摘要： AREB/ABFs转录因子家族基因主要参与干旱、高盐、低温等胁迫应答反应。为了获得具有较高耐盐水平的棉花新种质材料，通过农杆菌介导法将耐盐转录因子基因（GHABF4）导入陆地棉中棉35中，通过对转化植株的卡那霉素初步筛选及T₁、T₂、T₃目的基因PCR的分子检测，获得T₃转基因棉花纯合系。通过盐胁迫试验对5个T₃转基因棉花株系和非转基因棉花对照进行耐盐性分析。结果表明，在200 mmol/L NaCl胁迫下，与非转基因对照相比，5个转基因棉花株系株高提高2.5~4.4 cm，地上部分的鲜质量增加3.6%~11.8%；且抗氧化物酶SOD、POD、CAT活性以及叶绿素含量提高。在盐胁迫条件下，转GHABF4基因棉花表现出优良的生长和生理优势，转GHABF4基因能够提高棉花的抗盐能力。

关键词: 棉花, GHABF4转录因子, 转基因棉花, 耐盐性

Abstract: AREB/ABFs transcription factor family genes are mainly involved in drought,high salt,low temperature and other stress response. To obtain the high salt tolerance level of cotton germplasm. The salt tolerance of transcription factor gene of GHABF4 was transformated into cotton inbred Zhong 35 plant by Agrobacterium-mediated method. Transgenic plants in T₁,T₂ and T₃ were detected by kanamycin resistance screening,PCR.Pure transgenic lines were obtained from T₃.Salt tolerance analysis was carried out on 5 T₃ transgenic cotton lines and non transgenic cotton plants by salt stress test. The results showed that under 200 mmol/L NaCl stress,compared with non transgenic lines,5 transgenic cotton plant height increased from 2.5 to 4.4 cm,on the part of the fresh quality increased by 3.6%-11.8%,antioxidant enzyme SOD,POD,CAT activity and chlorophyll content were higher than the control. The results of this study showed that under salt stress,the transgenic GHABF4 cotton showed good growth and physiological advantages,and the transgenic GHABF4 gene could enhance the salt tolerance of cotton.

Key words: Cotton, GHABF4 transcription factor, Transgenic cotton, Salt tolerance

中图分类号: 

• S562.03

• Q78