Characterization on TaMAPKK3,a Mitogen-activated Protein Kinase Gene in Wheat,in Mediating Plant Pi Deprivation Tolerance

doi:10.7668/hbnxb.20191184

Abstract

Abstract: In order to explore the wheat MAPK gene in improving the plant low-Pi stress tolerance,the molecular characterization of TaMAPKK3 was analyzed through bioinformatics technology. In addition,the expression pattern of TaMAPKK3 upon low-P treatment was evaluated;the target gene was integrated downstream of the promoter binary expression vector pCAMBIA3301 and subjected to tobacco transformation using Agrobacterium -mediated method. The tobacco lines overexpressing TaMAPKK3 were detected by qRT-PCR; using the transgenic tobacco as materials,based on measurements of growth traits,and physiological parameters and phosphorus content in plants,the gene function in mediating plant low-P stress capacity was identified.The results indicated that TaMAPKK3 contained an open reading frame of 1 572 bp,which encoded 523 amino acids with molecular weight 58.508 ku and isoelectric point 5.72. The encoded protein contained a typical domain shared by the MAPKKs family proteins and a nuclear transfer factor domain (NTF2) at the C-terminal position. The results suggested that it belonged to the B subgroup of the MAPKKs family. Phylogenetic analysis showed that TaMAPKK3 had a similar evolutionary pathway with TuMAPKK6 of Urartu wheat. Compared with that under normal P condition,the expression level of TaMAPKK3 was increased under low-P treatment and the transcriptional abundance was the highest at 27 h after treatment. The transcripts of the tested gene were decreased under the normal P recovery treatment and restored to its original amounts at 27 h after the recovery treatment. Compared with wild type (WT),the tobacco lines overexpressing TaMAPKK3significantly increased on the growth traits,fresh weight,dry matter accumulation and the root development,and displayed improved photosynthetic function and the phosphorus accumulation. Together,the results revealed that TaMAPKK3 responds to low-P stress at the transcription level,whose overexpression enhances the plant adaptation to P deprivation condition.

Key words: Wheat, TaMAPKK3 gene, Low-P stress, P uptake, Root system establishment

CLC Number:

Q78
S512.03

SONG Wenteng, MA Zifei, SHI Meihua, BAI Jinghua, LU Wenjing, XIAO Kai. Characterization on TaMAPKK3,a Mitogen-activated Protein Kinase Gene in Wheat,in Mediating Plant Pi Deprivation Tolerance[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2020, 35(5): 11-16. doi: 10.7668/hbnxb.20191184.

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