Effect of Puerarin on Expression of Inducible HSP70 in LLC-PK1 Cells

doi:10.7668/hbnxb.2014.03.007

Abstract

Abstract: This experiment was to study the effect of puerarin on the thermal stress induced HSP70 expression in pig renal tubular epithelial cell (LLC-PK1), and to selected the optimal concentration of puerarin for inducing HSP70 expression.The LLC-PK1 in its logarithmic growth period was selected and divided into 37 ℃ control group and 42 ℃ heat stress group.The heat stress group of cell was incubated with various concentrations (from 0.01-100 μmol/L) of puerarin before the heat stress treatment, and then the total RNA and protein of the cells were extracted and were detected by RT-PCR and Western Blot, respectively.The results showed that both thermal stress at 42 ℃ and puerarin with various concentrations significantly induced the HSP70 expression of LLC-PK1 (P<0.01), and HSP70 expression increased as the puerarin concentration increased in the culture medium.The relative expression of HSP70 in the heat stress group was the highest in the 10 μmol/L of puerarin group.The above results indicate that puerarin can induce HSP70 expression of LLC-PK1 under heat stress and this expressions increased as the puerarin concentration increased in the culture medium.

Key words: LLC-PK1, Puerarin, Heat stress, HSP70

CLC Number:

WANG Zhan-mei, ZHAO Yuan, CHEN Jian-wei, GUO Xiao-tong, CONG Xia, JIANG Zhong-ling, TIAN Wen-ru. Effect of Puerarin on Expression of Inducible HSP70 in LLC-PK1 Cells[J]. journal1, 2014, 29(3): 32-35. doi: 10.7668/hbnxb.2014.03.007.

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http://www.hbnxb.net/EN/Y2014/V29/I3/32

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