摘要： 克隆了与草莓成熟有关的乙稀受体FaEtr2基因片段，为进一步研究FaEtr2基因功能并通过基因技术改善草莓贮运性能奠定基础。以千代田草莓成熟果实中分离到的基因组DNA为模板，经PCR扩增到1条约1.0 kb的特异片段，将该片段克隆到pGEM－T easy vector上经测序分析，基全长共1 09 bp，编号39个氨基酸残基。序列分析结果表明，该序列与Chandler－Etr2的cDNA序列同源性99%、氨基酸序列同源性为98%。

关键词: 草莓, 乙烯受体, 基因克隆, 序列分析

Abstract: In this study a ripening related ethylene receptor etr2 gene in strawberry fruits was cloned for the following-up research on gene funct ion and improving variet ies of strawberry through transgenic technology. A pair of primers were designed and synthesized based on the sequence reported of Chandler -Etr2. Using genome DNA extracted from Chinyoda strawberry mature fruits as template the specific PCR product of etr2 was obtained, then it was ligated to pGEM-T easy vector and sequenced. The sequencing data showed that the PCR product was 1 049 bp encoding 349 predicted amino acid residues. Comparison with the cDNA sequence from Chandler - etr2 indicated the homlogy was 99%, and amino acid identities were 98%.

Key words: Strawberry, Ethylene receptor, Gene clone, Sequence analysis

中图分类号: 

• S668.