玉米蛋白激酶基因<em>ZmPti1-1</em>的cDNA克隆及其表达特性

doi:10.7668/hbnxb.2008.06.008

华北农学报 ›› 2008, Vol. 23 ›› Issue (6): 36-40. doi: 10.7668/hbnxb.2008.06.008

所属专题： 玉米；生物技术；

玉米蛋白激酶基因ZmPti1-1的cDNA克隆及其表达特性

边鸣镝^1,2,3, 吴忠义¹, 张秀海¹, 王永勤¹, 曹鸣庆¹, 黄丛林¹

1. 首都师范大学, 生命科学院, 北京, 100037;
2. 北京市农林科学院, 北京农业生物技术研究中心, 北京, 100097;
3. 吉林大学, 植物科学学院, 吉林, 长春, 130062

收稿日期:2008-10-16 出版日期:2008-12-28
通讯作者: 黄丛林(1969－)，男，四川仁寿人，副教授，博士，主要从事植物逆境分子生物学和菊花生物技术育种研究。
作者简介:边鸣镝(1971－)，女，吉林长春人，讲师，博士，主要从事植物逆境分子生物学研究。
基金资助:
国家“973”计划(2003CB114302)；国家自然科学基金(30400228；30700433)；北京市玉米育种创新平台项目(YZPT02-06)

cDNA Cloning of Maize Protein Kinase Gene ZmPtil-1 and Its Expression Characteristics

BIAN Ming-di^1,2,3, WU Zhong-yi¹, ZHANG Xiu-hai¹, WANG Yong-qin¹, CAO Ming-qing¹, HUANG Cong-lin¹

1. College of Life Science, Capital Normal University, Beijing 100037, China;
2. Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;
3. College of Plant Science, Jilin University, Changchun 130062, China

Received:2008-10-16 Published:2008-12-28

摘要/Abstract

摘要： 应用电子克隆的方法获得了玉米的一个Pti1(Pto－interacting 1)同源基因的全长cDNA，命名为ZmPti1-1(GenBank 接受号为EF1803)。推断ZmPti1-1蛋白含有362个氨基酸，分子量为39.00 kDa，pI为8.14，包含蛋白激酶催化结构域保守的11个亚结构域。在水杨酸 (Salicylic acid，SA)、甘露醇、氯化钠、脱落酸(Abscisic acid，ABA)和4℃低温的诱导下，ZmPti1-1在玉米幼苗叶片中的表达量明显上调。同时，在玉米不同发育时期、不同组织器官内，ZmPti1-1的表达水平也有很大差异，在子房中表达量最高，在雄蕊中检测不到表达。试验结果表明，ZmPti1-1是一个玉米类Pti1 基因，响应生物胁迫和非生物胁迫的诱导。

关键词: 玉米, Pti1, 生物胁迫, 非生物胁迫

Abstract: One full-length cDNA encoding a Ptil(Pto- interacting 1) homologue was isolated from maize through in silico cloning and named as ZmPtil-1.The predicted ZmPtil-1 protein has 362 amino acids with an est imated molecular mass of 39. 00 kDa and an isoelectric point of 8. 14 The putative kinase catalytic domain of ZmPtil-1 contains the 11 subdomains that are typical in protein kinase. ZmPtil-1 expression was up-regulated obviously by salicylic acid,mannitol, NaCl, absicic acid and low temperature(4) in young leaves of maize. Furthermore, ZmPtil-1 displayed different expression patterns in different growth stages and tissues and was most abundant in ovaries, but was undetectable in tassels. These results demonstrated that ZmPtil-1 is a maize Ptil- like gene and responds to biot ic and abiotic stress signals.

Key words: Zea mays, Ptil, Biotic stress, Abiotic stress

中图分类号:

Q945

边鸣镝, 吴忠义, 张秀海, 王永勤, 曹鸣庆, 黄丛林. 玉米蛋白激酶基因ZmPti1-1的cDNA克隆及其表达特性[J]. 华北农学报, 2008, 23(6): 36-40. doi: 10.7668/hbnxb.2008.06.008.

