Bioinformatics and Expression Analysis of Cytokinin Receptor TaHK1 in Wheat

doi:10.7668/hbnxb.201751552

Abstract

Abstract: Cytokinin plays an important role in plant development and response to abiotic stresses,such as salinity and drought. To study the function of wheat cytokinin receptor gene, the Arabidopsis cytokinin receptor gene AHK4 (At2g01830)was used as the reference sequence to search the wheat genome, and the highest homologous gene TRIAE_CS42_4DS_TGACv1_362760_AA1181940(EnsemblPlants)was obtained, named as TaHK1. Bioinformatics analysis showed that TaHK1 gene was located on 4D chromosome of wheat, which encoded 996 amino acids. The relative molecular weight of TaHK1 was 109.70 ku, and the isoelectric point was 5.71. The secondary structure of the protein was composed of alpha helix, random coil, extended strand and beta turn. Subcellular localization predicted that TaHK1 was more likely located in the cell membrane and it contained two transmembrane domains. Protein conservative domain analysis showed that TaHK1 owned all the domains of a typical cytokinin receptor and it contained 87 phosphorylation sites. The expression analysis showed that TaHK1 was widely expressed in various tissues, such as root, stem, leaf, stamen and spikelet. In addition, the expression of TaHK1 could be induced by low phosphorus stress and a variety of pathogen infection, but inhibited by drought stress. It is presumed that TaHK1 might play an important role in the growth and development of wheat, and in resistance to drought stress and infection of pathogenic bacteria.

Key words: Wheat, Cytokinin receptor, TaHK1, Bioinformatics analysis, Gene expression

CLC Number:

S512.1
Q78

SUN Lijing, ZHAO Hui, Lü Liangjie, ZHANG Yingjun, HU Mengyun, LIU Qian, LI Hui. Bioinformatics and Expression Analysis of Cytokinin Receptor TaHK1 in Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(4): 75-82. doi: 10.7668/hbnxb.201751552.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.201751552

