Abstract:
In order to further improve the tolerance of BADH transgenic plants and obtain stronger stress-induced promotor,a regulatory sequence of 1 509 bp(Genebank:HM230828) was isolated from Atriplex micrantha,which grows in arid desert Xinjiang,according to the BADH cDNA sequences and refer to the methods of Chen.The transcription start site,which localized at 82 bases upstream of the start ATG,was predicted by soft-berry,a new intron(260 bp)was found.The functional elements were analysed by PLACE and PLANT CARE programm.The promotor contains lots of stress-induced elements,such as ABA response elements,salicylic acid,heat stock,low temperature,drought and wounding response elements,etc.Cu-stress,AM,Sugar,Anaerobic regulatory elements,GA response element,a number of light regulatory and transcription factor response elements(WRKY,CBF,MYB) was discovered firstly.We speculate that it is a new strong stress-induced promoter,we also constructed two plant expression vectors,BADH-P-a-3301 and BADH-P-b-3301.
Key words:
BADH gene,
Promotor,
Intron Clone
CLC Number:
REN Wei, WANG Zhi-feng, ZHOU Yan-chun, YU Hong-zhu, XU An-kai. Cloning and Construction of Expression Vectors of BADH Gene Promotor from Atriplex micrantha[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(S1): 1-5. doi: 10.7668/hbnxb.2011.S1.001.