Identification and Expression Pattern Analysis of CmPIPs Gene Family in Melon

doi:10.7668/hbnxb.20195142

Abstract

Abstract:

The identification and expression pattern analysis of CmPIPs gene family in melon could provide theoretical basis and support for further exploring the function of CmPIPs gene family and the genetic improvement of melon.TBtools,MEME,MEGA X and Plant-CARE tools were used to analyze the bioinformatics of CmPIPs,and the expression level of CmPIP2;7 in the pericarp of melon at different stages after pollination,and the expression level of each member of CmPIPs in different tissues and different concentrations of plant hormone treated young leaves were visualized in the software GraphPad Prism 10.The results showed that CmPIP2;7 and CsPIP2;8 had nearest kinship;the 12 members of the CmPIPs family were mainly distributed on chromosomes 1,3,4,5,9,10 and 11;except that CmPIP2;8 had 3 CDS regions,the other members had 4 CDS regions.The promoter regions of each member of CmPIPs had multiple cis-acting elements,hormone responsive elements,such as auxin,gibberellin,and abscisic acid.The expression level of CmPIP2;7 was significantly up-regulated during rapid development and maturity of melon fruit.Members of various families of CmPIPs were expressed in different tissues of melon.After treatment with 40.0 μmol/L auxin,the expression level of CmPIP2;4 was significantly up-regulated,while the expression level of CmPIP1;1,CmPIP 2;1,CmPIP2;2 and CmPIP2;3 were extremely significantly down-regulated,and when the concentrations of abscisic acid were 0.4,4.0 and 40.0 μmol/L,the expression level of CmPIP1;1,CmPIP2;1,CmPIP2;3,CmPIP2;9 were significantly down-regulated.After treatment with 44.640 μmol/L methyl jasmonate,the expression level of CmPIP2;1 and CmPIP2;5 were significantly down-regulated,while the expression level of CmPIP2;2,CmPIP2;3,CmPIP2;7 and CmPIP2;9 were significantly up-regulated.When the concentration of ethylene glycol was 4.0 mmol/L,the expression level of each member of CmPIPs was significantly up-regulated.The gene structure,sequence characteristics,evolutionary relationship and collinearity of CmPIPs gene family members were clarified,and their expression pattern were analyzed.

Key words: Melon, Gene family, Integral membrane protein, Plant hormone, Expression characteristics

HE Jiangjiang, JIN Lan, JIN Suriguga, LI Yong, HONG Yu, GAO Feng. Identification and Expression Pattern Analysis of CmPIPs Gene Family in Melon[J]. Acta Agriculturae Boreali-Sinica, 2025, 40(1): 53-62. doi: 10.7668/hbnxb.20195142.

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Figures/Tables 12

Tab.1 Sequences of primers used in RT-qPCR

基因名 Gene name	上游引物序列(5'—3') Forward primer sequence (5'—3')	下游引物序列(5'—3') Reverse primers sequence(5'—3')	大小/bp Size
CmGAPDH	ATCATTCCTAGCAGCACTGG	TTGGCATCAAATATGCTTGACCTG	278
CmPIP1;1	GGTGACTTTCGGGCTGTTTCT	CTTTGGTGTATCCCTCGCTCA	176
CmPIP1;2	TCTCCGCCACCGATGCTAA	AATGGTCATCCCAAGCGTGTT	189
CmPIP2;1	CGGTGTTCATGGTTCATTTGG	GCTTTTGCTGCTCCTGCTCTTA	199
CmPIP2;2	AGTAGCCAGTCCGACATCAAACA	TTCCACCGAAAGCCCAAGC	82
CmPIP2;3	ATTCGCCGTCAAGGATTACCA	TGCCACCAAATGCCCAAGC	233
CmPIP2;4	CCAAAGCAAAGACTACCAAGACC	CCCAAGCGATACCGAGAATG	212
CmPIP2;5	TAATAGACGGCGTGGAGATAAGG	ACATACAGGAAAAGCAGAGTCGC	85
CmPIP2;6	TGATGGAAGAAGCAATGTCAAGG	GAGAAGCGTGGCAACGAACT	124
CmPIP2;7	AAAGAAGTGACGGAAGAGGGACA	GCGATGAACTCAGCGATAAGG	131
CmPIP2;8	GGAGGGCACATAAATCCAGCAG	CCCACAATCTCAACAGCAAGG	224
CmPIP2;9	GATCATCCTCCGGCTCCCTT	CGGCAGAATCCTGACTTTGG	148
CmPIP2;10	ACAACAGCCAGACCGATACCA	GCACTAACGACACTTTACGAGCC	189

Fig.1 Gene structure and conservative motif analysis of PIPs family members in melon A.Conserved motif;B.Motif sequence micro label(The height of each letter indicates the frequency of occurrence of amino acids at that location);C.Gene structure.

Fig.2 Chromosomal mapping of CmPIPs gene family members

Fig.3 Synteny analysis of CmPIPs gene family members

Fig.4 Phylogenetic tree of PIPs family in melon,cucumber and watermelon

Fig.5 Promoter region cis-acting elements of various members of the CmPIPs gene family

Fig.6 Expression characteristics of CmPIP2;7 in the pericarp of melon at different days after pollination Different lowercase letters indicate significant differences at P<0.05 level. The results were 1 biological and 3 technical replicates. The same as Fig.7—11.

Fig.7 Expression characteristics of CmPIPs in different tissues

Fig.8 Expression characteristics of CmPIPs under auxin treatment

Fig.9 Expression characteristics of CmPIPs under abscisic acid treatment

Fig.10 Expression characteristics of CmPIPs under methyl jasmonate treatment

Fig.11 Expression characteristics of CmPIPs under ethylene treatment

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