一组新的小麦EST-SSR标记开发及其 在遗传图谱构建中的应用

doi:10.7668/hbnxb.20191046

摘要/Abstract

摘要： 为了丰富小麦功能分子标记的数量，为小麦功能基因的定位、比较基因组学、小麦起源和进化等方面的研究奠定基础。从前期利用小麦转录组数据鉴定出的8 389个EST-SSR序列中，随机选取585对引物得分大于95的标记进行分析，以川麦42和川农16杂交后自交获得的重组自交系群体为试验材料，利用新开发出的EST-SSR标记，并结合SSR标记和SRAP标记，构建了一张遗传图谱。结果表明，585对EST-SSR引物中有555对能在亲本川麦42和川农16中扩增出稳定清晰的条带，引物有效性为94.87%。其中有44对EST-SSR引物在川麦42和川农16中表现出明显的、稳定的多态性，多态性率为7.93%。所构建的遗传图谱中含有本次新开发的EST-SSR标记的连锁群共12个，由163个标记组成，包括17个EST-SSR标记，73个SSR标记和73个SRAP标记，覆盖小麦基因组长度1 551.5 cM，相邻标记之间的平均距离为13.11 cM。本研究丰富了小麦功能分子标记的数量，为小麦功能基因的定位、比较基因组学、小麦起源和进化等方面的研究奠定基础。

关键词: 小麦, EST-SSR标记, 遗传图谱, RIL

Abstract: In order to enrich the mumber of wheat functional molecular markers,lay the foundation for the research of wheat functional gene location,copmarative genomics,wheat origin and evolution. In previous studies,8 389 potential EST-SSRs were identified from wheat transcriptome data. In the present study,585 pairs of primers were randomly selected for further analysis from 8 389 EST-SSR primers with a primer score of more than 95 points. And a wheat genetic map for the recombinant inbred line population derived from Chuanmai 42×Chuannong 16 was constructed using these newly developed EST-SSR markers and combined with SRAP markers and SSR markers. The results showed that 555 of the 585 pairs of primers were able to amplify the stable and clear bands in Chuanmai 42 and Chuannong 16,and the primer efficacy was 94.87%. Of these,44 pairs of EST-SSRs exhibited obvious and stable polymorphisms in parents with a polymorphism rate of 7.93%. There were 12 linkage groups in the genetic map containing newly developed EST-SSR markers. And these 12 linkage groups were composed of 163 markers,including 17 EST-SSRs markers,73 SSR markers,and 73 SRAP markers.The average distance between adjacent markers of 13.11 cM,and covering wheat genome length of 1 551.5 cM. This study enriched the number of functional molecular markers in wheat and laid the foundation for the study of wheat functional genes localization,comparative genomics,wheat origin and evolution.

Key words: Wheat, EST-SSR marker, Genetic map, RIL

中图分类号:

Q37
S512.03

吕冰冰, 代畅, 彭正松, YAMAMOTO Naoki, 吴一超, 魏淑红, 杨在君. 一组新的小麦EST-SSR标记开发及其在遗传图谱构建中的应用[J]. 华北农学报, 2020, 35(4): 57-63. doi: 10.7668/hbnxb.20191046.

LV Bingbing, DAI Chang, PENG Zhengsong, YAMAMOTO Naoki, WU Yichao, WEI Shuhong, YANG Zaijun. Development of a New Set of EST-SSR Markers and Their Application in Genetic Map Construction in Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(4): 57-63. doi: 10.7668/hbnxb.20191046.

http://www.hbnxb.net/CN/Y2020/V35/I4/57

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