Isolation and Expression Analysis of a 14-3-3 Gene from Pyrus pyrifolia Nakai cv. Huobali

doi:10.3969/j.issn.1000-7091.2012.03.011

Abstract

Abstract: Based on the EST sequence encoded the 14-3-3，gene-specific primer was designed and used to obtain the full-length cDNA of a novel 14-3-3 gene from Pyrus pyrifolia Nakai cv. Huobali in Yunnan province with?the method of rapid amplification of cDNA ends (RACE). This novel gene was named as Pp14-3-3. Pp14-3-3 is?1 107 bp in length with an ORF of 786 bp，a 5'-untranslated region (UTR) of 84 bp，and a 3'-UTR of 237 bp，and?the ORF encodes a predicted polypeptide of 261 amino acids. The Pp14-3-3 shares higher homology with the known?14-3-3 proteins，and possesses the basic stucture of 14-3-3 proteins. A phylogenetic analysis of the relationship of?the newly identified Pp14-3-3 with some known 14-3-3s from other species grouped the Pp14-3-3 into the class of?non-ε 14-3-3s. Pp14-3-3 is abundantly expressed in pericarps of Huobali regardless received sunlight or not，and also expression in the young leaves. Isolation and expression analysis of Pp14-3-3 in this study laid the groundwork for?further studying on function of Pp14-3-3 .

Key words: Pyrus pyrifolia Nakai cv. Huobali, Rapid amplification of cDNA ends, RT-PCR, 14-3-3

CLC Number:

WANG Guang-yong, LIU Di-qiu, LI Min, RAO Jian, SUN Bing-zhao, DING Yuan-ming. Isolation and Expression Analysis of a 14-3-3 Gene from Pyrus pyrifolia Nakai cv. Huobali[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(3): 55-61. doi: 10.3969/j.issn.1000-7091.2012.03.011.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.3969/j.issn.1000-7091.2012.03.011

