转马铃薯<i>StPR1</i>基因烟草抗病性及生理特性分析

doi:10.7668/hbnxb.20190650

摘要/Abstract

摘要： 为明确马铃薯StPR1基因在抗病中的作用，构建了植物表达载体pBI121-StPR1，并通过农杆菌转化法将StPR1基因导入烟草中，采用不同的致病菌对转基因烟草进行处理，对其抗病性及生理特性进行研究，包括细菌病害软腐病（胡萝卜软腐欧文氏菌、菊欧氏菌和胡萝卜软腐欧文氏菌马铃薯黑胫亚种）和青枯病（茄科雷尔氏菌）、真菌病害干腐病（接骨木镰孢、燕麦镰孢）致病菌。结果表明，接种致病菌后，转基因型烟草和野生型烟草叶片上的病斑直径会随着病菌胁迫时间的延长而增大，并且转基因型烟草病斑直径明显小于野生型烟草；在生理特性分析中，野生型烟草和转基因烟草叶片中生理指标都会随着病菌胁迫时间的延长而呈上升趋势，但转基因烟草SOD、POD和CAT活性较野生型烟草均有不同程度的提高。抗病性及生理特性分析结果均表明，转基因烟草对于致病细菌和真菌均具有更强的抗性，说明StPR1基因在马铃薯植物抗病中起着重要的作用，为PR1基因在植物的抗病方面提供了理论基础。

关键词: 马铃薯, StPR1, 烟草, 遗传转化, 抗病分析

Abstract: A plant expression vector pBI121 -StPR1 was constructed to clarify the role of potato StPR1 gene in disease resistance, and introduce StPR1 gene into tobacco by Agrobacterium transformation method. The disease resistance and physiological characteristics of transgenic tobacco were investigated using different pathogen treatment, including bacterial disease soft rot (E. carotovora subsp. Carotovora Borgey, Ecc; E. chrysanthemi Burkholder. Atroseptica Dye, Ech; E. carotovora subsp. Mc Fadden et Dimock, Eca), bacterial wilt (Ralstonia solanacearum, RS) and fungal disease dry rot (F. sambucinum, F. avenaceum). The results showed that the diameter of lesions leaves increased with the prolongation of stress time in transgenic tobacco and wild type (WT) under the pathogen stress, and the diameter of transgenic tobacco lesions were significantly smaller than WT. In the physiological characteristics analysis, the physiological indexes of leaves increased with the prolongation of stress time in WT and transgenic tobacco, but the activities of SOD, POD and CAT in transgenic tobacco were all increased to different degrees compared WT. The results of disease resistance and physiological characteristics showed that transgenic tobacco had stronger resistance to pathogenic bacteria and fungi, indicating that the StPR1 gene played an important role in the disease resistance of potato, which could provide a theoretical basis for PR1 gene resistance in plants.

Key words: Solanum tuberosum, StPR1, Tobacco, Genetic transformation, Disease resistance analysis

中图分类号:

Q78
S532

贺付蒙, 李秀钰, 赵潇璨, 武佳文, 朱元芳, 周磊, 石奇海, 刘娣, 李凤兰. 转马铃薯StPR1基因烟草抗病性及生理特性分析[J]. 华北农学报, 2020, 35(3): 184-192. doi: 10.7668/hbnxb.20190650.

HE Fumeng, LI Xiuyu, ZHAO Xiaocan, WU Jiawen, ZHU Yuanfang, ZHOU Lei, SHI Qihai, LIU Di, LI Fenglan. Analysis of Disease Resistance and Physiological Characteristics of Transgenic Tobacco with Solanum tuberosum StPR1 Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(3): 184-192. doi: 10.7668/hbnxb.20190650.

