Abstract: Genes encoding glycine-and proline-rich proteins (GPRPs) are widely distributed in higher plants,playing important roles in plant growth and development,and stress adaptation.In this study,to clone rice OsGPRPs,bioinformatics analysis combining RT-PCR and sequencing were performed.Complementary DNA (cDNA) sequences of three OsGPRPs encoding OsGPRPs with 197,180 and 170 amino acids,respectively,were obtained.Each OsGPRP encoded contained three motifs typical in plant GPRPs including an N-terminal XYPP repeat,a central hydrophobic region rich in alanine residues and a C-terminal HGK repeat.Online cis-acting regulatory element analysis against PlantCARE database were used to explore the function of these OsGPRPs.Plenty of putative cis-elements related to plant growth and hormone and stress responses were identified in the promoters of these OsGPRPs.To further understand the biological function of these OsGPRPs during the process of abiotic stress adaptation,quantitative real-time PCR (qRT-PCR) were used to analyze their responses under drought and cold stresses in rice seedlings.Responses of all three OsGPRPs to both drought and cold stresses were detected.Similar expression patterns with up-regulation of these OsGPRPs in response to drought were shown in different rice seedling tissues.However,obviously different patterns in response to low temperature were shown with elevated OsGPRP1 and OsGPRP3 but reduced OsGPRP2 expression levels.Results indicate important adaptive roles of these three OsGPRPs to abiotic stresses in rice.

Key words: Rice, OsGPRP, Gene cloning, Abiotic stress, Gene expression

CLC Number: 

• Q78

• S511.03