Cloning and Expression Analysis of Gossypium hirsutum Transcription Factor GhMYBPA1 Coding Gene

doi:10.7668/hbnxb.20191432

Abstract

Abstract: MYB transcription factor proteins are ubiquitous in plants and play an important role in biological and abiotic stresses. To explore the function of cotton MYB gene, the paper cloned a MYB gene from cotton leaves using homologous cloning technology, and analyzed its bioinformatics and expression under different stresses. The results showed that a new MYB transcription factor gene GhMYBPA1 (gene entry site XM_016869420) was successfully cloned from Gossypium hirsutum cv. Zhongmian 35, the full of cDNA was 825 bp in length with a 630 bp ORF, which encoded a 210 amino acids peptide. Bioinformatics analysis results showed that the molecular weight of GhMYBPA1 was 20.183 ku, GhMYBPA1 contained two conserved DNA-binding domains at N-terminal, which belonged to R2R3-MYB transcription factors. Amino acid homology analysis showed that GhMYBPA1 had higher identification with GaMYB12-like from Gossypium arboreum. Based on qRT-PCR analysis, GhMYBPA1 was constitutively expressed in cotton roots, stems, leaves, and it was dominantly expressed in flowers and then was leaves. Moreover, the results of plant treated with various stresses showed that the expression of GhMYBPA1 gene changed under high salt, low temperature and drought stress, it was suggested that GhMYBPA1 might play an important regulatory role in the abiotic stress process of cotton. The results could lay a theoretical foundation for further researches on the function of GhMYBPA1 gene.

Key words: Gossypium arboreum, GhMYBPA1, Bioinformatics analysis, Transcription factor, Abiotic stress, Functional analysis

CLC Number:

Q78
S562.03

ZHANG Anhong, ZHAO Zhansheng, WANG Zhian, XIAO Juanli, LIU Yuan, LUO Xiaoli. Cloning and Expression Analysis of Gossypium hirsutum Transcription Factor GhMYBPA1 Coding Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(6): 9-14. doi: 10.7668/hbnxb.20191432.

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