BIAN Ming-di, WU Zhong-yi, ZHANG Xiu-hai, WANG Yong-qin, CAO Ming-qing, HUANG Cong-lin. cDNA Cloning of Maize Protein Kinase Gene ZmPtil-1 and Its Expression Characteristics[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(6): 36-40. doi: 10.7668/hbnxb.2008.06.008.

http://www.hbnxb.net/CN/Y2008/V23/I6/36

参考文献

[1] Keen N T.Gene-for-gene complementarity in plant-pathogen interactions[J].Annu Rev Genet,1990,24:447-463.
[2] Dangl J L.The enigmatic avirulence genes of phytopathogenic bacteria[J].Curr Top Microbiol Immunol,1994,192:99-118.
[3] Hammond-Kosack K E,Jones J D A.Plant disease resistance genes[J].Annu Rev Plant Physiol Plant Mol Biol,1997,48:575-607.
[4] Hulbert S H,Webb C A,Smith S M,et al.Resistance gene complexes:evolution and utilization[J].Annu Rev Phytopathol,2001,39:285-312.
[5] Gabriel D W,Rolfe B G.Working models of specific recognition in plant-microbe interactions[J].Annu Rev Phytopathol,1990,28:365-391.
[6] Tang X,Frederick R D,Zhou J,et al.Initiation of plant disease resistance by physical interaction of AvrPto and Pto kinase[J].Science,1996,274:2060-2063.
[7] Scofield S R,Tobias C M,Rathjen J P,et al.Molecular basis of gene-for-gene specificity in bacterial speck disease of tomato[J].Science,1996,274:2063-2065.
[8] Zhou J,Loh Y T,Bressan R A,et al.The tomato gene Ptil encodes a serine/threonine kinase that is phosphorylated by Pto and is involved in the hypersensitive response[J].Cell,1995,83:925-935.
[9] Martin G B,Brommonschenkel S,Chunwongse L,et al.Map-based cloning of a protein kinase gene conferring disease in tomato[J].Science,1993b,262;1432-1436.
[10] Tian A G,Luo G Z,Wang Y J.Isolation and characterization of a Pti1 homologue from soybean[J].J Exp Bot,2004,396:535-537.
[11] Anthony R G,Khan S,Costa J,et al.The Arabidopsis protein kinase PTI1-2 is activated by convergent phosphatidic acid and oxidatice stress signaling pathways downstream of PDK1 and OXI1[J].Journal of Biology Chemistry,2006,281;37536-37546.
[12] Takahashi A,Agrawal K G,Yamazaki M,et al.Rice Pti1a negatively regulates RAR1-dependenr defense responses[J].The Plant Cell,2007,19:2940-2951.
[13] Zou H W,Wu Z Y,Yang Q,et al.Gene expression analyses of ZmPti1,encoding a maize Pti-like kinase,suggest a role in stress signaling[J].Plant Science,2006,171 (1):99-105.
[14] Herrmann M M,Pinto Sheena,Kluth J,et al.The Pti1-like kinase ZmPti1a from maize (Zea mays L.) co-localizes with callose at the plasma membrane of pollen and facilitates a competitive advantage to the male gametophyte[J].BMC Plant Biology,2006,6:1-22.
[15] Hanks S K,Quinn A M,Hunter T.The protein kinase family:conserved features and deduced phylogeny of the catalytic domains[J].Science,1988,241:42-52.
[16] Sessa G D,Ascenzo M,Martin G B.The major site of the Pti1 kinase phosphorylated by the Pto kinase is located in the activation domain and is required for Pto-Pti1 physical interaction[J].European Journal of Biochemistry,2000,267:171-178.
[17] Thomashow M F.Plant cold accumulation:freezing tolerance genes and regulatory mechanisms.Annu.Rev.Plant Physiol[J].Plant Molecular Biology,1999,50:571-599.
[18] Shinozaki K,Yamaguchi-Shinozaki K.Molecular responses to dehydration and low temperature:Differences and cross-talk between two stress signaling pathways[J].Current Opinion in Plant Biology,2000,3:217-223.