http://www.hbnxb.net/EN/Y2019/V34/I4/75

References

[1] Schaller G E, Bishopp A, Kieber J J. The yin-yang of hormones:cytokinin and auxin interactions in plant development[J]. Plant Cell, 2015, 27(1):44-63. doi:10.1105/tpc.114.133595.
[2] Bielach A, Hrtyan M, Tognetti V B. Plants under stress:involvement of auxin and cytokinin[J]. Int J Mol Sci, 2017,18(7):1427. doi:10.3390/ijms18071427.
[3] Xu Y, Burgess P, Huang B. Transcriptional regulation of hormone-synthesis and signaling pathways by overexpressing cytokinin-synthesis contributes to improved drought tolerance in creeping bentgrass[J]. Physiol Plant, 2017, 161(2):235-256. doi:10.1111/ppl.12588.
[4] Li W, Herrera-Estrella L, Tran L P. The Yin-yang of cytokinin homeostasis and drought acclimation/adaptation[J]. Trends Plant Sci, 2016, 21(7):548-550. doi:10.1016/j.tplants.2016.05.006.
[5] Zheng C F, Zhu Y J, Wang C Y, Guo T C. Wheat grain yield increase in response to pre-anthesis foliar application of 6-Benzylaminopurine is dependent on floret development[J]. PLoS One, 2016, 11(6):e0156627. doi:10.1371/journal.pone.0156627.
[6] Mok D W, Mok M C. Cytokinin metabolism and action[J]. Annu Rev Plant Physiol Plant Mol Biol, 2001, 52:89-118. doi:10.1146/annurev.arplant.52.1.89.
[7] Kundu A, DasGupta M. Silencing of putative cytokinin receptor histidine Kinase1 inhibits both inception and differentiation of root nodules in Arachis hypogaea[J]. Mol Plant Microbe Interact, 2018, 31(2):187-199. doi:10.1094/MPMI-06-17-0144-R.
[8] Wu C, Cui K H, Wang W C, Li Q, Fahad S, Hu Q H, Huang J L, Nie L X, Mohapatra P K, Peng S B. Heat-induced cytokinin transportation and degradation are associated with reduced panicle cytokinin expression and fewer spikelets per panicle in rice[J]. Front Plant Sci, 2017, 8:371. doi:10.3389/fpls.2017.00371.
[9] Heyl A, Schmülling T. Cytokinin signal perception and transduction[J]. Curr Opin Plant Biol, 2003, 6(5):480-488. doi:10.1016/S1369-5266(03)00087-6.
[10] Hwang I, Sheen J. Two-component circuitry in Arabidopsis cytokinin signal transduction[J]. Nature, 2001, 413(6854):383-389. doi:10.1038/35096500.
[11] Higuchi M, Pischke M S, Mähönen A P, Miyawaki K, Hashimoto Y, Seki M, Kobayashi M, Shinozaki K, Kato T, Tabata S, Helariutta Y, Sussman M R, Kakimoto T. In planta functions of the Arabidopsis cytokinin receptor family[J]. Proc Natl Acad Sci USA, 2004, 101(23):8821-8826. doi:10.1073/pnas.0402887101.
[12] Nishimura C, Ohashi Y, Sato S, Kato T, Tabata S, Ueguchi C. Histidine kinase homologs that act as cytokinin receptors possess overlapping functions in the regulation of shoot and root growth in Arabidopsis[J]. Plant Cell, 2004, 16(6):1365-1377. doi:10.1105/tpc.021477.
[13] Riefler M, Novak O, Strnad M, Schmülling T. Arabidopsis cytokinin receptor mutants reveal functions in shoot growth, leaf senescence, seed size, germination, root development, and cytokinin metabolism[J]. Plant Cell, 2006, 18(1):40-54. doi:10.1105/tpc.105.037796.
[14] Tran L S P, Urao T, Qin F, Maruyama K, Kakimoto T, Shinozaki K, Yamaguchi-Shinozaki K. Functional analysis of AHK1/ATHK1 and cytokinin receptor histidine kinases in response to abscisic acid, drought, and salt stress in Arabidopsis[J]. Proc Natl Acad Sci USA, 2007, 104(51):20623-20628. doi:10.1073/pnas.0706547105.
[15] 高嵩, 吕庆雪, 何欢, 张建新, 张志军, 宋广树, 刘伟. 玉米中绒毡层发育调控基因ZmUdt1 克隆的生物信息学分析[J]. 华北农学报, 2018, 33(1):52-59.doi:10.7668/hbnxb.2018.01.009.
Go S, Lü Q X, He H, Zhang J X, Zhang Z J, Song G S, Liu W. Bioinformatics analysis of ZmUdt1 gene in maize tapetum development[J]. Acta Agriculturae Boreali-sinica, 2018, 33(1):52-59.
