Abstract:

To elucidate the molecular mechanism of prickle formation on the eggplant calyx,a genetic population was developed by crossing the homozygous prickly line 24C01(female parent)and the non-prickly line 24C02(male parent).In the F₂ generation,the distribution of calyx prickle density followed a normal distribution.Two extreme bulks were constructed by pooling DNA from 30 highly prickly and 30 completely non-prickly F₂ individuals.By combining BSA-seq with map-based cloning,the calyx prickle gene SmPL was fine-mapped to a 131.4 kb interval on chromosome 6.Among the 12 annotated genes within this region,only Smechr0602815.1,Smechr0602817.1,and Smechr0602821.1 showed differential expression between the two parental lines.Re-sequencing analysis further revealed a nonsynonymous mutation(G1066A)in Smechr0602815.1 in 24C02,resulting in a glutamate-to-lysine substitution,and a fragment deletion in Smechr0602821.1 in the same parent.Based on these sequence variations,kompetitive allele-specific PCR(KASP)markers linked to the non-prickly allele were developed.It was found that the markers exhibited a genotype-phenotype concordance rate exceeding 96% when tested across 100 diverse eggplant accessions.These results indicate that the developed KASP markers enable efficient early-selection for the non-prickly trait and can significantly accelerate the breeding of nonprickly eggplant varieties.

Key words: Eggplant, Calyx prickles, BSA-seq, Fine mapping, Molecular marker

CLC Number: 

• S641.1

• Q943.2植物基因工程