTCCAGCAGATG	拟南芥内参基因
ACTIN8-R	CTGTGGACAATGCCTGGAC
BnMLP2-F	ACTCTCATCATCGGTGTCGC	苎麻qPCR
BnMLP2-R	GGGTCGCAGGAACAGCTATC
Actin-F	GCTCCGTTGAACCCTAAG	苎麻内参基因
Actin-R	GCTCCGATTGTGATGATTT
35S-BnMLP2-EcoR ⅠF	CAAGAGACAGGATCCGAATTCGAAAATGTCTTGCTGCGGAGG	BnMLP2克隆
35S-BnMLP2-Sal ⅠR	CATCGGTGCACTAGTGTCGACTTTACAAGTGCAGGGGTCGCA

图1 苎麻BnMLP2氨基酸序列与其他植物MT氨基酸序列同源性比较黑色.序列相似性为100%;粉红色.序列相似性大于75%;蓝色.序列相似性大于50%。

Fig.1 Comparison of homology between BnMLP2 amino acid sequences in ramie and MT amino acid sequences in other plants Black.100% sequence homology;Pink.Sequence homology greater than 75%;Blue.Sequence homology greater than 50%.

图2 BnMLP2与其他植物MLP蛋白的系统进化树

Fig.2 Phylogenetic tree of BnMLP2 and other plant MLP proteins

图3 干旱胁迫下苎麻不同器官BnMLP2的表达情况相同处理内不同小写字母表示在P<0.05水平差异显著。图4—5,7同。

Fig.3 Expression of BnMLP2 in different organs of ramie under drought stress Different lowercase letters within the same treatments indicate significant differences at the 0.05 level.The same as Fig.4—5,7.

图4 干旱胁迫下野生型与过表达BnMLP2转基因拟南芥的表型与形态指标

Fig.4 Phenotypic and morphological indicators of wild-type and BnMLP2 transgenic Arabidopsis under drought stress

图5 干旱胁迫下过表达BnMLP2拟南芥的抗氧化及胁迫相关生理指标

Fig.5 Antioxidant and stress-related physiological indicators of overexpressing BnMLP2 in Arabidopsis thaliana under drought stress

图6 干旱胁迫下过表达BnMLP2拟南芥钠和钾含量不同小写字母表示不同基因型之间差异显著(P<0.05)。

Fig.6 Contents of sodium and potassium in Arabidopsis with overexpression of BnMLP2 gene under drought stress Different lowercase letters indicate significant differences between different genotypes(P<0.05).

图7 干旱胁迫下过表达BnMLP2拟南芥干旱响应相关基因表达量

Fig.7 Expression levels of genes related to stress response in Arabidopsis with overexpression of BnMLP2 gene under drought stress

