Analysis of Sequences and Variation Sites of Fusarium Wilt Resistance Gene FOC1 in Brassica oleracea

doi:10.7668/hbnxb.2016.03.001

Abstract

Abstract: The aim of this study was to uncover the specific SNPs of fusarium wilt resistance gene FOC1 of Brassica oleracea, which will lay the foundation for the development of specific molecular markers in the future.Based on the identification of fusarium wilt resistance in 11 cabbage inbred lines,the variations of gene sequences and amino acid sequences of FOC1 and its alleles in 11 cabbage materials were analyzed.Results showed that there were 92 SNPs and 2 Indels between these sequences,including 18 specific SNPs related to fusarium wilt resistance,of which 86.11% were transition mutations and 13.89% were transversion mutations.The nucleotide substitutions of T/C (47.22%) and G/A (38.89%) showed higher mutation rates,while C/G and A/C accounting for about 13.89%.In addition,the four specific SNPs identified between the susceptible and resistant cabbage materials led to the variations of three amino acids.

Key words: Brassica oleracea, Fusarium wilt resistance, Specific SNP, Sequence analysis

CLC Number:

LEI Lei, MA Jian, LV Honghao, XIE Jianming, YU Jihua, KANG Jungen. Analysis of Sequences and Variation Sites of Fusarium Wilt Resistance Gene FOC1 in Brassica oleracea[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(3): 1-10. doi: 10.7668/hbnxb.2016.03.001.

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