Cloning and Expression Pattern Analysis of GmHKT6 ;2 in Soybean

doi:10.3969/j.issn.1000-7091.2012.03.001

Abstract

Abstract: In order to investigate the salt tolerance mechanism of the HKT protein genes in soybean，a novel soybean gene GmHKT6;2 was identified in a salt tolerant soybean cultivar. GmHKT6;2 containing an open reading frame( ORF) of 1 644 nucleotides. The ORF encoded a protein of 547 amino acids. Sequence alignment and evolutionary tree clustering analysis showed that GmHKT6;2 is a new HKT protein gene in soybean. GmHKT6;2 gene were expressed in root，stem and leaves by using Real-Time PCR analysis in soybean. GmHKT6;2 was strongly induced and highly expressed in root，stem and leaves after treatment by 150 mmol/L NaCI. Domain analysis showed that:the soybean GmHKT6;2 gene with 10 putative transmembrane domain(TMD)and cation transport protein conserved domain，suggesting that GmHKT6;2 gene may be through control canon transporter to regulate the salt tolerance in soybean.

Key words: Soybean, Salt tolerance, Gene cloning, Expression analysis

CLC Number:

S565.1

CHEN Hua-tao, CHEN Xin, GU He-ping, CHEN Man-feng, ZHANG Hong-mei, YUAN Xing-xing, CUI Xiao-yan. Cloning and Expression Pattern Analysis of GmHKT6 ;2 in Soybean[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(3): 1-5. doi: 10.3969/j.issn.1000-7091.2012.03.001.

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