参考文献 31

[1]	佘玮, 崔国贤, 赵丹博, 肖呈祥. 锌、铁缺失对苎麻吸收及转运重金属镉的影响[J]. 农业环境科学学报, 2014, 33(2):283-287.doi:10.11654/jaes.2014.02.012.
	She W, Cui G X, Zhao D B, Xiao C X. Cadmium uptake and transportation in two ramie cultivars under zinc and iron deficiency[J]. Journal of Agro-Environment Science, 2014, 33(2):283-287.
[2]	谢傲, 罗伯良, 邓剑波, 高霞霞. 湖南2022/2023年夏秋冬季持续极端干旱事件特征及成因分析[J]. 干旱气象, 2023, 41(6):910-922.doi:10.11755/j.issn.1006-7639(2023)-06-0910.
	Xie A, Luo B L, Deng J B, Gao X X. Characteristics and cause analysis of extreme and persistent drought in summer,autumn and winter in 2022/2023 in Hunan Province[J]. Journal of Arid Meteorology, 2023, 41(6):910-922.
[3]	Cobbett C, Goldsbrough P. Phytochelatins and metallothioneins:roles in heavy metal detoxification and homeostasis[J]. Annual Review of Plant Biology, 2002, 53:159-182.doi:10.1146/annurev.arplant.53.100301.135154.
[4]	Freisinger E. Structural features specific to plant metallothioneins[J]. Journal of Biological Inorganic Chemistry, 2011, 16(7):1035-1045.doi:10.1007/s00775-011-0801-z. pmid: 21688177
[5]	Casterline J L, Barnett N M. Cadmium-binding components in soybean plants[J]. Plant Physiology, 1982, 69(5):1004-1007.doi:10.1104/pp.69.5.1004. pmid: 16662333
[6]	Zimeri A M, Dhankher O P, McCaig B, Meagher R B. The plant MT1 metallothioneins are stabilized by binding cadmiums and are required for cadmium tolerance and accumulation[J]. Plant Molecular Biology, 2005, 58(6):839-855.doi:10.1007/s11103-005-8268-3. pmid: 16240177
[7]	Cheng M X, Yuan H R, Wang R H, Zou J N, Liang T, Yang F, Li S Q. Genome-wide identification and analysis of the metallothionein genes in Oryza genus[J]. International Journal of Molecular Sciences, 2021, 22(17):9651.doi:10.3390/ijms22179651.
[8]	Li R, Yang Y, Cao H P, Peng X, Yu Q, He L S, Chen J, Xiang L E, Liu W H. Heterologous expression of the tobacco metallothionein gene NtMT2F confers enhanced tolerance to Cd stress in Escherichia coli and Arabidopsis thaliana[J]. Plant Physiology and Biochemistry, 2023, 195:247-255.doi:10.1016/j.plaphy.2023.01.027.
[9]	Ruttkay-Nedecky B, Nejdl L, Gumulec J, Zitka O, Masarik M, Eckschlager T, Stiborova M, Adam V, Kizek R. The role of metallothionein in oxidative stress[J]. International Journal of Molecular Sciences, 2013, 14(3):6044-6066.doi:10.3390/ijms14036044. pmid: 23502468
[10]	Li Y, Chen Y Y, Yang S G, Tian W M. Cloning and characterization of HbMT2a,a metallothionein gene from Hevea brasiliensis Muell.Arg differently responds to abiotic stress and heavy metals[J]. Biochemical and Biophysical Research Communications, 2015, 461(1):95-101.doi:10.1016/j.bbrc.2015.03.175. pmid: 25858315
[11]	Kumar S, Yadav A, Verma R, Dubey A K, Narayan S, Pandey A, Sahu A, Srivastava S, Sanyal I. Metallothionein (MT1):a molecular stress marker in chickpea enhances drought and heavy metal stress adaptive efficacy in transgenic plants[J]. Environmental and Experimental Botany, 2022, 199:104871.doi:10.1016/j.envexpbot.2022.104871.
[12]	Yang X Y, Lu M Q, Wang Y F, Wang Y R, Liu Z J, Chen S. Response mechanism of plants to drought stress[J]. Horticulturae, 2021, 7(3):50.doi:10.3390/horticulturae7030050.
[13]	Liu J, Shi X T, Qian M, Zheng L Q, Lian C L, Xia Y, Shen Z G. Copper-induced hydrogen peroxide upregulation of a metallothionein gene,OsMT2c,from Oryza sativa L.confers copper tolerance in Arabidopsis thaliana[J]. Journal of Hazardous Materials, 2015, 294:99-108.doi:10.1016/j.jhazmat.2015.03.060.
[14]	Peng J S, Ding G, Meng S, Yi H Y, Gong J M. Enhanced metal tolerance correlates with heterotypic variation in SpMTL,a metallothionein-like protein from the hyperaccumulator Sedum plumbizincicola[J]. Plant,Cell & Environment, 2017, 40(8):1368-1378.doi:10.1111/pce.12929.
[15]	Jaiswal P S, Mittal N, Randhawa G S. Cyamopsis tetragonoloba type 1 metallothionein (CtMT1) gene is upregulated under drought stress and its protein product has an additional C-X-C motif and unique metal binding pattern[J]. International Journal of Biological Macromolecules, 2018, 119:1324-1334.doi:10.1016/j.ijbiomac.2018.08.027.
[16]	Mekawy A M M, Assaha D V M, Ueda A. Constitutive overexpression of rice metallothionein-like gene OsMT-3a enhances growth and tolerance of Arabidopsis plants to a combination of various abiotic stresses[J]. Journal of Plant Research, 2020, 133(3):429-440.doi:10.1007/s10265-020-01187-y.
[17]	唐中华, 郭晓瑞, 张洋洋, 安志刚, 于景华, 祖元刚. 拟南芥MT-Ⅱ过量表达提高抗旱性(英文)[J]. 植物研究, 2005, 25(4):415-418.doi:10.7525/j.issn.1673-5102.2005.04.008.
	Tang Z H, Guo X R, Zhang Y Y, An Z G, Yu J H, Zu Y G. Expression of metallothionein-Ⅱ in Arabidopsis thaliana improve desiccation tolerance[J]. Bulletin of Botanical Research, 2005, 25(4):415-418.
