Cloning and Characterization of Soybean GmNAC23 Gene

doi:10.7668/hbnxb.201751084

Abstract

Abstract: NAC is a plant-specific transcription factor that plays an important role in regulating the development and response of abiotic and biotic stresses. In order to explore the function of soybean-related genes, a NAC gene GmNAC23 was cloned from soybean by PCR. The full length of GmNAC23 cDNA was 1 077 bp, including an ORF of 1 053 bp. This cDNA sequence encoded a polypepide of 350 amino acid residues with a predicted molecular mass of 39.483 ku and a basic isoelectric point of 7.08. Sequence analysis showed that the GmNAC23 genome contained three exons and two introns. Multiple sequence alignments showed that the GmNAC23 protein contained a highly conserved NAM domain. Phylogenetic analysis showed that the protein had the homology with the NAC protein of VrNAC, CaNAC and CarNAC. Transcriptional activation ability assay showed that the GmNAC23 could grow on SD (Trp-/His-/Ade-) deficient medium, indicating that the gene has transcriptional activation function in yeast. Tissue-specific expression profiles showed that GmNAC23 expressed almost in all detected tissues, and its expression level was relatively high in root and low in sam.The results indicated that the soybean GmNAC23 gene had a transcriptional activation function and was abundantly expressed in root, which laid a foundation for further study of the biological function of GmNAC23.

Key words: Soybean, GmNAC23, Cloning, Transcriptional activation

CLC Number:

Q78
S565.03

WANG Ping, YU Yuehua, BAI Yucui, GUO Kunpeng, KANG Jianan, NI Zhiyong. Cloning and Characterization of Soybean GmNAC23 Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(1): 46-53. doi: 10.7668/hbnxb.201751084.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.201751084

