Isolation and Genome-wide Evolutionary Analysis of Novel Variant Porcine parvovirus Type 1 in Shandong Province

doi:10.7668/hbnxb.20193540

Abstract

Abstract:

It conducted to analyze the biological characteristics of Porcine parvovirus type 1 (PPV1) mutant strains.The PPV was isolated and cultured from 67 aborted fetal tissue samples collected from pig farms in different regions of Shandong Province,and identified by electron microscopy and IFA test.DNAMAN V6 was used to demonstrate and compare the secondary structures of 5'UTR and 3'UTR,MEGA V7 was used for genetic evolutionary analysis,and finally the multi-step growth curve was used to compare the proliferation ability of the isolates in susceptible cells.Three strains were isolated and identified as PPV type 1 strains,named SDPV1,SDPV2 and SDPV3,with GenBank accession numbers ON924737,ON924738 and ON924739,respectively;the whole gene sequence lengths of the three isolates were basically the same,among which the 5'-UTR sequence was highly conserved and had a Y-shaped structure,while the 3' UTR had a U-shaped structure with some differences in individual bases;there were five non-synonymous mutation sites in the amino acid sequence of VP2,namely T20A,R82K,Q226E,D366N and K407N,which were found for the first time in domestic strains.The growth curve showed that the mutant strain had a relatively fast growth trend,with a 10-fold difference in the highest titer compared with the PPV-QN strain.It reported the genomic characteristics of PPV strains containing novel mutant loci.

Key words: Porcine parvovirus type 1, Mutant strain, Whole genome, Genetic variation

YU Yongle, YAO Yanzhu, ZHANG Ruihua, CHEN Chao, ZHAO Ting, SHAN Hu, HAN Xianjie. Isolation and Genome-wide Evolutionary Analysis of Novel Variant Porcine parvovirus Type 1 in Shandong Province[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(1): 232-238. doi: 10.7668/hbnxb.20193540.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.20193540

