Expression of AtHDG11 in Transgenic Maize and Drought Resistance Identification

doi:10.7668/hbnxb.2016.06.010

Abstract

Abstract: The objective of this experiment is to study the production of transgenic maize with the resistance of drought stress.In this study,an expression vector containing drought resistance gene AtHDG11 was used to transform to maize elite inbred line Chang 7-2 by ultrasonic assisted Agrobacterium mediated pollen transformation method,and produced transgenic maize plants.Based on herbicid resistance,PCR analysis and transcriptional analysis,five transgenic maize lines were selected from a large number of transgenic lines.Subsequently,the inbred line Chang 7-2 was used as the nontransgenic control to analyze the drought resistance of transgenic plants.Physiological active substances and photosynthetic parameters were measured on plants at the seedling stage and 10-leaf stage in a drought stress treatment.The results showed that Pro content of transgenic maize was higher than that of the CK plants under drought stress conditions,while MDA content was lower.Both normal conditions and drought condition,photosynthetic rate and water use efficiency of transgenic lines were significantly higher than that of CK plants.And transpiration rate and stomatal conductance of transgenic lines were significantly lower than the control plants.Therefore,ultrasonic assisted Agrobacterium mediated pollen transformation method is feasible in the maize. AtHDG11 is a strong biotechnological candidate for use in efforts to produce a drought-resistance maize.This study will provide new germplasm resources and new transgenic method.

Key words: Maize, Drought resistance, AtHDG11, Transformation

CLC Number:

S332.4

NAN Zhirun, HOU Lei, LIU Xia, JIAO Xiongfei, JIAO Gaili, WU Shenjie. Expression of AtHDG11 in Transgenic Maize and Drought Resistance Identification[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(6): 63-67. doi: 10.7668/hbnxb.2016.06.010.

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http://www.hbnxb.net/EN/Y2016/V31/I6/63

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