[16] 孙丽静, 张哲, 刘茜, 胡梦芸, 张颖君, 吕亮杰, 李辉. 小麦组氨酸磷酸转运蛋白TaHP4 基因的克隆和表达分析[J]. 华北农学报, 2018, 33(2):14-19. doi:10.7668/hbnxb.2018.02.003.
Sn L J, Zhang Z, Liu Q, Hu M Y, Zhang Y J, Lü L J, Li H. Cloning and expression analysis of TaHP4 in wheat[J]. Acta Agriculturae Boreali-sinica, 2018, 33(2):14-19.
[17] Wilkins M R, Gasteiger E, Bairoch A, Sanchez J C, Williams K L, Appel R D, Hochstrasser D F. Protein identification and analysis tools on the ExPASy server[J]. Methods Mol Biol, 1999, 112:531-552. doi:10.1385/1-59259-584-7:531.
[18] Geourjon C, Deléage G. SOPMA:significant improvements in protein secondary structure prediction by consensus prediction from multiple alignments[J]. Comput Appl Biosci, 1995, 11(6):681-684. doi:10.1093/bioinformatics/11.6.681.
[19] Emanuelsson O, Nielsen H, Brunak S, von Heijne G. Predicting subcellular localization of proteins based on their N-terminal amino acid sequence[J]. Journal of Molecular Biology, 2000, 300(4):1005-1016. doi:10.1006/jmbi.2000.3903.
[20] Nielsen H. Predicting secretory proteins with SignalP[M]//Kihara D.Protein function prediction. New York:Humana Press, 2017:59-73. doi:10.1007/978-1-4939-7015-5_6.
[21] Bannai H, Tamada Y, Maruyama O, Nakai K, Miyano S. Extensive feature detection of N-terminal protein sorting signals[J].Bioinformatics,2002,18(2):298-305.doi:10.1093/bioinformatics/18.2.298.
[22] Krogh A, Larsson B, von Heijne G, Sonnhammer E L L. Predicting transmembrane protein topology with a hidden Markov model:application to complete genomes[J]. J Mol Biol, 2001, 305(3):567-580. doi:10.1006/jmbi.2000.4315.
[23] Letunic I, Bork P. 20 years of the SMART protein domain annotation resource[J]. Nucleic Acids Research, 2018, 46(D1):493-496. doi:10.1093/nar/gkx922.
[24] Blom N, Gammeltoft S, Brunak S. Sequence and structure-based prediction of eukaryotic protein phosphorylation sites[J]. J Mol Biol,1999,294(5):1351-1362.doi:10.1006/jmbi.1999.3310.
[25] Borrill P, Ramirez-Gonzalez R, Uauy C. expVIP:a customisable RNA-seq data analysis and visualisation platform[J]. Plant Physiology, 2016,170(4):2172-2186. doi:10.1104/pp.15.01667.
[26] Talla S K, Panigrahy M, Kappara S, Nirosha P, Neelamraju S, Ramanan R. Cytokinin delays dark-induced senescence in rice by maintaining the chlorophyll cycle and photosynthetic complexes[J]. J Exp Bot, 2016, 67(6):1839-1851. doi:10.1093/jxb/erv575.
[27] Raines T, Shanks C, Cheng C Y, McPherson D, Argueso C T, Kim H J, Franco-Zorrilla J M, López-Vidriero I, Solano R, Va ková R, Schaller G E, Kieber J J. The cytokinin response factors modulate root and shoot growth and promote leaf senescence in Arabidopsis[J]. Plant J, 2016, 85(1):134-147. doi:10.1111/tpj.13097.
[28] Sakakibara H. Cytokinin biosynthesis and regulation[J]. Vitam Horm, 2005, 72:271-287. doi:10.1016/S0083-6729(05)72008-2.
[29] Albacete A. Quantification of cytokinin levels and responses in abiotic stresses[M]//Dandekar T, Naseem M. Auxins and cytokinins in plant biology. New York:Humana Press, 2017:101-111. doi:10.1007/978-1-4939-6831-2_8.
[30] Verslues P E. ABA and cytokinins:challenge and opportunity for plant stress research[J]. Plant Mol Biol, 2016, 91(6):629-640. doi:10.1007/s11103-016-0458-7.
[31] Ha S, Vankova R, Yamaguchi-Shinozaki K, Shinozaki K, Tran L S. Cytokinins:metabolism and function in plant adaptation to environmental stresses[J]. Trends Plant Sci, 2012, 17(3):172-179. doi:10.1016/j.tplants.2011.12.005.
[32] Ding W, Tong H S, Zheng W J, Ye J, Pan Z C, Zhang B T, Zhu S H. Isolation, characterization and transcriptome analysis of a cytokinin receptor mutant Osckt1 in rice[J]. Front Plant Sci, 2017, 8:88. doi:10.3389/fpls.2017.00088.

Please choose a citation manager

Content to export