Abstract:

Chalcone synthase(CHS)is the initial and crucial enzyme in the flavonoid biosynthesis pathway,responsible for the synthesizing of metabolites such as flavones,flavonols,isoflavones,and anthocyanins,which play a vital role in enhancing plant stress resistance.In order to explore the role of CHS genes in the drought stress response of oat seedlings,it identified a CHS gene from the full-length transcriptome data of oats,named AsCHS.Gene cloning,bioinformatics analysis,subcellular localization,and expression pattern analysis were conducted.The results showed that the AsCHS gene encoded a protein composed of 398 amino acids and had a CHS family-specific tag sequence.This protein was hydrophobic and unstable.It was a non-transmembrane protein and was located in the nucleus and cytoplasm.Secondary structure prediction showed that AsCHS was mainly composed of α-helices and random coils.The analysis of the cis-acting elements within the promoter region revealed that the gene contained cis-elements associated with drought stress response and multiple hormone signaling pathways.Phylogenetic tree analysis showed that AsCHS was closely related to its counterparts in Lolium perenne,Poa annua,and Deschampsia antarctica.Subcellular localization indicated that the AsCHS protein was localized in the nucleus and cytoplasm.Compared with the control group,the expression pattern of AsCHS in oat seedlings under drought stress changed from fluctuating expression to incremental expression with different germination time,shifting from the highest expression level in roots to the highest in leaves,with significant differences observed in leaves expression.It laid a foundation for elucidating the function of AsCHS in the drought stress response of oats.

Key words: Oat, Chalcone synthase, Drought stress, Expression

CLC Number: 

• S512.6燕麦