http://www.hbnxb.net/EN/Y2023/V38/I1/232

Figures/Tables 9

References 25

[1]	Cságola A, Lörincz M, Cadar D, Tombácz K, Biksi I, Tuboly T. Detection,prevalence and analysis of emerging Porcine parvovirus infections[J]. Archives of Virology, 2012, 157(6):1003-1010.doi:10.1007/s00705-012-1257-3. doi: 10.1007/s00705-012-1257-3 pmid: 22383055
[2]	Mayr A, Mahnel H. Cultivation of Hog cholera virus in pig kidney cultures with cytopathogenic effect[J]. Zentralbl Bakteriol Orig, 1964, 195(2):157-166.doi:10.1084/jem.74.5.427. doi: 10.1084/jem.74.5.427 pmid: 4953502
[3]	Streck A F, Canal C W, Truyen U. Molecular epidemiology and evolution of Porcine parvoviruses[J]. Infection, Genetics and Evolution, 2015, 36:300-306.doi:10.1016/j.meegid.2015.10.007. doi: 10.1016/j.meegid.2015.10.007 URL
[4]	Streck A F, Truyen U. Porcine parvovirus[J]. Current Issues in Molecular Biology, 2020, 37:33-46.doi:10.21775/cimb.037.033. doi: 10.21775/cimb.037.033 pmid: 31822635
[5]	Mészáros I, Olasz F, Cságola A, Tijssen P, Zádori Z. Biology of Porcine parvovirus(Ungulate parvovirus 1)[J]. Viruses, 2017, 9(12):393.doi:10.3390/v9120393. doi: 10.3390/v9120393 URL
[6]	Oh W T, Kim R Y, Nguyen V G, Chung H C, Park B K. Perspectives on the evolution of Porcine parvovirus[J]. Viruses, 2017, 9(8):196.doi:10.3390/v9080196. doi: 10.3390/v9080196 URL
[7]	Pinheiro de Oliveira T F, Fonseca A A Jr, Camargos M F, Macedo de Oliveira A, Lima N F, Freitas M E, de Oliveira Guedes E, Macedo de Azevedo I C, Pinto Cottorello A C, Heinemann M B. Porcine parvovirus as a contaminant in cell cultures and laboratory supplies[J]. Biologicals, 2016, 44(2):53-59.doi:10.1016/j.biologicals.2015.12.003. doi: 10.1016/j.biologicals.2015.12.003 pmid: 26811218
[8]	Zhang J L, Fan J H, Li Y, Liang S, Huo S S, Wang X, Zuo Y Z, Cui D, Li W Y, Zhong Z Y, Zhong F. Porcine parvovirus infection causes pig placenta tissue damage involving nonstructural protein 1 (NS1)-induced intrinsic ROS/mitochondria-mediated apoptosis[J]. Viruses, 2019, 11(4):389.doi:10.3390/v11040389. doi: 10.3390/v11040389
[9]	Cao L Y, Xue M, Chen J F, Shi H Y, Zhang X, Shi D, Liu J B, Huang L P, Wei Y W, Liu C M, Feng L. Porcine parvovirus replication is suppressed by activation of the PERK signaling pathway and endoplasmic reticulum stress-mediated apoptosis[J]. Virology, 2020, 539:1-10.doi:10.1016/j.virol.2019.09.012. doi: 10.1016/j.virol.2019.09.012 URL
[10]	Chen S B, Miao B C, Chen N N, Chen C Y, Shao T, Zhang X Z, Chang L L, Zhang X J, Du Q, Huang Y, Tong D W. SYNCRIP facilitates Porcine parvovirus viral DNA replication through the alternative splicing of NS1 mRNA to promote NS2 mRNA formation[J]. Veterinary Research, 2021, 52(1):73.doi:10.1186/s13567-021-00938-6. doi: 10.1186/s13567-021-00938-6
[11]	Streck A F, Bonatto S L, Homeier T, Souza C K, Gonçalves K R, Gava D, Canal C W, Truyen U. High rate of viral evolution in the capsid protein of Porcine parvovirus[J]. The Journal of General Virology, 2011, 92(Pt 11):2628-2636.doi:10.1099/vir.0.033662-0. doi: 10.1099/vir.0.033662-0 URL
[12]	Zimmermann P, Ritzmann M, Selbitz H J, Heinritzi K, Truyen U. VP1 sequences of German porcine parvovirus isolates define two genetic lineages[J]. The Journal of General Virology, 2006, 87(Pt 2):295-301.doi:10.1099/vir.0.81086-0. doi: 10.1099/vir.0.81086-0 URL
[13]	Tu M Y, Liu F, Chen S, Wang M S, Cheng A C. Role of capsid proteins in parvoviruses infection[J]. Virology Journal, 2015, 12:114.doi:10.1186/s12985-015-0344-y. doi: 10.1186/s12985-015-0344-y pmid: 26239432
[14]	de Souza A R, Yamin M, Gava D, Zanella J R C, Gatti M S V, Bonafe C F S, de Lima Neto D F. Porcine parvovirus VP1/VP2 on a time series epitope mapping:Exploring the effects of high hydrostatic pressure on the immune recognition of antigens[J]. Virology Journal, 2019, 16(1):75.doi:10.1186/s12985-019-1165-1. doi: 10.1186/s12985-019-1165-1
