Quantitative Analysis of Five Selected Rumen Bacteria upon Incorporation Triticale Dried Distillers Grains with Solubles in Beef Cattle Diets

doi:10.7668/hbnxb.2014.02.019

Abstract

Abstract: The aim of this study was to explore the effects of triticale dried distillers grains with solubles (TD- DGS) incorporation in beef cattle diets on the relative abundance of five rumen bacteria viz., Fibrobacter succinogenes, Succinovibrio dextrinosolvens, Selenomonas ruminantium, Megasphaera elsdenii and Prevotella ruminicola. Real- time PCR was used to detect the gene expression of these five rumen bacteria. As compared with control group, the results showed that the relative abundance of F. succinogenes, S. ruminantium and M. elsdenii in 30% TDDGS group increased, and a 3-fold (P < 0. 05), a 10-fold (P < 0. 05) and a 2-fold (P < 0. 05) significant increase were ob- served respectively, however, the relative abundance of S. dextrinosolvens and P. ruminicola decreased in 30% TD- DGS group but no significant differences were found.

Key words: Cattle, Real-time PCR, Rumen microorganisms, Triticale, Dried distillers grains, Solubles

CLC Number:

S858.23

SU Rui, YANG Zhong, LI Chun, ZHANG Yan, ZHAO Peng-wei, WU Rui-bing, LI Jin-quan. Quantitative Analysis of Five Selected Rumen Bacteria upon Incorporation Triticale Dried Distillers Grains with Solubles in Beef Cattle Diets[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(2): 98-103. doi: 10.7668/hbnxb.2014.02.019.

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References

[1] Grubb J A, Dehority B A. Effects of an abrupt change inration from all roughage to high concentrate upon rumenmicrobial numbers in sheep[J]. Appl Microbiol,1975,30(3):404-412.
[2] Leedle J A Z, Bryant M P, Hespell R B. Diurnal variationsin bacterial numbers and fluid parameters in ruminal con-tents of animals fed lowor high-forage diets[J]. Appl En-viron Microbiol,1982,44(2):402-412.
[3] Dehority B A, Tirabasso P A, Grifo A P. Most-probable-number procedures for enumerating ruminal bacteria, in-cluding the simultaneous estimation of total and cellulo-lytic numbers in one medium[J]. Appl Environ Microbi-ol,1989,55(11):2789-2792.
[4] Wang S, Thomas K C, Sosulski K, et al. Grain pearlingand very high gravity (VHG) fermentation technologiesfor fuel alcohol production from rye and triticale [J].Process Biochem,1999,34(5):421-428.
[5] Linn J G, Chase L. Using distillers grains in dairy cattlerations[A]. Dubuque: In Proceeding of Professional DairyManagement Conference,1996: 1-11.
[6] Schingoethe D J, Brouk M J, Birkelo C P. Milk productionand composition from cows fed wet corn distillers grains[J]. J Dairy Sci,1999,82(3):574-580.
[7] Liu C, Schingoethe D J, Stegeman G A. Corn distillersgrains versus a blend of protein supplements with or with-out ruminally protected amino acids for lactating cows[J]. J Dairy Sci,2000,83(9):2075-2084.
[8] Anderson J L, Schingoethe D J, Kalscheur K F, et al. E-valuation of dried and wet distillers grains included at twoconcentrations in the diets of lactating dairy cows[J]. JDairy Sci,2006,89(8):3133-3142.
[9] Greter A M, Penner G B, Davis E C, et al. Effects of re-placing corn dry distillers grains with triticale dry distillers grains on lactation performance and plasma metabolites ofdairy cows[J]. Can J Anim Sci,2008,88(1):129-132.
[10] Fron M, Madeira H, Richards C, et al. The impact offeeding condensed distillers byproducts on rumen micro-biology and metabolism[J]. Anim Feed Sci Tech,1996,61(1):235-245.
[11] Mosoni P, Chaucheyras-Durand F, Bera-Maillet C, et al.Quantification by real time PCR of cellulolytic bacteriain the rumen of sheep after supplementation of a foragediet with readily fermentable carbohydrates: effect of ayeast additive [J]. J Appl Microbiol, 2007, 103 (6 ):2676-2685.
[12] Ohene-Adjei S, Chaves A V, McAllister T A, et al. Evi-dence of increased diversity of methanogenic archaeawith plant extract supplementation [J ]. Microb Ecol,2008,56(2):234-242.
[13] Tajima K, Aminov R I, Nagamine T, et al. Diet-depend-ent shifts in the bacterial population of the rumen re-vealed with Real-time PCR[J]. Appl Environ Microbiol,2001,67(6):2766-2774.
[14] Stevenson D M, Weimer P J. Dominance of Prevotellaand low abundance of classical ruminal bacterial speciesin the bovine rumen as revealed by relative quantifica-tion Real-time PCR [J]. Appl Microbiol Biotechnol,2007,75(1):165-174.
[15] Yu Y, Kim J, Hwang S. Use of Real-time PCR for group-specific quantification of aceticlastic methanogens in an-aerobic processes: population dynamics and communitystructures[J]. Biotechnol Bioeng, 2006, 93 (3): 424 -433.
[16] Weimer P J, Stevenson D M, Mertens D R, et al. Effect ofmonensin feeding and withdrawal on populations of indi-vidual bacterial species in the rumen of lactating dairycows fed high-starch rations[J]. Appl Microbiol Biotech-nol,2008,80(1):135-145.
[17] Stewart C S, Flint H J, Bryant M P. The Rumen Microbi-al Ecosystem[M]. London: Blackie Academic and Pro-fessional,1997:23-37.
[18] Russel J B, Rychlik J L. Factors that alter rumen micro-bial ecology [J]. Science, 2001, 292 (5519 ): 1119 -1122.
[19] Counotte G H M, Prins R A, Janssen R H A, et al. Roleof Megasphaera elsdenii in the fermentation of DL-[2-13C] lactate in the rumen of dairy cattle[J]. Appl Envi-ron Microbiol,1981,42(4):649-655.
[20] Mackie R I, Gilchrist F M C. Changes in lactate-produ-cing and lactate utilizing bacteria in relation to pH in therumen of sheep during stepwise adaptation to a high-con-centrate diet[J]. Appl Environ Microbiol,1979,38(3):422-430.
[21] Krause D O, Smith W J, Conlan L L, et al. Diet influ-ences the ecology of lactic acid bacteria and Escherichiacoli along the digestive tract of cattle: neural networksand 16S rDNA[J]. Microbiol,2003,149(Pt1):57-65.
[22] Klieve A V,Hennessy D,Ouwerkerk D,et al. Establishingpopulations of Megasphaera elsdenii YE 34 and Butyrivibrio fibrisolvens YE 44 in the rumen of cattle fedhigh grain diets [J]. J Appl Microbiol, 2003, 95 (3 ):621-630.

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