The Construction of Plant Overexpression Vector of Wheat TaNADP-ME2 Gene and Genetic Transformation of Rice

doi:10.3969/j.issn.1000-7091.2013.03.011

Abstract

Abstract: The wheat TaNADP-ME2 was not only responsive to light but also to drought， salt and low temperature．The recombinant plant overexpression vector was constructed， transformed into rice and acquired transgenic rice plant． The vector was constructed by recombinant technology． The agrobacterium was transformed by freeze-thaw method． The callus induced from the mature embryo of rice was transformed by agrobacterium-mediated method． The transgenic rice was identified by hygromycin and PCR method． The results revealed that recombinant plant overexpression vector pSUE2 has been constucted succeedingly and transformed into EHA105． The 16 transgenic rice positive plants were acquired by the PCR detection from HPT and TaNADP-ME2 gene． This research laid a foundation for identification the function of TaNADP-ME2 gene in enhancing water use efficiency in drought tolerance．

Key words: TaNADP-ME2, Agrobacterium, Gene transformation, Rice

CLC Number:

Fu Zhenyan, Gou Xiaoqing, Zhang Zhengbin, Xiao Xiangwen, Wang Xiaojun. The Construction of Plant Overexpression Vector of Wheat TaNADP-ME2 Gene and Genetic Transformation of Rice[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(3): 58-61. doi: 10.3969/j.issn.1000-7091.2013.03.011.

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http://www.hbnxb.net/EN/Y2013/V28/I3/58

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