Effects of Saline-alkali Stress on Physiology and Molecular Mechanism of Brassica napus L.

doi:10.7668/hbnxb.20192954

Abstract

Abstract:

In order to explore the effects of saline-alkali stress on the physiological and molecular mechanism of Brassica napus L.,using Huayouza 62 as experimental material.The seedlings of Brassica napus L.were treated with different concentrations of compound salt,compound alkali and compound saline-alkali solution.Physiological indexes such as seed germination rate,chlorophyll content,proline content,soluble sugar content and antioxidant enzyme activity in Brassica napus L.leaves were determined.The accumulation of betaine in rape leaves was determined by high performance liquid chromatography(HPLC).The key enzyme gene choline monooxygenase gene(CMO)in betaine synthesis pathway was analyzed by qRT-PCR technique.The results showed that the degree of damage to seed germination in artificially simulated saline-alkali solution of different concentrations was compound saline-alkali>alkali>salt.Low concentration saline-alkali solution promoted chlorophyll formation in rape leaves,while high concentration saline-alkali solution inhibited chlorophyll formation,saline-alkali stress significantly increased the contents of proline and soluble sugar,and the contents of proline and soluble sugar in high saline-alkali solution(YJ75,saline-alkali 75 mmol/L)for 21 d were 65.99 and 5.21 times higher than those in the control group,respectively,and the content of malondialdehyde was increased by saline-alkali stress.Saline-alkali stress significantly increased the activity of peroxidase(POD).Compared with the control group,the content of POD in high saline-alkali solution(YJ75)increased by 2.26 times after 21 d,and the content of POD reached the highest value on the 14th day after treatment with compound salt and compound alkali,however, the activity changes of superoxide dismutase (SOD) and catalase (CAT) were not obvious, and the role of species in the process of saline-alkali stress was low.Saline-alkali stress significantly increased the expression of key enzyme gene CMO,thus regulating the accumulation of betaine.In summary,the damage degree of saline-alkali stress to Brassica napus L. was compound saline-alkali > alkali > salt.Under high saline-alkali stress,Brassica napus would accumulate a large amount of betaine to reduce the damage.

Key words: Brassica napus L., Saline-alkali stress, Physiological indexes, HPLC, Betaine, qRT-PCR

LI Ban, LÜ Ying, YANG Mingxuan, SONG Ting, YU Fang, LIU Zhiwen. Effects of Saline-alkali Stress on Physiology and Molecular Mechanism of Brassica napus L.[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(3): 86-93. doi: 10.7668/hbnxb.20192954.

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Figures/Tables 13

Tab.1 Primer sequences

引物名称 Primer name	引物序列( 5'-3') Primer sequence( 5'-3')
PP2A-F1602	GTCAACAATCCGCACTACCTA
PP2A-R1711	ACCACGACGGGAAGAAAC
CMO-F674	CTGTATGGGGACCTTTTGTT
CMO-R799	CGCTCAACCTCCCTACAGA

Tab.2 The effect of saline-alkali stress on the seed germination

处理 Management		含盐量/(mmol/L) Salt content	发芽率/% Germination rate
CK	H₂O	0	100±0.00a
复合盐	Y25	25	98±0.94a
Compound	Y50	50	96±1.63a
salt	Y75	75	90±0.81b
复合碱	J25	25	68±2.44c
Compound	J50	50	18±2.16e
alkali	J75	75	4±1.41f
复合盐碱	YJ25	25	48±3.55d
Compound	YJ50	50	0±0.00g
saline-alkali	YJ75	75	0±0.00g

Fig.1 Effect of compound salt,compound alkali,compound saline-alkali on chlorophyll in leaves of Brassica napus L. Different lowercase letters indicate significant difference(P<0.05).The same as Fig.2-11.

Fig.2 Effect of compound salt,compound alkali,compound saline-alkali on the proline content in leaves of Brassica napus L.

Fig.3 Effect of compound salt,compound alkali,compound saline-alkali on the soluble sugar content in leaves of Brassica napus L.

Fig.4 Effect of compound salt,compound alkali,compound saline-alkali on malondialdehyde content in leaves of Brassica napus L.

Fig.5 Effect of salt stress on antioxidant enzyme activity in leaves of Brassica napus L.

Fig.6 Effect of alkali stress on antioxidant enzyme activity in leaves of Brassica napus L.

Fig.7 Effect of saline-alkali stress on antioxidant enzyme activity in leaves of Brassica napus L.

Fig.8 Effect of salt stress on relative expression of key enzyme genes in betaine synthesis

Fig.9 Effect of alkali stress on relative expression of key enzyme genes in betaine synthesis

Fig.10 Effect of saline-alkali stress on relative expression of key enzyme genes in betaine synthesis

Fig.11 The betaine content in leaves of Brassica napus L.

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