摘要： 为探讨转脂蛋白在非生物逆境中的作用，将ZmLTP3基因利用花粉管通道法转入玉米自交系京2416中，在PCR和EPSPS速测试纸鉴定基础上连续自交获得转基因阳性株系。以转基因株系OE6、OE10、OE18作为试验组，以自交系京2416为对照（WT），进行盐胁迫处理，比较二者之间在形态指标和生理指标方面的差异。结果显示，在正常生长条件下，转基因株系与野生型的各项指标均无显著差异。在盐胁迫条件下，与野生型相比，转基因株系的株高、茎基宽、根长、鲜质量、干质量及叶绿素含量均显著增加，表现出较好的生长状态；超氧化物歧化酶（SOD）、过氧化物酶（POD）及过氧化氢酶（CAT）活性也显著升高，显示出较高的活性氧清除能力；同时，丙二醛（MDA）含量及相对外渗电导率水平则显著下降，暗示其体内较低程度的细胞伤害水平。以上结果表明，ZmLTP3基因的过量表达可提高玉米的耐盐胁迫能力。

关键词: ZmLTP3基因, 花粉管通道法, 转基因玉米, 耐盐性

Abstract: To study the function of lipid transfer proteins(LTP)in abiotic stress,the ZmLTP3 gene was transferred into maize Jing 2416 inbred lines by pollen tube pathway method. Positive transgenic lines were obtained by continuous selfing based on the screening of PCR and EPSPS. Three transgenic lines OE6,OE10 and OE18 were selected as the experimental group,and Jing 2416 inbred lines(WT)were used as CK. Both of them were subjected to salt stress. Morphological and physiological indexes were compared between OE and WT lines. The results showed that OE and WT plants showed no significant differences in both morphological and physiological indexes under normal condition. Under the salt stress condition,compared with WT plants,the OE plants had significantly higher height,stem diameter,root length,fresh weight,dry weight and chlorophyll content,significantly higher activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT),significantly lower malondialdehyde(MDA)content and relative conductivity ratio level,showing their better growth status,higher active oxygen radicals scavenging capacity and less cell injury. These results indicated that overexpression of ZmLTP3 gene could improve the salt tolerance in transgenic maize.

Key words: ZmLTP3 gene, Pollen tube pathway method, Transgenic maize, Salt tolerance

中图分类号: 

• Q78

• S513.03