http://www.hbnxb.net/CN/Y2020/V35/I3/184

参考文献

[1] 陈彦云, 曹君迈, 陈晓军, 任晓月, 刘喜平, 丁红瑾. 我国马铃薯贮藏加工产业现状及其发展建议[J]. 保鲜与加工, 2011, 11(6):47-49. doi:10.3969/j.issn.1009-6221.2011.06.013.
Chen Y Y, Cao J M, Chen X J, Ren X Y, Liu X P, Ding H J. Present situation analysis and development suggestions on potato storage and processing industry of China[J]. Storage and Process, 2011, 11(6):47-49.
[2] Zhang H, Xu F, Wu Y, Hu H H, Dai X F. Progress of potato staple food research and industry development in China[J]. Journal of Integrative Agriculture, 2017, 16(12):2924-2932.
[3] 孙海军.东北地区马铃薯常见病害的发生及防治[J]. 吉林农业, 2016(15):92. doi:10.14025/j.cnki.jlny.2016.15.038.
Sun H J. Occurrence and control of common diseases of potato in Northeast China[J]. Jilin Agriculture, 2016(15):92.
[4] van Loon L C, Van Strien E A. The families of pathogenesis-related proteins, their activities, and comparative analysis of PR-1 type proteins[J]. Physiological and Molecular Plant Pathology, 1999, 55(2):85-97. doi:10.1006/pmpp.1999.0213.
[5] 刘纪元, 佟少明, 侯和胜. 植物病程相关蛋白研究进展[J]. 黑龙江信息科技, 2012(5):65. doi:10.3969/j.issn.1673-1328.2012.05.077.
Liu J Y, Tong S M, Hou H S. Advances in research on plant pathogenesis related proteins[J]. Heilongjiang Science and Technology Information, 2012(5):65.
[6] Nasser W, de Tapia M, Burkard G. Maize pathogenesis-related proteins:characterization and cellular distribution of 1,3-β-glucanases and chitinases induced by Brome mosaic virus infection or mercuric chloride treatment[J]. Physiological and Molecular Plant Pathology, 1990, 36(1):1-14. doi:10.1016/0885-5765(90)90087-E.
[7] Alexander D, Goodman R M, Gut-Rella M, Glascock C, Weymann K, Friedrich L, Maddox D, Ahl-Goy P, Luntz T, Ward E. Increased tolerance to two oomycete pathogens in transgenic tobacco expressing pathogenesis-related protein 1a[J]. Proceedings of the National Academy of Sciences of the United States of America, 1993, 90(15):7327-7331. doi:10.1073/pnas.90.15.7327.
[8] Chun S C, Chandrasekaran M. Chitosan and chitosan nanoparticles induced expression of pathogenesis-related proteins genes enhances biotic stress tolerance in tomato[J]. International Journal of Biological Macromolecules, 2019, 125:948-954. doi:10.1016/j.ijbiomac.2018.12.167.
[9] Srivastava N, Gonugunta V K, Puli M R, Raghavendra A S. Nitric oxide production occurs downstream of reactive oxygen species in guard cells during stomatal closure induced by chitosan in abaxial epidermis of Pisum sativum[J]. Planta, 2009, 229(4):757-765. doi:10.1007/s00425-008-0855-5.
[10] Wang L, Guo Z H, Zhang Y B, Wang Y J, Yang G, Yang L, Wang R Y, Xie Z K. Isolation and characterization of two distinct Class Ⅱ PR4 genes from the oriental lily hybrid Sorbonne[J]. Russian Journal of Plant Physiology, 2017, 64(5):707-717. doi:10.1134/S1021443717050132.
[11] 李秀钰, 贺付蒙, 韩瑛琪, 赵潇璨, 武佳文, 朱元芳, 周磊, 石奇海, 冯哲, 李凤兰. 马铃薯StPR1 基因克隆及表达特异性分析[J]. 华北农学报, 2019, 34(2):66-71. doi:10.7668/hbnxb.201751433.
