Effect of Chaperone hfq Gene Deletion on Listeria monocytogenes Virulence and Biofilm Formation

doi:10.7668/hbnxb.2017.01.015

Abstract

Abstract: To understand the impacts of non-coding RNA (ncRNA) chaprone Hfq protein on LM's virulence,we compared LM- Δhfq with LM-SB5 in the following aspects:LD₅₀to Kunming mouse,amount in mouse liver and spleen as well as effects on pathology of mouse liver,spleen and kidney after inoculation.Hemolysis activity was determined by hemolysis activity assay experiment.Biofilm was prepared and measured to compare the capability of biofilm formation by using crystal violet staining.The results showed that LD₅₀ of LM-Δhfq to Kunming mouse was increased by 6.067 times than that of LM-SB5,indicating strain LM- Δhfq had significantly decreased virulence.LM-Δhfq amount in liver and spleen was significantly lower than that of LM-SB5 in the days of 2, 4, 5 after infection and extremely significant lower in the days of 3 after infection; pathological damages of liver,spleen and kidney of mouse infected LM- Δhfq was declined compared to that of LM-SB5 infection.Hemolysis activity of LM- Δhfq decreased 2 times than that of LM-SB5.The amount of biofilm formed by LM- Δhfq was attenuated.Taking together,the results indicated that ncRNA chaprone Hfq protein is crucial in regulating LM virulence and capability of biofilm formation.

Key words: Listeria monocytogenes, Non-coding RNA, hfq gene, Virulence

CLC Number:

S852

LU Haiting, QIAO Jun, MENG Qingling, PENG Yelong, CHEN Cheng, LIU Tianli, CAI Xuepeng, CHEN Chuangfu. Effect of Chaperone hfq Gene Deletion on Listeria monocytogenes Virulence and Biofilm Formation[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(1): 94-98. doi: 10.7668/hbnxb.2017.01.015.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2017.01.015

http://www.hbnxb.net/EN/Y2017/V32/I1/94

References

[1] 陆承平.兽医微生物学[M].北京:中国农业出版社,2010.
[2] Good J A,Andersson C,Hansen S,et al.Attenuating listeria monocytogenes virulence by targeting the regulatory protein PrfA[J].Cell Chemical Biology,2016,23(3):404-414.
[3] Kakoschke T,Kakoschke S,Magistro G,et al.The RNA chaperone Hfq impacts growth,metabolism and production of virulence factors in Yersinia enterocolitica[J].PLoS One,2014,9(1):e86113.
[4] Someya T,Baba S,Fujimoto M,et al.Crystal structure of Hfq from Bacillus subtilis in complex with SELEX-derived RNA aptamer:insight into RNA-binding properties of bacterial Hfq[J].Nucleic Acids Research,2012,40(4):1856-1867.
[5] Pandey S P,Winkler J A,Li H,et al.Central role for RNase YbeY in Hfq-dependent and Hfq-independent small-RNA regulation in bacteria[J].BMC Genomics,2014,15(1):121.
[6] Meng X,Meng X,Zhu C,et al.The RNA chaperone Hfq regulates expression of fimbrial-related genes and virulence of Salmonella enterica serovar Enteritidis[J].FEMS Microbiology Letters,2013,346(2):90-96.
[7] Mellin J R,Cossart P.The non-coding RNA world of the bacterial pathogen Listeria monocytogenes[J].RNA Biology,2012,9(4):372-378.
[8] Sievers S, Lund A, Menendez-Gil P, et al. The multicopy sRNA LhrC controls expression of the oligopeptide-binding protein OppA in Listeria monocytogenes[J]. RNA Biol, 2015, 12(9):985-997.
[9] Teschler J K,Zamorano-Sánchez D,Utada A S,et al.Living in the matrix:assembly and control of Vibrio cholerae biofilms[J].Nature Reviews Microbiology,2015,13(5):255-268.
[10] Sauer E.Structure and RNA-binding properties of the bacterial LSm protein Hfq[J].RNA Biology,2013,10(4):610-618.
[11] Mura C,Randolph P S,Patterson J,et al.Archaeal and eukaryotic homologs of Hfq:A structural and evolutionary perspective on Sm function[J].RNA Biology,2013,10(4):636-651.
[12] Yonekura K,Watanabe M,Kageyama Y,et al.Post-transcriptional regulator Hfq binds catalase HPII:crystal structure of the complex[J].PLoS One,2013,8(11):e78216.
[13] Régnier P,Hajnsdorf E.The interplay of Hfq,poly(A) polymerase Ⅰ and exoribonucleases at the 3' ends of RNAs resulting from Rho-independent termination:A tentative model[J].RNA Biology,2013,10(4):602-609.
[14] Wilusz C J,Wilusz J.Lsm proteins and Hfq:Life at the 3 end[J].RNA Biology,2013,10(4):592-601.
[15] Nielsen J S,Larsen M H,Lillebæk E M,et al.A small RNA controls expression of the chitinase ChiA in Listeria monocytogenes[J].PLoS One,2011,6(4):e19019.
[16] Sievers S,Sternkopf Lillebæk E M,Jacobsen K,et al.A multicopy sRNA of Listeria monocytogenes regulates expression of the virulence adhesin LapB[J].Nucleic Acids Research,2014,42(14):9383-9398.
[17] Christiansen J K,Larsen M H,Ingmer H,et al.The RNA-binding protein Hfq of Listeria monocytogenes:role in stress tolerance and virulence[J].Journal of Bacteriology,2004,186(11):3355-3362.
[18] Huang Y,Suo Y,Shi C,et al.Mutations in gltB and gltC reduce oxidative stress tolerance and biofilm formation in Listeria monocytogenes 4b G[J].International Journal of Food Microbiology,2013,163(2/3):223-230.
[19] Peng Y L,Meng Q L,Qiao J,et al.The regulatory roles of ncRNA Rli60 in adaptability of listeria monocytogenes to environmental stress and biofilm formation[J].Current Microbiology,2016,73(1):77-83.
[20] Zhou Q,Feng F,Wang L,et al.Virulence regulator PrfA is essential for biofilm formation in Listeria monocytogenes but not in Listeria innocua[J].Current Microbiology,2011,63(2):186-192.

Please choose a citation manager

Content to export