[15]	Li J X, Xiao Y Z, Qiu M, Li X S, Li S B, Lin H, Li X D, Zhu J Z, Chen N H. A systematic investigation unveils high coinfection status of Porcine parvovirus types 1 through 7 in China from 2016 to 2020[J]. Microbiology Spectrum, 2021, 9(3):e0129421.doi:10.1128/Spectrum.01294-21. doi: 10.1128/Spectrum.01294-21 URL
[16]	Díaz C, Celer V, Frébort I. The main DNA viruses significantly affecting pig livestock[J]. Journal of Veterinary Research, 2020, 65(1):15-25.doi:10.2478/jvetres-2021-0001. doi: 10.2478/jvetres-2021-0001 URL
[17]	Miłek D, Woz'niak A, Guzowska M, Stadejek T. Detection patterns of Porcine parvovirus(PPV) and Novel porcine parvoviruses 2 through 6 (PPV2-PPV6) in Polish swine farms[J]. Viruses, 2019, 11(5):474.doi:10.3390/v11050474. doi: 10.3390/v11050474 URL
[18]	Deka D, Barman N N, Deka N P, Batth B K, Singh G, Singh S, Agrawal R K, Mukhopadhyay C S, Ramneek. Sero-epidemiology of Porcine parvovirus,Circovirus,and Classical swine fever virus infections in India[J]. Tropical Animal Health and Production, 2021, 53(1):180.doi:10.1007/s11250-021-02587-5. doi: 10.1007/s11250-021-02587-5
[19]	Lagan Tregaskis P, Staines A, Gordon A, Sheridan P, McMenamy M, Duffy C, Collins P J, Mooney M H, Lemon K. Co-infection status of Novel parvovirus's(PPV2 to 4) with Porcine circovirus 2 in porcine respiratory disease complex and Porcine circovirus-associated disease from 1997 to 2012[J]. Transboundary and Emerging Diseases, 2021, 68(4):1979-1994.doi:10.1111/tbed.13846. doi: 10.1111/tbed.13846 URL
[20]	邹敏, 李陆梅, 宫晓, 王红, 杨旭兵, 范根成. 猪细小病毒PCR检测方法的建立与应用[J]. 中国动物检疫, 2016, 33(8):94-97.doi:10.3969/j.issn.1005-944X.2016.08.026. doi: 10.3969/j.issn.1005-944X.2016.08.026
	Zou M, Li L M, Gong X, Wang H, Yang X B, Fan G C. Establishment and application of PCR methods for Porcine parvovirus detection[J]. China Animal Health Inspection, 2016, 33(8):94-97.
[21]	Chen S B, Miao B C, Chen N N, Zhang X Z, Zhang X J, Du Q, Huang Y, Tong D W. A novel Porcine parvovirus DNA-launched infectious clone carrying stable double labels as an effective genetic platform[J]. Veterinary Microbiology, 2020, 240:108502.doi:10.1016/j.vetmic.2019.108502. doi: 10.1016/j.vetmic.2019.108502 URL
[22]	Bergeron J, H bert B, Tijssen P. Genome organization of the Kresse strain of Porcine parvovirus:Identification of the allotropic determinant and comparison with those of NADL-2 and field isolates[J]. Journal of Virology, 1996, 70(4):2508-2515.doi:10.1128/JVI.70.4.2508-2515.1996. doi: 10.1128/JVI.70.4.2508-2515.1996 pmid: 8642680
[23]	祖立闯, 谢金文, 王文秀, 李峰, 沈志强. 我国猪细小病毒病的流行现状及实验室诊断技术研究进展[J]. 养猪, 2016(6):125-128.doi:10.13257/j.cnki.21-1104/s.2016.06.043. doi: 10.13257/j.cnki.21-1104/s.2016.06.043
	Zu L C, Xie J W, Wang W X, Li F, Shen Z Q. Epidemiological status and research progress of laboratory diagnostic techniques in Porcine parvovirus disease[J]. Swine Production, 2016(6):125-128.
[24]	徐富权, 蒋运刚, 黄朝冲. 四川地区养猪场猪细小病毒病的病原检测及遗传进化分析[J]. 中国猪业, 2022, 17(1):77-80.doi:10.16174/j.issn.1673-4645.2022.01.014. doi: 10.16174/j.issn.1673-4645.2022.01.014
	Xu F Q, Jiang Y G, Huang C C. Pathogen detection and genetic evolution analysis of Swine parvovirus disease in pig farms in Sichuan[J]. China Swine Industry, 2022, 17(1):77-80.
[25]	郑艺, 徐伊璇, 彭文婧, 曹三杰, 颜其贵, 黄小波, 文翼平, 赵勤, 杜森焱, 伍锐. 猪细小病毒HNLY201301株遗传特性及其对小鼠的致病性研究[J]. 四川农业大学学报, 2022, 40(3):413-420.doi:10.16036/j.issn.1000-2650.202110078. doi: 10.16036/j.issn.1000-2650.202110078
	Zheng Y, Xu Y X, Peng W J, Cao S J, Yan Q G, Huang X B, Wen Y P, Zhao Q, Du S Y, Wu R. Genetic characteristics of Porcine parvovirus HNLY201301 strain and its pathogenicity to mice[J]. Journal of Sichuan Agricultural University, 2022, 40(3):413-420.