Li X Y, He F M, Han Y Q, Zhao X C, Wu J W, Zhu Y F, Zhou L, Shi Q H, Feng Z, Li F L. Cloning and expression characteristics of Solanum tuberosum StPR1 gene[J]. Acta Agriculturae Boreali-Sinica, 2019, 34(2):66-71.
[12] 胡重怡, 郑少清, 陈兴江, 陈丽莉, 程艳.烟草无菌苗培养前的种子消毒技术研究[J]. 中国烟草科学, 2007, 28(2):45-46,51. doi:10.3969/j.issn.1007-5119.2007.02.012.
Hu Z Y, Zheng S Q, Chen X J, Chen L L, Cheng Y. Methods for seed sterilization before aseptic tobacco seedling culture[J]. Chinese Tobacco Science, 2007, 28(2):45-46,51.
[13] 律凤霞. 根癌农杆菌介导外源基因转化烟草体系的优化[J]. 安徽农业科学, 2010, 38(19):10065-10066. doi:10.13989/j.cnki.0517-6611.2010.19.097.
Lü F X. Optimization for agrobacterium tumefaciens-mediated transformation system for exogenous genes of tobacco[J]. Journal of Anhui Agricultural Sciences, 2010, 38(19):10065-10066.
[14] Kitajima S, Sato F. Plant pathogenesis-related proteins:molecular mechanisms of gene expression and protein function[J]. The Journal of Biochemistry, 1999, 125(1):1-8. doi:10.1093/oxfordjournals.jbchem.a022244.
[15] Cameron R K,Paiva N L,Lamb C J,Dixon R A.Accumulation of salicylic acid and PR-1 gene transcripts in relation to the systemic acquired resistance (SAR) response induced by Pseudomonas syringae pv. tomato in Arabidopsis[J]. Physiological and Molecular Plant Pathology, 1999, 55(2):121-130. doi:10.1006/pmpp.1999.0214.
[16] Zhang G, Li Y M, Zhang Y, Dong Y L, Wang X J, Wei G R, Huang L L, Kang Z S. Cloning and characterization of a pathogenesis-related protein gene TaPR10 from wheat induced by stripe rust pathogen[J]. Agricultural Sciences in China, 2010, 9(4):549-556. doi:10.1016/S1671-2927(09)60128-0.
[17] 张计育, 渠慎春, 薛华柏, 高志红, 郭忠仁, 章镇. 湖北海棠病程相关蛋白MhPR8 基因的克隆与表达[J]. 中国农业科学, 2012, 45(8):1568-1575. doi:10.3864/j.issn.0578-1752.2012.08.012.
Zhang J Y, Qu S C, Xue H B, Gao Z H, Guo Z R, Zhang Z. Isolation and expression of pathogenesis-related protein gene MhPR8 from Malus hupehensis[J]. Scientia Agricultura Sinica, 2012, 45(8):1568-1575.
[18] Sarowar S, Kim Y J,Kim E N, Kim K D, Hwang B K,Islam R,Shin J S. Overexpression of a pepper basic pathogenesis-related protein 1 gene in tobacco plants enhances resistance to heavy metal and pathogen stresses[J]. Plant Cell Reports, 2005, 24(4):216-224. doi:10.1007/s00299-005-0928-x.
[19] Shi W N, Hao L L, Li J, Liu D D, Gou X Q, Li H. The Gossypium hirsutum WRKY gene GhWRKY39-1 promotes pathogen infection defense responses and mediates salt stress tolerance in transgenic Nicotiana benthamiana[J]. Plant Cell Reports, 2014, 33(3):483-498. doi:10.1007/s00299-013-1548-5.
[20] 乔子辰. 棉花黄萎病菌和Harpin_Xoo蛋白对转hpal_Xoo基因棉部分抗病生理指标的影响[D]. 乌鲁木齐:新疆农业大学, 2009. doi:10.7666/d.y1506399.
Qiao Z C.Part physiological resistance indexs of transgenic hpal_Xoo cotton interacting with Verticillium dahliae and Harpin_Xoo[D]. Urumqi:Xinjiang Agricultural University, 2009.

