Cloning and Expression Analysis of GhGME Gene from Gossypium hirsuturm L.

doi:10.3969/j.issn.1000-7091.2012.01.003

Abstract

Abstract: On the basis of salt stress-related EST sequences of Gossypium hirsuturm L., a GDP-mannose-3', 5'-epimerase(GME) gene was isolated by the 3', 5'-RACE technology, named GhGME. The full-length cDNA of GhGME is 1467 bp, containing an 1131 bp ORF which encodes 376 amino acids. The relative molecular weight of GhGME protein is 42. 396 kDa, and its isoelectric point(pI) is 6. 291. Fluorescent quantitative real-time RT-PCR analysis of the expression of GhGME in roots, stems, leaves and three different treatments were employed. The results showed that the relative expression of GhGME was higher in roots and stems than that in leaves. And the expression of GhGME was up-regulated under either the 4℃ or 200 mmol/L NaCl treatment, but that was down-regulated under the 100 μmol/L GA₃ treatment. Differential expressions of GhGME under various treatments suggest that GhGME may play an important role in cotton acclimatization to environmental stresses. The response of GhGME to various stresses in this study indicates that GhGME should be further evaluated in fuction.

Key words: Gossypium hirsuturm L., Gene clone, GDP-mannose 3', 5'-epimerase, Expression analysis

CLC Number:

Q78
S562.03

WANG Jiang-ying, ZHANG Ning, SI Huai-jun, WU Jia-he. Cloning and Expression Analysis of GhGME Gene from Gossypium hirsuturm L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(1): 12-17. doi: 10.3969/j.issn.1000-7091.2012.01.003.

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References

[1] 程继东,安玉麟,孙瑞芬,等.抗旱、耐盐基因及其机理的研究进展[J] 华北农学报,2006,21(专辑):116-120.
[2] 刘金定,叶武威,樊宝相.我国棉花抗逆研究及其利用[J] 中国棉花,1998,25(3):5-6.
[3] Mittler R.Oxidative stress,antioxidants and stress tolerance[J] Trends Plant Sci,2002,7(9):405-410.
[4] Wheeler G L,Jones M A,Smirnoff N.The biosynthetic pathway of vitamin C in higher plants[J] Nature,1998,393(6683):365-369.
[5] Seki M,Carninci P,Nishiyama Y,et al.High-efficiency cloning of Arabidopsis full-length cDNA by biotinylated CAP trapper[J] The Plant Journal,1998,15(5):707-719.
[6] Ying D,Yuan Y,Jin-Yuan L.Molecular characterization of two rice cDNAs encoding GDP-mannose-3',5'-Epimerase and their expression patterns[J] Progress in Biochemistry and Biophysics,2005,33(4):368-376.
[7] 胡根海,喻树迅.利用改良的CTAB 法提取棉花叶片总RNA[J] 棉花学报,2007,19(1):19,69-70.
[8] 倪志勇,吕淑萍,李波,等.棉花GhCAD1 基因结构及表达分析[J] 华北农学报,2011,26(2):26-29.
[9] Li X B,Fan X P,Wang X L,et al.The cotton ACTIN1 gene is functionally expressed in fibers and participates in fiber elongation[J] The Plant Cell,2005,17(3):859-875.
[10] 秦斌,代其林,王劲.非生物胁迫下植物体内活性氧清除酶系统的研究进展[J] 绵阳师范学院学报,2009,28(11):50-53.
[11] 朱学艺,张承烈.植物响应水分胁迫的主要功能蛋白[J] 西北植物学报,2003,23(3):503-508.
[12] 邹礼平,陈锦华.植物抗坏血酸的合成和代谢以及相关酶基因的调控[J] 植物生理学通讯,2009,45(9):925-930.
[13] Wolucka B A,Persiau G,Van Doorsselaere J,et al.Partial purification and identification of GDP-mannose 3',5'-epimerase of Arabidopsis thaliana,a key enzyme of the plant vitamin C pathway[J] Proceedings of the National Academy of Sciences of the United States of America,2001,98(26):14843-14848.
[14] Wolucka B A,Van Montagu M.GDP-mannose 3',5'-epimerase forms GDP-L-gulose,a putative intermediate for the de novo biosynthesis of vitamin C in plants[J] The Journal of biological chemistry,2003,278(48):47483-47490.
[15] 邰付菊.棉花抗逆相关基因的克隆与功能研究及低温胁迫下棉花蛋白质差异表达分析[D] 武汉:华中师范大学,2007.
[16] 王美珍,王学敏,王赞,等.柠条GDP-甘露糖-3',5'-异构酶基因克隆、序列分析及其植物表达载体的构建[J] 中国草地学报,2009,31(5):75-83.

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