http://www.hbnxb.net/EN/Y2019/V34/I1/46

References

[1] Demorest Z L, Coffman A, Nicholas J B, Stoddard T J,Clasen B M, Luo S, Retterath A, Yabandith A, Gamo M E, Bissen J, Mathis L, Voytas D F, Zhang F.Direct stacking of sequence-specific nuclease-induced mutations to produce high oleic and low linolenic soybean oil[J]. BMC Plant Biology, 2016, 16:225. doi:10.1186/s12870-016-0906-1.
[2] Olsen A N, Ernst H A, Leggio L L, Skriver K.NAC transcription factors:structurally distinct, functionally diverse[J].Trends in Plant Science,2005,10(2):79-87.doi:10.1016/j.tplants.2004.12.010.
[3] Rushton D L, Tripathi P, Rabara R C, Lin J, Ringler P, Boken A K, Langum T J, Smidt L, Boomsma D D, Emme N J, Chen X, Finer J J, Shen Q J, Rushton P J.WRKY transcription factors:key components in abscisic acid signalling[J].Plant Biotechnology Journal,2012,10(1):2-11.doi:10.1111/j.1467-7652.2011.00634.x.
[4] Zhu J K.Salt and drought stress signal transduction in plants[J]. Annual Review of Plant Biology,2002,53:247-273.doi:10.1146/annurev.arplant.53.091401.143329.
[5] Nuruzzaman M, Sharoni A M, Kikuchi S.Roles of NAC transcription factors in the regulation of biotic and abiotic stress responses in plants[J].Frontiers in Microbiology,2013,4:248.doi:10.3389/fmicb.2013.00248.
[6] 陈娜, 蒋晶, 曹必好, 雷建军, 陈长明. 植物NAC转录因子功能研究新进展[J]. 分子植物育种, 2015,13(6):1407-1414.doi:10.13271/j.mpb.013.001407. Chen N, Jiang J, Cao B H, Lei J J, Chen C M. New progress in the study of plant NAC transcription factors[J]. Molecular Plant Breeding, 2015, 13(6):1407-1414.doi:10.13271/j.mpb.013.001407.
[7] Souer E, Vanhouwelingen A, Kloos D, Mol J, Koes R.The no apical meristem gene of petunia is required for pattern formation in embryos and flowers and is expressed at meristem and primordia boundaries[J].Cell,1996,85(2):159-170.doi:10.1016/S0092-8674(00)81093-4.
[8] Aida M, Ishida T, Fukaki H, Fujisawa H, Tasaka M. Genes involved in organ separation In Arabidopsis:an analysis of cup-shaped cotyledon mutant[J]. Plant Cell, 1997, 9:841-857. doi:10.1105/tpc.9.6.841.
[9] Hongbo S, Hongyan W, Xiaoli T. NAC transcription factors in plant multiple abiotic stress responses:progress and prospects[J]. Frontiers in Plant Science, 2015, 6:902. doi:10.3389/fpls.2015.00902.
[10] Puranik S, Sahu P P, Srivastava P S.NAC proteins:regulation and role in stress tolerance[J].Trends in Plant Science,2012,17(6):369-381.doi:10.1016/j.tplants.2012.02.004.
[11] Dagmara P S, Charlotte O, Per G, Skriver K. NAC Transcription factors in senescence:from molecular structure to function in crops[J]. Plants, 2015, 4(3):412-448. doi:10.3390/plants4030412.
[12] Yu X, Peng H, Liu Y, Zhang Y, Shu Y, Chen Q, Shi S, Ma L, Ma H, Zhang H. CarNAC2, a novel NAC transcription factor in chickpea (Cicer arietinum L.), is associated with drought-response and various developmental processes in transgenic arabidopsis[J]. Journal of Plant Biology, 2014, 57(1):55-66. doi:10.1007/s12374-013-0457-z.
[13] Hsin L S, Wang F H, Li J,Luo L, Lu S, Zhang X, Ma H, Ming F. The NAC family transcription factor OsNAP confers abiotic stress response through the ABA pathway[J]. Plant & Cell Physiology, 2014, 55(3):604. doi:10.1093/pcp/pct204.
[14] Fang Y, Liao K, Du H,Xu Y,Song H, Li X, Xiong L. A stress-responsive NAC transcription factor SNAC3 confers heat and drought tolerance through modulation of reactive oxygen species in rice[J]. Journal of Experimental Botany, 2015, 66(21):6803-6817. doi:10.1093/jxb/erv386.
[15] Jin H, Huang F, Cheng H, Song H, Yu D.Overexpression of the GmNAC2 gene,an NAC transcription factor,reduces abiotic stress tolerancein tobacco[J].Plant Molecular Biology Reporter,2013,31(2):435-442. doi:10.1007/s11105-012-0514-7.
[16] Quach T N, Tran L S, Valliyodan B, Nguyen H T, Kumar R, Neelakandan A K, Guttikonda S K, Sharp R E, Nguyen H T.Functional analysis of water stress-responsive soybean GmNAC003 and GmNAC004 transcription factors in lateral root development in arabidopsis[J].PLoS One,2014,9(1):e84886.doi:10.1371/journal.pone.0084886.
[17] 金杭霞. 大豆转录因子GmNAC2和GmNAC5功能验证[D]. 南京:南京农业大学, 2011. Jin H X. Functional verification of soybean transcription factors GmNAC2 and GmNAC5[D].Nanjing:Nanjing Agricultural University, 2011.
[18] 刘晓庆, 徐照龙, 许玲, 黄益洪, 何晓兰, 张大勇, 马鸿翔. 大豆GmNAC8基因克隆与原核表达[J]. 江苏农业学报, 2013, 29(4):734-737. doi:10.3969/j.issn.1000-4440.2013.04.007. Liu X Q, Xu Z L, Xu L, Huang Y H, He X L, Zhang D Y, Ma H X. Cloning and prokaryotic expression of soybean GmNAC8 gene[J]. Jiangsu Journal of Agricultural Sciences, 2013,29(4):734-737. doi:10.3969/j.issn.1000-4440.2013.04.007.