选择文件类型/文献管理软件名称

选择包含的内容

转马铃薯StPR1基因烟草抗病性及生理特性分析

Analysis of Disease Resistance and Physiological Characteristics of Transgenic Tobacco with Solanum tuberosum StPR1 Gene

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	张冬梅, 冯亚艳, 修志君, 杨春芳, 杜美娥, 李得宙, 张笑宇. *硅酸钠诱导的马铃薯抗黑痣病StWRKY11基因克隆及生物信息学分析*[J]. 华北农学报, 2022, 37(5): 194-203.
[2]	吉祥, 宋志成, 韦晓玲, 杨煜, 隋炯明, 郭宝太. 马铃薯卷叶病毒与S病毒重组双CP多克隆抗体的制备[J]. 华北农学报, 2022, 37(4): 212-219.
[3]	张之为, 范俊臣, 康立茹, 贾瑞芳, 田再民, 赵君. StRab5b基因对马铃薯花青素合成的影响[J]. 华北农学报, 2022, 37(4): 28-33.
[4]	李冬怡, 邓竹英, 梁大成. 基于sfGFP系统的嫁接体接口处的细胞质融合检测[J]. 华北农学报, 2022, 37(4): 151-157.
[5]	唐龙, 赵宇玮. *过表达PnSMO1.1基因显著增强裂叶牵牛耐盐性*[J]. 华北农学报, 2022, 37(2): 1-8.
[6]	和秋兰, 张航, 王正维, 万丽嫱, 海梅荣. 外源褪黑素对低温胁迫下马铃薯幼苗抗氧化系统的影响[J]. 华北农学报, 2022, 37(1): 103-111.
[7]	吴红红, 段学粉, 郭仰东, 张喜春. *转录因子SlMYB-related 2对番茄耐冷性的影响*[J]. 华北农学报, 2022, 37(1): 35-41.
[8]	张芮, 王腾飞, 张梅花, 张永胜, 杨昌钰, 陈志丕. 覆膜与补充灌溉对半干旱地区马铃薯产量及根际土壤微生物学特性的影响[J]. 华北农学报, 2021, 36(6): 132-140.
[9]	杨金睿, 王文倩, 王玥, 姚遥, 吉泽, 李俊逸, 陈斌, 肖关丽. 云南省4个主栽马铃薯品种叶片内生细菌多样性研究[J]. 华北农学报, 2021, 36(6): 178-188.
[10]	陈文涛, 潘根, 唐慧娟, 常丽, 李德芳, 赵立宁, 陈安国, 李建军. *红麻HcMS1基因的克隆、表达与功能分析*[J]. 华北农学报, 2021, 36(4): 64-74.
[11]	万丽嫱, 李光达, 和秋兰, 王正维, 张航, 海梅荣. 外源褪黑素对UV-B辐射下马铃薯光合、荧光特性的影响[J]. 华北农学报, 2021, 36(4): 116-123.
[12]	姜丽丽, 牟芮, 刘尚武, 张桂芝, 金光辉. amiRNA介导的兼抗PVX、PVY、PSTVd马铃薯植株获得及抗性鉴定[J]. 华北农学报, 2021, 36(3): 195-202.
[13]	李靓, 于卓, 于肖夏, 杨东升, 卢倩倩, 李景伟, 李佳奇, 刘宇飞. 6个马铃薯新品系的细胞学及SSR分析[J]. 华北农学报, 2021, 36(2): 74-80.
[14]	李佳皓, 谢敏秋, 万传银, 左瑞洁, 鲁黎明, 李立芹. *马铃薯转录因子StTCP13基因的原核表达及盐胁迫功能研究*[J]. 华北农学报, 2021, 36(2): 33-39.
[15]	胡月清, 王若仲, 黄志刚, 萧浪涛. *马铃薯转录因子基因StWRKY57的克隆及其蛋白原核表达*[J]. 华北农学报, 2021, 36(1): 10-17.

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

转马铃薯StPR1基因烟草抗病性及生理特性分析

Analysis of Disease Resistance and Physiological Characteristics of Transgenic Tobacco with Solanum tuberosum StPR1 Gene

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价