Bioinformatics and Expression Analysis of Three Dof Transcription Factors in Soybean

doi:10.7668/hbnxb.20190032

Abstract

Abstract: Dof transcription factors play an important role in plant response to abiotic stress. In order to investigate the Dof transcription factors of soybean involved in the response to abiotic stress, the bioinformatics analysis were performed on three Dof genes(GmDof2.1,GmDof3.1 and GmDof4.6) from soybean, which were located on different chromosomes. The length of the three Dof proteins ranged from 212 to 305 amino acid residues, all of which had a conserved Dof domain. They were mainly located in the nucleus and contained different amounts of phosphorylation sites. Promoter sequence analysis showed that GmDof2.1 promoter contained three cis-acting elements related to stress and hormone(ARE, DRE1 and MBS), GmDof3.1 promoter contained six cis-acting elements related to stress and hormone(ABRE, ARE, CGTCA-motif, TGACA-motif, W-box and WUN-motif), and GmDof4.6 promoter contained seven cis-acting elements related to stress and hormone(ABRE, ARE, CGTCA-motif, GARE-motif, MBS, TGACG-motif and WUN-motif). The results of Real-time fluorescence quantitative PCR showed that all three Dof genes could respond to the stresses of high salt, drought, low temperature and high temperature in varying degrees. GmDof2.1 and GmDof3.1 had the highest expression in roots, and GmDof4.6 had the highest expression in stems. It was inferred that the three Dof transcription factors might play a transcriptional regulatory role in soybean response to abiotic stresses.

Key words: Soybean, Dof transcription factor, Abiotic stress, Expression analysis

CLC Number:

S562.03
Q78

ZHAI Ying, QIU Shuang, ZHANG Jun, LIU Chunyu, ZHAO Yan, LI Shanshan, ZHANG Meijuan, QIU Zengcheng, REN Weiwei. Bioinformatics and Expression Analysis of Three Dof Transcription Factors in Soybean[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(6): 14-19. doi: 10.7668/hbnxb.20190032.

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