Comparison of Physiological Characteristics and Differential Expression of FtNHX1 Gene among Twelve New Fagopyrum esculentum Varieties under Salt Stress

doi:10.7668/hbnxb.2018.05.020

Abstract

Abstract: In order to study the effects of salt stress on physiological characteristics and relative expression of FtNHX1 in Fagopyrum esculentum,twelve new Fagopyrum esculentum varieties (Pingqiao 2,Dingtianqiao 3,Chitianqiao 1,TQ11-3,T407-8,Tongqiao 2,Yuntianqiao 1,Meng 0825,Tianqiao 1307-179,Pingxuan 03-122,TQ11-6 and Chitianqiao 2) were used as experimental materials. The indexes of the seeds germination rate,seedlings fresh weight,roots vigor,leaf plasmalemma permeability,MDA content,SOD activity and relative expression of FtNHX1 in roots were determined under salt stress to compare the physiological characteristics and relative expression of FtNHX1 among twelve new Fagopyrum esculentum varieties. The results showed that the seeds germination rate and seedlings fresh weight of Dingtianqiao 3 and Chitianqiao 1 were decreased more than that of Pingqiao 2 and T407-8,while the seeds germination rate of Pingqiao 2 and T407-8 had no significant difference with the control,and had obviously promoting effects of salt stress on seedlings growth of the two Fagopyrum esculentum varieties. The leaf plasmalemma permeability and MDA content of Dingtianqiao 3 and Chitianqiao 1 were increased greatly,while the leaf plasmalemma permeability of Pingqiao 2 and T407-8 had no significant difference with the control,and the leaf MDA content of Pingqiao 2 and T407-8 increased to a lesser extent. The roots vigor and leaf SOD activity of Dingtianqiao 3 and Chitianqiao 1 were decreased greatly,while the roots vigors of Pingqiao 2 and T407-8 were decreased little,and the leaf SOD activities of Pingqiao 2 and T407-8 were increased significantly,indicated that had strong stress resistance. The relative expression of FtNHX1 gene of Dingtianqiao 3 was decreased significantly,while that of Pingqiao 2 and T407-8 were increased significantly,indicated that more salt tolerance. Fagopyrum esculentum varieties of Pingqiao 2 and T407-8 were determined salt tolerant varieties,while that of Dingtianqiao 3 and Chitianqiao 1 were determined salt sensitive varieties.

Key words: Fagopyrum esculentum, Salt stress, Physiological characteristics, Relative expression of FtNHX1 gene, Salt tolerance

CLC Number:

Q945.78
Q786

ZHANG Jiasong, LU Qihuan, LI Faliang, YANG Hongying, XU Chan, SONG Jinnan, WANG Yaqi, YANG Hongbing. Comparison of Physiological Characteristics and Differential Expression of FtNHX1 Gene among Twelve New Fagopyrum esculentum Varieties under Salt Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(5): 144-150. doi: 10.7668/hbnxb.2018.05.020.

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