选择文件类型/文献管理软件名称

选择包含的内容

玉米蛋白激酶基因ZmPti1-1的cDNA克隆及其表达特性

cDNA Cloning of Maize Protein Kinase Gene ZmPtil-1 and Its Expression Characteristics

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	林静, 林建新, 张扬, 卢和顶, 陈山虎, 廖长见. 利用转录组解析鲜食玉米闽甜6855的耐寒机制[J]. 华北农学报, 2022, 37(5): 1-8.
[2]	金一锋, 高岩松, 王琦, 王萌萌, 赵迪, 熊毅, 陈阳. *草地早熟禾蛋白激酶SnRK2.4* 基因克隆及非生物胁迫响应分析**[J]. 华北农学报, 2022, 37(5): 9-15.
[3]	翟立超, 张丽华, 郑孟静, 吕丽华, 申海平, 姚海坡, 贾秀领. 夏玉米粒位效应对增密的响应及其碳氮代谢特征[J]. 华北农学报, 2022, 37(5): 97-104.
[4]	刘帅, 徐学欣, 赵金科, 曲文凯, 郝天佳, 孟繁港, 贾靖, 赵长星. 滴灌水肥一体化下施氮量与频次对夏玉米光合特性、保护酶活性及产量的影响[J]. 华北农学报, 2022, 37(5): 114-123.
[5]	王丽娟, 刘丹, 徐永清, 冯旭, 贺付蒙, 李爱雨, 王雪, 杨燕, 李翠婷, 袁强, 李凤兰. 寒地玉米秸秆不同腐熟时期的理化性状及微生物多样性分析[J]. 华北农学报, 2022, 37(5): 132-139.
[6]	付敬锋, 罗上轲, 程乙, 张军, 魏盛, 曾涛, 陈杜, 魏鹏程, 郑迎霞, 宋碧. 不同覆膜方式及施氮量对春玉米产量和氮素吸收利用的影响[J]. 华北农学报, 2022, 37(4): 158-168.
[7]	李强, 孔凡磊, 袁继超. 氮肥运筹对不同玉米品种氮素吸收、利用及田间氮平衡的影响[J]. 华北农学报, 2022, 37(4): 169-181.
[8]	赵思琪, 曲比伍合, 罗婷, 余学杰, 柯永培, 苟齐贤, 石海春. 玉米对灰斑病胁迫的生理生化响应[J]. 华北农学报, 2022, 37(4): 190-197.
[9]	张晓寒, 陈彦良, 马欣, 张珊珊, 韦善君. *沟叶结缕草ZmCOR410基因抗逆功能鉴定*[J]. 华北农学报, 2022, 37(4): 53-61.
[10]	王泳超, 燕博文, 曹红章, 王山聪, 马梦金, 张俊杰, 郭家萌, 王浩, 邵瑞鑫, 杨青华. DA-6与CCC复配对密植下玉米叶片光合及抗早衰能力的影响[J]. 华北农学报, 2022, 37(4): 90-102.
[11]	赵长江, 都梦翔, 宋巨奇, 徐尚缘, 贺琳, 徐晶宇, 杨克军, 李佐同. 玉米NRL基因家族鉴定与逆境表达分析[J]. 华北农学报, 2022, 37(4): 1-10.
[12]	闫留延, 李剑峰, 张世文, 张博, 王永芳, 张小梅, 祖超凡, 王振山, 桑璐曼, 何占祥, 贾小平, 董志平. *谷子SiPRR73基因的光温调控模式及非生物胁迫响应特性*[J]. 华北农学报, 2022, 37(4): 11-19.
[13]	张宗祥, 黄峥嵘, 吴雪凡, 刘楠楠, 李笑笑, 董召荣, 宋贺. 土壤酸化对玉米产量、氮代谢及相关基因表达的影响[J]. 华北农学报, 2022, 37(3): 94-103.
[14]	邰继承, 韩镁琪, 杨恒山, 张玉芹, 马金慧, 郭晓旭. 浅埋滴灌下尿素减量配施UAN对春玉米干物质积累及氮效率的影响[J]. 华北农学报, 2022, 37(3): 136-144.
[15]	刘建玲, 吴晶, 贾可, 廖文华, 吕英华, 马俊永. 冬小麦-夏玉米轮作区土壤磷与磷肥产量效应变化[J]. 华北农学报, 2022, 37(3): 104-111.

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

玉米蛋白激酶基因ZmPti1-1的cDNA克隆及其表达特性

cDNA Cloning of Maize Protein Kinase Gene ZmPtil-1 and Its Expression Characteristics

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价