序号 No.	引物 Primer	引物序列(5'—3') Primer sequence(5'—3')	片段大小/bp Fragment size
1	Y1	F:AATCTTTAAACTGACCAACT	90
		R: ATGGTGACGTGTGACTGCCG
2	Y2	F: CTTCGGCAGTCACACGTCACCA	321
		R: TAAATACATAAGAGAATGC
3	C1	F: GGCTTCAAGATAATGCTCAA	1 900
		R: CAGGCTCTTATGTCGGTTTCTA
4	C2	F: ACAATGCAGCCAATGTGAACT	1 879
		R: ATTGAGTTGGTTTTGTAGGTA
5	C3	F: TCTGCAACAGGAAATGAATC	1 967
		R: GACATAAGGTCATATAAGTGTG
6	U1	F: AACCTAGACTACATGTTACAGC	674
		R: AGAGCCGCTTGGTTAGTCG
7	U2	F: GTATTCCTTTGAGTTAGTTG	158
		R: AACTAAACCAACCACACTTA

序号 No.	引物 Primer	引物序列(5'—3') Primer sequence(5'—3')	片段大小/bp Fragment size
1	Y1	F:AATCTTTAAACTGACCAACT	90
		R: ATGGTGACGTGTGACTGCCG
2	Y2	F: CTTCGGCAGTCACACGTCACCA	321
		R: TAAATACATAAGAGAATGC
3	C1	F: GGCTTCAAGATAATGCTCAA	1 900
		R: CAGGCTCTTATGTCGGTTTCTA
4	C2	F: ACAATGCAGCCAATGTGAACT	1 879
		R: ATTGAGTTGGTTTTGTAGGTA
5	C3	F: TCTGCAACAGGAAATGAATC	1 967
		R: GACATAAGGTCATATAAGTGTG
6	U1	F: AACCTAGACTACATGTTACAGC	674
		R: AGAGCCGCTTGGTTAGTCG
7	U2	F: GTATTCCTTTGAGTTAGTTG	158
		R: AACTAAACCAACCACACTTA

毒株 Strain	基因登录号 GenBank No.	收集时间 Collection date	国家 Country
BQ	EU790641	2006	中国
Taian	FJ853421	2008	中国
HN-2011	JX992846	2008	中国
GD2013	KX242359	2013	中国
HNLY201301	MF447833	2013	中国
SDQD20200424-830	MZ577027	2020	中国
JSYZ20170418-30	MZ577026	2017	中国
DJH23	MK092383	2015	中国
HuN 1	MH183298	2015	中国
China-XY	MK993540	2017	中国
PPV2010	JN872448	2010	中国
1242-2012	MH558678	2012	意大利
J-PPV	KF742500	2013	中国
27a	AY684871	2004	德国
15425	JN400520	2011	德国
Kresse	U44978	1996	加拿大
NADL-2	KF913345	2012	匈牙利

毒株 Strain	基因登录号 GenBank No.	收集时间 Collection date	国家 Country
BQ	EU790641	2006	中国
Taian	FJ853421	2008	中国
HN-2011	JX992846	2008	中国
GD2013	KX242359	2013	中国
HNLY201301	MF447833	2013	中国
SDQD20200424-830	MZ577027	2020	中国
JSYZ20170418-30	MZ577026	2017	中国
DJH23	MK092383	2015	中国
HuN 1	MH183298	2015	中国
China-XY	MK993540	2017	中国
PPV2010	JN872448	2010	中国
1242-2012	MH558678	2012	意大利
J-PPV	KF742500	2013	中国
27a	AY684871	2004	德国
15425	JN400520	2011	德国
Kresse	U44978	1996	加拿大
NADL-2	KF913345	2012	匈牙利

毒株 Strain	基因登录号 GenBank No.	基因组大小/bp Genome	5'-UTR	3'-UTR	NS1	NS2	NS3	VP1	VP2
SDPV1	ON924737	5 076	291	527	1 989	486	324	2 190	1 740
SDPV2	ON924738	5 078	291	529	1 989	486	324	2 190	1 740
SDPV3	ON924739	5 076	291	527	1 989	486	324	2 190	1 740

Please choose a citation manager

Content to export