Identification of 5 PRatylenchus Species Utilizing DNA BaRcoding Method

doi:10.7668/hbnxb.2013.06.024

Abstract

Abstract: The root lesion nematodes,Pratylenchus Flipjev,1936,are among the widespread and seriously de-structive plant endoparasites around the world.Due to the instability of morphological characteristics, it was very dif-ficult to identify this kind of nematodes just by their morphology.In this study, 18 populations of the following 5 Pra-tylenchus species,i.e.Pratylenchus penetrans,P.coffeae,P.vulnus,P.scribneri and P.neglectus, were used as ex-periment materials, and their ribosomal ITS sequences were obtained to conduct blast alignment and phylogenetic a -nalysis with other ribosomal ITS sequences of Pratylenchus species submitted in GenBank in order to verify the fea-sibility of ITS regions as DNA barcode.The results showed that DNA barcoding approach using ribosomal ITS re-gions as target fragment could be readily used for identification of the above-mentioned 5 Pratylenchus species.

Key words: PRatylenchus, ITS, Phylogeny, DNA baRcoding

CLC Number:

S432.45

WEI Ya-dong, RONG Wan-tao, ZHAO Li-rong, WANG Jin-cheng, HUANG Guo-ming, GUO Jing-ze, SUN Jian-hua. Identification of 5 PRatylenchus Species Utilizing DNA BaRcoding Method[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(6): 136-139. doi: 10.7668/hbnxb.2013.06.024.

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