Sequence and Expression Analysis of LEAP-2 Gene from Paralichthys olivaceus

doi:10.3969/j.issn.1000-7091.2012.z1.003

Abstract

Abstract: Antimicrobial peptides are found in several kinds of tissues of many vertebrates and invertebrates． These peptides are predicted to operate as a first-line host defense mechanism exerting broad-spectrum activity against pathogenic bacteria， fungi， parasites， and enveloped viruses． Followed basic local alignment search to the flounder EST library of GenBank database by using LEAP-2 from Oncorhynchus as probe sequence， an cDNA sequence owned a complete open reading frame of antimicrobial peptides was achieved． The sequence was verified byRT-PCR and named LEAP-2， accession number of EU586111 was acquired after sequence annotation and submittingto the GenBank database． Flounder LEAP-2 gene encode a peptide of 102 amino acids with a 28 amino acids signalpeptide and a mature peptide of 74 amino acids， with a predicted mulecular weight of 11 276． 9 u and pI at 9． 33 ．The amino acid and nucleotide sequences were conserved between flounder and other organisms and the homologyranged 38． 2% － 61 ． 7% ， the 57 － 92 amino acids are highly conserved region is to maintain the spatial structure，including four conserved cysteine， may be composed of two disulfide bonds． The flounder LEAP-2 gene is expressedin a wide range of tissues and predominantly expressed in the liver． Its expression is developmentally regulated withno detection in early stages of metamorphosis but highly in the mid-metamorphosis．

Key words: Paralichthys olivaceus, Liver-expressed antimicrobial peptide 2, Sequence analysis, Homology modelling

CLC Number:

S917.4

CHEN Xiao-wu, SHI Zhi-yi, LI Qian, JIANG Shan. Sequence and Expression Analysis of LEAP-2 Gene from Paralichthys olivaceus[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.3969/j.issn.1000-7091.2012.z1.003.

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