http://www.hbnxb.net/EN/Y2012/V27/I3/55

References

[1] Ferl R J，Manak M S，Reyes M F. The 14-3-3s[J]. Genome Biology，2002，3(7):3010. 1－3010. 7.
[2] Muslin A J，Tanner J W，Allen P M，et al. Interaction of?14-3-3 with signaling proteins is mediated by the recognition of phosphoserine[J]. Cell，1996，84(6):889－897.
[3] Yaffe M B，Rittinger K，Volinia S，et al. The structural basis for 14-3-3: phosphopeptide binding specificity[J].?Cell，1997，91(7):961－971.
[4] 曾?妍，郑志红，杨?磊. 14-3-3 蛋白功能研究进展
[J]. 现在肿瘤医学，2008，16 (7):1236－1238.
[5] Weiner H，Kaiser W M. 14-3-3 proteins control proteolysis?of nitrate reductase in spinach leaves[J]. Febs Lett，?1999，455:75－78.
[6] Toroser D，Athwal G S，Huber S C. Site-specific regulatory?interaction between spinach leaf sucrose-phosphate synthase and 14-3-3 proteins[J]. Febs Lett，1998，435(1):?110－114.
[7] Moorhead G，Douglas P，Cotelle V，et al. Phosphorylationdependent interactions between enzymes of plant metabolism and 14-3-3 proteins[J]. Plant J，1999，18(1):1－12.
[8] ?Olsson A，Svennelid F，Ek B，et al. A phosphothreonine?residue at the C-terminal end of the plasma membrane?H+-ATPase is protected by fusicoccin-induced 14-3-3?binding[J]. Plant Physiol，1998，118(2):551－555.
[9] ?Testerink C，van der Meulen R M，Oppedijk B J，et al.?Differences in spatial expression between 14-3-3 isoformsin germinating barley embryos[J]. Plant Physiol，1999，?121(1):81－87.
[10] de Vetten N C，Lu G，Feri R J. A maize protein associated with the G-box binding complex has homology to?brain regulatory proteins[J]. Plant Cell，1992，4(10):?1295－1307.
[11] Lu G，DeLisle A J，de Vetten N C，et al. Brain proteins?in plants: an Arabidopsis homolog to neurotransmitter?pathway activators is part of a DNA binding complex
[J]. Proc Natl Acad Sci U S A，1992，89(23):11490－11494.
[12] Schultz T F，Medina J，Hill A，et al. 14-3-3 proteins are?part of an abscisic acid-VIVIPAROUS1 (VP1) response?complex in the Em promoter and interact with VP1 and?EmBP1[J]. Plant Cell，1998，10(5):837－847.
[13] Chen Z，Fu H，Liu D，et al. A NaCl-regulated plant gene?encoding a brain protein homology that activates ADP ribosyltransferase and inhibits protein kinase C[J]. Plant?J，1994，6(5):729－740.
[14] Jarillo J A，Capel J，Leyva A，et al. Two related low-temperature-inducible genes of Arabidopsis encode proteins?showing high homology to 14-3-3 proteins，a family of?putative kinase regulators[J]. Plant Mol Biol，1994，25?(4):693－704.
[15] ?Camoni L，Harper J F，Palmgren M G. 14-3-3 proteins?activate a plant calcium-dependent protein kinase (CDPK)[J]. Febs Lett，1998，430(3):381－384.
[16] Yan J，He C，Wang J，et al. Overexpression of the Arabidopsis 14-3-3 protein GF14 lambda in cotton leads to a?"stay-green" phenotype and improves stress tolerance?under moderate drought conditions[J]. Plant Cell Physiol，2004，45(8):1007－1014.
[17] Liu D Q，Zhang X L，Tu L L，et al. Isolation by suppression subtractive hybridization of genes preferentially expressed during early and late fiber development stages in?cotton[J]. Mol Biol，2006，40:741－749.
[18] ?Aitken A. 14-3-3 proteins:A historical overview[J].?Seminars in Cancer Biology，2006，16(3):162－172.
[19] Wu K，Rooney M F，Ferl R J. The Arabidopsis 14-3-3?multigene family[J]. Plant Physiol，1997，114(4):1421－1431.
[20] 金谷雷，汪旭升，朱?军. 水稻 14-3-3 蛋白家族的生?物信息学分析[J]. 遗传学报，2005，32(7):726－732.
[21] Li X，Dhaubhadel S. Soybean 14-3-3 gene family:identification and molecular characterization [J]. Planta，?2011，233(3):569－582.
[22] Bihn E A，Paul A L，Wang S W，et al. Localization of 143-3 proteins in the nuclei of arabidopsis and maize[J].?Plant J，1997，12(6):1439－1445.
[23] Bunney T D，van Walraven H S，de Boer A H. 14-3-3?protein is a regulator of the mitochondrial and chloroplast?ATP synthase[J]. Proc Natl Acad Sci USA，2001，98?(7):4249－4254.
[24] ?Sehnke P C，Henry R，Cline K，et al. Interaction of a?plant 14-3-3 protein with the signal peptide of a thylakoid-targeted chloroplast precursor protein and the presence of 14-3-3 isoforms in the chloroplast stroma[J].?Plant Physiol，2000，122(1):235－242.
[25] Zhang Z T，Zhou Y，Li Y，et al. Interactome analysis of?the six cotton 14-3-3s that are preferentially expressed in?fibres and involved in cell elongation[J]. J Exp Bot，?2010，61(12):3331－3344.
[26] 黄仁华，陆云梅，夏仁学. 温州蜜柑果实发育与贮藏?过程中活性氧代谢研究[J]. 安徽农业科学，2009，37?(5):1914－1916.
[27] ?Zhang H，Wang J，Nickel U，et al. Cloning and expression of an Arabidopsis gene encoding a putative peroxisomal ascorbate peroxidase[J]. Plant Mol Biol，1997，34?(6):967－971.
[28] Zhang H，Wang J，Goodman H M. An Arabidopsis gene?encoding a putative 14-3-3-interacting protein，caffeic?acid/5-hydroxyferulic acid O-methyltransferase[J]. Biochim Biophys Acta，1997，1353(3):199－202.

Please choose a citation manager

Content to export