[18]	Hassinen V H, Tervahauta A I, Schat H, Kärenlampi S O. Plant metallothioneins-metal chelators with ROS scavenging activity?[J]. Plant Biology, 2011, 13(2):225-232.doi:10.1111/j.1438-8677.2010.00398.x.
[19]	刘阳, 彭翠, 吴彦辰, 邓夕莞, 毛新芳, 刘忠渊. 盐穗木金属硫蛋白HcMT的体外自由基清除活性及抗氧化能力[J]. 中国生物工程杂志, 2022, 42(9):17-26.doi:10.13523/j.cb.2204041.
	Liu Y, Peng C, Wu Y C, Deng X W, Mao X F, Liu Z Y. Free radical scavenging activity and antioxidant capacity of metallothionein HcMT from Halostachys caspica in vitro[J]. China Biotechnology, 2022, 42(9):17-26.
[20]	Dubey A K, Kumar N, Kumar A, Ansari M A, Ranjan R, Gautam A, Meenakshi, Sahu N, Pandey V, Behera S K, Mallick S, Pande V, Sanyal I. Over-expression of CarMT gene modulates the physiological performance and antioxidant defense system to provide tolerance against drought stress in Arabidopsis thaliana L.[J]. Ecotoxicology and Environmental Safety, 2019, 171:54-65.doi:10.1016/j.ecoenv.2018.12.050.
[21]	Qin S Y, Liu H E, Nie Z J, Gao W, Li C, Lin Y H, Zhao P. AsA-GSH cycle and antioxidant enzymes play important roles in Cd tolerance of wheat[J]. Bulletin of Environmental Contamination and Toxicology, 2018, 101(5):684-690.doi:10.1007/s00128-018-2471-9. pmid: 30353306
[22]	Jiang Z Z, Zhu H G, Zhu H Y, Tao Y Z, Liu C Z, Liu J Q, Yang F Q, Li M. Exogenous ABA enhances the antioxidant defense system of maize by regulating the AsA-GSH cycle under drought stress[J]. Sustainability, 2022, 14(5):3071.doi:10.3390/su14053071.
[23]	Guo W J, Bundithya W, Goldsbrough P B. Characterization of the Arabidopsis metallothionein gene family:tissue-specific expression and induction during senescence and in response to copper[J]. New Phytologist, 2003, 159(2):369-381.doi:10.1046/j.1469-8137.2003.00813.x.
[24]	Kim Y O, Kang H. Comparative expression analysis of genes encoding metallothioneins in response to heavy metals and abiotic stresses in rice (Oryza sativa) and Arabidopsis thaliana[J]. Bioscience,Biotechnology,and Biochemistry, 2018, 82(9):1656-1665.doi:10.1080/09168451.2018.1486177.
[25]	赵良侠, 琚唐丹, 熊蕾, 高坤, 贾兆朴, 凌峰, 马国庆, 郭宏伟, 高也, 高灿红. 非生物胁迫下植物金属硫蛋白基因调控的研究进展[J]. 植物生理学报, 2022, 58(10):1801-1817.doi:10.13592/j.cnki.ppj.300081.
	Zhao L X, Ju T D, Xiong L, Gao K, Jia Z P, Ling F, Ma G Q, Guo H W, Gao Y, Gao C H. Research advances on gene regulation of metallothionein in plants under abiotic stress[J]. Plant Physiology Journal, 2022, 58(10):1801-1817.
[26]	Liu J Y, Zhang J, Kim S H, Lee H S, Marinoia E, Song W Y. Characterization of Brassica rapa metallothionein and phytochelatin synthase genes potentially involved in heavy metal detoxification[J]. PLoS One, 2021, 16(6):e0252899.doi:10.1371/journal.pone.0252899.
[27]	Lee S U, Mun B G, Bae E K, Kim J Y, Kim H H, Shahid M, Choi Y I, Hussain A, Yun B W. Drought stress-mediated transcriptome profile reveals NCED as a key player modulating drought tolerance in Populus davidiana[J]. Frontiers in Plant Science, 2021, 12:755539.doi:10.3389/fpls.2021.755539.
[28]	Wani S H, Anand S, Singh B, Bohra A, Joshi R. WRKY transcription factors and plant defense responses:latest discoveries and future prospects[J]. Plant Cell Reports, 2021, 40(7):1071-1085.doi:10.1007/s00299-021-02691-8.
[29]	赵爽, 葛朝红, 石鹤飞, 闵卓, 王广鹏, 李伟明. 板栗WRKY基因家族鉴定及其在干旱胁迫下的表达分[J]. 华北农学报, 2024, 39(1): 72-82. doi: 10.7668/hbnxb.20194000.
	Zhao S, Ge C H, Shi H F, Min Z, Wang G P, Li W M. Identification and expression analysis of the chestnut WRKY gene family under drought stress[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(1): 72-82. doi: 10.7668/hbnxb.20194000
[30]	车永梅, 孙艳君, 卢松冲, 赵方贵, 侯丽霞, 刘新. AtWRKY40参与拟南芥干旱胁迫响应过程[J]. 植物生理学报, 2018, 54(3):456-464.doi:10.13592/j.cnki.ppj.2017.0360.
	Che Y M, Sun Y J, Lu S C, Zhao F G, Hou L X, Liu X. AtWRKY40 functions in drought stress response in Arabidopsis thaliana[J]. Plant Physiology Journal, 2018, 54(3):456-464.
[31]	Mukherjee A, Dwivedi S, Bhagavatula L, Datta S. Integration of light and ABA signaling pathways to combat drought stress in plants[J]. Plant Cell Reports, 2023, 42(5):829-841.doi:10.1007/s00299-023-02999-7.

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异源表达苎麻金属硫蛋白BnMLP2基因增强拟南芥对干旱胁迫的耐受性

Heterologous Expression of Ramie Metallothionein BnMLP2 Gene Enhances Arabidopsis Tolerance to Drought Stress

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异源表达苎麻金属硫蛋白BnMLP2基因增强拟南芥对干旱胁迫的耐受性

Heterologous Expression of Ramie Metallothionein BnMLP2 Gene Enhances Arabidopsis Tolerance to Drought Stress

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