Cloning and Sequence Analysis of G-protein Beta-subunit Genes in Different Anastomosis Groups of Rhizoctonia solani

doi:10.7668/hbnxb.2017.05.002

Abstract

Abstract: To study the genetic difference and molecular evolution of G protein beta-subunit gene of Rhizoctonia solani. Full length of gDNA and cDNA sequences of G protein beta-subunit gene of 13 strains from 8 anastomosis groups (AGs) of Rhizoctonia solani were cloned and analyzed by homology-based cloning method. It showed that the number of intron and open reading frames were consistent in different isolates,which encoded 347 amino acids with 14 point mutations. Homology analysis showed that the homology between AGs of R.solani was high,and the similarity percent of isolates from the same intraspecific group was 100%. But there were genetic diverse among different AGs in homology. The phylogenetic analysis indicated that the G protein beta-subunit gene could effectively distinguish different AGs,and it also could differentiate all the AGs of R.solani from other fungi. It revealed the characteristic of G protein beta-subunit gene in different AGs from R.solani,and laid the foundation for the gene functional analysis and molecular detection of this fungus.

Key words: Rhizoctonia solani, Anastomosis groups, G protein, Gene cloning, Molecular evolution

CLC Number:

SHU Canwei, CAO Qiqi, ZHAO Mei, JIANG Shaofeng, ZHOU Erxun. Cloning and Sequence Analysis of G-protein Beta-subunit Genes in Different Anastomosis Groups of Rhizoctonia solani[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(5): 7-12. doi: 10.7668/hbnxb.2017.05.002.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2017.05.002

http://www.hbnxb.net/EN/Y2017/V32/I5/7

References

[1] 马亚男. 立枯丝核菌内切纤维素酶诱导寄主的防卫反应[D]. 泰安:山东农业大学,2016.
[2] Ogoshi A. Ecology and pathogenicity of anastomosis and intraspecific groups of Rhizoctonia solani Kuhn[J]. Annual Review of Phytopathology,1987,25(1):125-143.
[3] Carling D E,Kuninaga S,Brainard K A. Hyphal anastomosis reactions,rDNA-internal transcribed spacer sequences,and virulence levels among subsets of Rhizoctonia solani anastomosis group-2(AG-2) and AG-BI[J]. Phytopathology,2002,92(1):43-50.
[4] 肖勇. Ⅰ四川水稻立枯丝核菌的遗传分化与致病力研究;Ⅱ水稻立枯丝核菌G蛋白β亚基基因的克隆、表达与序列分析[D]. 雅安:四川农业大学,2008.
[5] Utsunomiya Y,Samejima C,Takayanagi Y,et al. Suppression of the rice heterotrimeric G protein β-subunit gene, RGB1,causes dwarfism and browning of internodes and lamina joint regions[J]. The Plant Journal:for Cell and Molecular Biology,2011,67(5):907-916.
[6] Sathyanesan A,Feijoo A A,Mehta S T,et al. Expression profile of G-protein βγ subunit gene transcripts in the mouse olfactory sensory epithelia[J]. Frontiers in Cellular Neuroscience,2013,7:84.
[7] Ma Y N,Chen M,Xu D B,et al. G-protein beta subunit AGB1 positively regulates salt stress tolerance in Arabidopsis[J]. Journal of Integrative Agriculture,2015,14(2):314-325.
[8] Subramaniam G,Trusov Y,Lopez-Encina C,et al. Type B heterotrimeric G protein γ-subunit regulates auxin and ABA signaling in tomato[J]. Plant Physiology,2016,170(2):1117-1134.
[9] Song N,Dai Q,Zhu B,et al. Gα proteins Gvm2 and Gvm3 regulate vegetative growth,asexual development,and pathogenicity on apple in Valsa Mali[J]. PLoS One,2017,12(3):e0173141.
[10] Guo L J,Yang L Y,Liang C C,et al. The G-protein subunits FGA2 and FGB1 play distinct roles in development and pathogenicity in the banana fungal pathogen Fusarium oxysporum f. sp cubense[J]. Physiological and Molecular Plant Pathology,2016,93:29-38.
[11] Cai Z D,Chai Y F,Zhang C Y,et al. The Gβ-like protein CpcB is required for hyphal growth,conidiophore morphology and pathogenicity in Aspergillus fumigatus[J]. Fungal Genetics and Biology,2015,81:120-131.
[12] Qi Z,Liu M,Dong Y,et al. The syntaxin protein (MoSyn8) mediates intracellular trafficking to regulate conidiogenesis and pathogenicity of rice blast fungus[J]. The New Phytologist,2016,209(4):1655-1667.
[13] Kasahara S,Nuss D L. Targeted disruption of a fungal G-protein beta subunit gene results in increased vegetative growth but reduced virulence[J]. Molecular Plant-microbe Interactions,1997,10(8):984-993.
[14] 李利,陈莎,毛涛,等. 丝状真菌G蛋白信号途径的研究进展[J]. 微生物学通报,2013,40(8):1493-1507.
[15] Ma H,Yanofsky M F,Meyerowitz E M. Molecular cloning and characterization of GPA1,a G protein alpha subunit gene from Arabidopsis thaliana[J]. Proceedings of the National Academy of Sciences of the United States of America,1990,87(10):3821-3825.
[16] Idnurm A,Howlett B J. Pathogenicity genes of phytopathogenic fungi[J]. Molecular Plant Pathology,2001,2(4):241-255.
[17] Ferrero-Serrano á,Assmann S M. The α-subunit of the rice heterotrimeric G protein,RGA1,regulates drought tolerance during the vegetative phase in the dwarf rice mutant d1[J]. Journal of Experimental Botany,2016,67(11):3433-3443.
[18] Yamagishi D,Otani H,Kodama M. G protein signaling mediates developmental processes and pathogenesis of Alternaria alternata[J]. Molecular Plant-microbe Interactions,2006,19(11):1280-1288.
[19] Wang P,Perfect J R,Heitman J. The G-protein beta subunit GPB1 is required for mating and haploid fruiting in Cryptococcus neoformans[J]. Molecular and Cellular Biology,2000,20(1):352-362.
[20] Nishimura M,Park G,Xu J R. The G-beta subunit MGB1 is involved in regulating multiple steps of infection-related morphogenesis in Magnaporthe grisea[J]. Molecular Microbiology,2003,50(1):231-243.
[21] Yu H Y,Seo J A,Kim J E,et al. Functional analyses of heterotrimeric G protein G alpha and G beta subunits in Gibberella zeae[J]. Microbiology,2008,154(2):392-401.
[22] Delgado-Jarana J,Martínez-Rocha A L,Roldán-Rodriguez R,et al. Fusarium oxysporum G-protein beta subunit Fgb1 regulates hyphal growth,development,and virulence through multiple signalling pathways[J]. Fungal Genetics and Biology,2005,42(1):61-72.

Please choose a citation manager

Content to export