Effects of Winter Sowing on Physiology and Biochemistry at Germination and Flowering of Winter/Spring Brassica napus L.

doi:10.7668/hbnxb.20194990

Abstract

Abstract:

In order to study the problem of strong winter/spring Brassica napus seed germination and flowering period under different winter sowing dates.Two strong winter rapeseeds and two spring rapeseeds provided by Gansu Agricultural University were used as materials.The experiment was carried out in the experimental field of Gansu Agricultural University from October 2022 to August 2023.The winter rapeseeds was carried out on October 11,2022.The winter/spring rapeseeds was sown every 20 days from December 10,2022,and the sowing ended on February 8,2023.The flowering period was recorded,and the germination seeds of winter rapeseed were sampled every 20 days to determine their physiological and biochemical characteristics and analyze the expression characteristics of vernalization genes(FLC,VRN2,FRI,FT).The results showed that the flowering period of winter/spring rape seeds was different by 22—34 days.The difference of flowering time between autumn sowing and spring sowing was 4—7 days.The flowering time of winter rapeseed in autumn sowing(October 11 th)was close to that of spring rapeseed under different winter sowing dates(December 10th,December 30th,January 19th,February 8th),and the flowering overlap time was as long as 15—20 days.With the delay of the sowing date,the relative expression levels of FLC,FRI and FT genes in germinating seeds of winter sowing were down-regulated.The relative expression of VRN2 gene was down-regulated in the early vernalization and up-regulated in the late vernalization.The activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT)and the contents of soluble protein(SP),gibberellin(GA3)and salicylic acid(SA)in germinating seeds were increased in the early vernalization,but those were decreased in the late vernalization.The contents of malondialdehyde(MDA)and abscisic acid(ABA)were increased in rapeseed germinating with the increase of vernalization time.

Key words: Brassica napus L., Asynchronous flowering period, Germinating seed, Vernalization, Physiological characteristic, Gene expression

GONG Yongjie, TIAN Haiyan, WEI Jiaping, CUI Junmei, WU Zefeng, DONG Xiaoyun, ZHENG Guoqiang, WANG Ying, WANG Xiaoxia, LIU Zigang. Effects of Winter Sowing on Physiology and Biochemistry at Germination and Flowering of Winter/Spring Brassica napus L.[J]. Acta Agriculturae Boreali-Sinica, 2024, 39(6): 106-114. doi: 10.7668/hbnxb.20194990.

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Figures/Tables 16

Tab.1 qRT-PCR primer sequences

引物名称 Primer name	引物序列 ( 5'—3') Primer sequence( 5'—3')
FLC	F: AGGAGAAGTTGCTGGAAGAGGAG
	R: CTTCGGCTCGCACAAGATTACTC
VRN2	F: ACGCCAGTTCTATCATTCTCACAG
	R: TCATCCACTTCATCTTCGCTATCTC
FRI	F: GCATCACCGCCGAGTCTCC
	R: CGCTCCACCTTCTCCGCTAC
FT	F: AAGAGTTGAGATTGGTGGAGAAGAC
	R: GTGAGGATTGCTAGGACTTGGAAC
Actin	F: ACGAGCAGGAGATGGAGACT
	R: CGAAGGCTGGAACAAGACCT

Fig.1 Overlapping time of flowering period of winter sowing winter/spring rape W1.Winter B.napus L.NTS221;W2.Winter B.napus L.NTS304;S1.Spring B.napus L.C05;S2.Spring B.napus L.C19;W1S1.NTS221-Vs-C05 flowering overlap time;W1S2.NTS221-Vs-C19 flowering overlap time;W2S1.NTS304-Vs-C05 flowering overlap time;W2S2.NTS304-Vs-C19 flowering overlap time.Different lowercase letters indicated that the difference between varieties with different sowing dates reached a significant level(P<0.05).The same as Fig.2—15.

Fig.2 Autumn sowing winter rape and spring sowing spring rape flowering overlap time

Fig.3 Autumn sowing winter rape and winter sowing spring rape flowering overlap time

Fig.4 Relative expression of FLC gene in germinating seeds of B.napus L.

Fig.5 Relative expression of VRN2 gene in germinating seeds of B.napus L.

Fig.6 Relative expression of FRI gene in germinating seeds of B.napus L.

Fig.7 Relative expression of FT gene in germinating seeds of B.napus L.

Fig.8 Effects of winter sowing on SOD activity in germinating seeds of B.napus L.

Fig.9 Effects of winter sowing on POD activity in germinating seeds of B.napus L.

Fig.10 Effects of winter sowing on CAT activity in germinating seeds of B.napus L.

Fig.11 Effects of winter sowing on MDA content in germinating seeds of B.napus L.

Fig.12 Effects of winter sowing on SP content in germinating seeds of B.napus L.

Fig.13 Effects of winter sowing on GA3 content in germinating seeds of B.napus L.

Fig.14 Effects of winter sowing on ABA content in germinating seeds of B.napus L.

Fig.15 Effects of winter sowing on SA content in germinating seeds of B.napus L.

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