Antifungal Activity of Chitinase on the Several Pathogen from Fruit & Vegetable in vitro

doi:10.7668/hbnxb.2014.04.027

Abstract

Abstract: Inhibitory effect of recombinant chitinase on the pathogen that caused postharvest decay of fruits and vegetables was studied to supply new method for the biological control of postharvest loss.The recombinant chitinase was used to investigate its antifungal activity against nine fungi in vitro.Results showed that the chitinase inhibited the growth of hypha in different degrees,the antifungal activity against Rhizopus stolonifer (Ehrenb.Et Fr.) Vuill ,Botrytis squamosa Walker ,Pythium aphanidermatum (Eds.) Fitzp,and Aspergillus niger van Tiegh was stronger than the Alternaria alternate and Fusarium roseum Link.Chitinase inhibited the hyphal extension and altered the growth pattern of hyphal tip.At the same time,the chitinase showed inhibitory effect on the spore germination of Rhizopus stolonifer, and this effect was more obvious with the increase of chitinase concentration.Inhibitory rate on the spore germination of Rhizopus stolonifer and Botrytis reached more than 91% when the concentration of chitinase was 0.40%.In conclusion,recombinant chitinase had significantly inhibitory effect on the growth of pathogen that caused postharvest decay of fruits and vegetables,and had the potential of development and application in the biological control of postharvest decay of fruits and vegetables.

Key words: Chitinase, Fruits and vegetables, Pathogene, Inhibitory effect

CLC Number:

TS255.3

YAN Rui-xiang, ZHANG Na, GUAN Wen-qiang, YU Jin-ze. Antifungal Activity of Chitinase on the Several Pathogen from Fruit & Vegetable in vitro[J]. journal1, 2014, 29(4): 155-159. doi: 10.7668/hbnxb.2014.04.027.

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