Electrophoretic Analysis on 3 Kinds of Isozymes in Schizothorax waltoni

doi:10.7668/hbnxb.20192824

Abstract

Abstract:

This paper was to learn about the biochemical heredity characteristics and to enrich the research data about the germplasm resources of Schizothorax waltoni.Lactate dehydrogenase(LDH)in five kinds of tissues(heart,crystalline lens,muscle,liver and serum),malate dehydrogenase(MDH)in five kinds of tissues(heart,crystalline lens,muscle,liver and kidney)and alcohol dehydrogenase(ADH)isozymes in liver of Schizothorax waltoni were preliminarily studied by using the vertical polyacrylamide gel electrophoresis.The results showed that:the band numbers of LDH in heart,crystalline lens,muscle,liver and serum were 6-10,8-10,4-7,0-7 and 1-7,respectively.The band numbers of MDH isozymes in heart,crystalline lens,muscle,liver and kidney were 6-7,2-6,5-7,5 and 3-5,respectively.There was 3 ADH bands in the livers.LDH-C gene only expressed in liver.LDH and MDH showed tissue specificity and individual variations.The tissue specificity mainly reflected in band numbers and degree of isozyme activity.There were correlations between tissue specificity and its physiological functions.In order to adapt the unique habitant,the isozymes of Schizothorax waltoni showed individual variations.

Key words: Schizothorax waltoni, LDH, MDH, ADH, Tissue specificity, Individual variations

ZHANG Tao, ZHANG Lin, GAN Jinhua, HE Li. Electrophoretic Analysis on 3 Kinds of Isozymes in Schizothorax waltoni[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(S1): 366-372. doi: 10.7668/hbnxb.20192824.

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Figures/Tables 3

Fig.1 Electrophoretogram of LDH expressed in Schizothorax waltoni A-E.Electrophoretograms of LDH isozymes expressed in heart,crystalline lens,muscle,liver and serum,respectively.1-10.Zymograms of different individuals.

Fig.2 Electrophoretogram of MDH expressed in Schizothorax waltoni A-E.Electrophoretograms of MDH expressed in heart,crystalline lens,muscle,liver and kidney,respectively.1-4.Zymograms of different individuals.

Fig.3 Electrophoretogram of ADH expressed in liver of Schizothorax waltoni 1-10.Zymograms of different individuals.

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