[19] 韩巧玲, 曹新有, 陈明, 陈耀锋, 李连城. 大豆转录因子GmNAC2a基因克隆及表达[J]. 西北植物学报, 2011, 31(1):8-13. doi:1000-4025(2011)01-0008-06. Han Q L, Cao X Y, Chen M, Chen Y F, Li L C. Isolation and expression pattern analysis of GmNAC2a from soybean[J]. Acta Botanica Boreali-Occidentalia Sinica, 2011,31(1):8-13. doi:1000-4025(2011)01-0008-06.
[20] Ni Z, Hu Z, Jiang Q, Zhang H.GmNFYA3, a target gene of miR169, is a positive regulator of plant tolerance to drought stress[J].Plant Molecular Biology,2013,82(1/2):113-129.doi:10.1007/s11103-013-0040-5.
[21] 刘辉, 王涛涛, 张俊红, 欧阳波, 李汉霞. 番茄NAC转录因子SlNAC80的克隆及表达分析[J]. 华北农学报, 2015, 30(1):77-83. doi:10.7668/hbnxb.2015.01.013. Liu H, Wang T T, Zhang J H, Ou Yang B, Li H X. Cloning and expression analysis of tomato NAC transcription factor SlNAC80[J]. Acta Agriculturae Boreali-Sinica, 2015,30(1):77-83. doi:10.7668/hbnxb.2015.01.013.
[22] 王国东, 李金晶, 白智伟, 关瑞攀, 杨野, 曲媛, 崔秀明, 刘迪秋. 三七NAC转录因子基因PnNAC1的克隆及表达特性分析[J]. 华北农学报, 2017, 32(4):91-97. doi:10.7668/hbnxb.2017.04.015. Wang G D, Li J J, Bai Z W, Guan R P, Yang Y, Qu Y, Cui X M, Liu D Q. Cloning and characterization of panax notogins NAC transcription factor gene PnNAC1[J]. Acta Agriculturae Boreali-Sinica, 2017,32(4):91-97. doi:10.7668/hbnxb.2017.04.015.
[23] Pinheiro G L, Marques C S, Costa M D, Reis P A, Alves M S, Carvalho C M, Fietto L G, Fontes E P.Complete inventory of soybean NAC transcription factors:Sequence conservation and expression analysis uncover their distinct roles in stress response[J].Gene,2009,444(1/2):10-23.doi:10.1016/j.gene.2009.05.012.
[24] Yu Y E, Ni Z Y, Chen Q J, Qu Y Y.The wheat salinity-induced R2R3-MYB transcription factor TaSIM confers salt stress tolerance in Arabidopsis thaliana[J].Biochemical and Biophysical Research Communications,2017,491(3):642-648.doi:10.1016/j.bbrc.2017.07.150.
[25] 李叶云, 王让剑, 王朝霞, 邓威威. 茶树鲜叶中β-半乳糖苷酶活性的测定[J]. 茶业通报, 2007(1):22-24. doi:1006-5768(2007)01-0022-03. Li S Y, Wang R J, Wang C X, Deng W W. Determination of β-galactosidase Activity in Fresh Leaves of Tea Tree[J]. Journal of Tea Business, 2007(1):22-24. doi:1006-5768(2007)01-0022-03.
[26] 邹嘉欣, 吕楠, 朱梦丽, 朱乾坤, 王万军. NAC家族生物信息学分析[J]. 生物技术通讯, 2015, 26(1):68-73. doi:10.3969/j.issn.1009-0002.2015.01.013. Zou J X, Lv N, Zhu M L, Zhu Q K, Wang W J. Bioinformatics analysis of NAC family[J]. Letters in Biotechnology, 2015,26(1):68-73. doi:10.3969/j.issn.1009-0002.2015.01.013.
[27] Meng Q C, Zhang C H, Gai J Y, Yu D Y.Molecular cloning, sequence characterization and tissue-specific expression of six NAC-like genes in soybean ( Glycine max (L.) Merr.)[J].Journal of Plant Physiology,2007,164(8):1002-1012.doi:10.1016/j.jptph.2006.05.019.
[28] 李伟, 韩蕾, 钱永强, 孙振元. 植物NAC转录因子的种类、特征及功能[J]. 应用与环境生物学报, 2011(4):596-606. doi:10.3724/SP.J.1145.2011.00596. Li W, Han L, Qian Y Q, Sun Z Y. Types,characteristics and functions of plant NAC transcription factors[J]. Chinese Journal of Applied & Environmental Biology, 2011(4):596-606. doi:10.3724/SP.J.1145.2011.00596.
[29] Tran L S, Quach T N, Guttikonda S K, Aldrich D L, Kumar R, Neelakandan A, Valliyodan B, Nguyen H T.Molecular characterization of stress-inducible Gm NAC genes in soybean[J].Molecular Genetics and Genomics,2009,281(6):647-664. doi:10.1007/s00438-009-0436-8.
[30] 倪志勇, 于月华, 陈全家, 曲延英. 大豆GmNAC115基因克隆及特征分析[J]. 大豆科学, 2016, 35(5):754-759. doi:10.11861/j.issn.1000-9841.2016.05.0754. Ni Z Y, Yu Y H, Chen Q J, Qu Y Y. Cloning and characterization of soybean GmNAC115 gene[J]. Soybean Science, 2016,35(5):754-759. doi:10.11861/j.issn.1000-9841.2016.05.0754.
[31] Hu H, Dai M, Yao J, Xiao B, Li X, Zhang Q, Xiong L.Overexpressing a NAM, ATAF, and CUC (NAC) transcription factor enhances drought resistance and salt tolerance in rice[J]. Proceedings of the National Academy of Sciences of the United States of America,2006,103(35):12987-12992.doi:10.1073/pnas.0604882103.
[32] Takasaki H, Maruyama K, Kidokoro S, Ito Y, Fujita Y, Shinozaki K, Yamaguchi-Shinozaki K, Nakashima K.The abiotic stress-responsive NAC-type transcription factor OsNAC5 regulates stress-inducible genes and stress tolerance in rice[J].Molecular Genetics and Genomics,2010,284(3):173-183.doi:10.1007/s00438-010-0557-0.
[33] 孟庆长. 大豆GmNAC和GmLFY转录因子编码基因的克隆,鉴定和种子性状的QTL定位研究[D]. 南京:南京农业大学, 2006. Meng Q C. Cloning,identification and QTL mapping of soybean GmNAC and GmLFY transcription factor encoding genes[D].Nanjing:Nanjing Agricultural University, 2006.

Please choose a citation manager

Content to export